siRNA

MISSION® esiRNA Specifications

Quality Control

  • The amplified region of the cDNA with the highest possible number of highly effective siRNA is selected using DEQOR primer design program.
  • PCR product of transcriptome library tested via gel electrophoresis and identity confirmed by DNA sequencing.
  • Purified RNase III digested product (esiRNA) validated via gel electrophoresis.
  • esiRNA concentration determined by measuring OD260 or fluorescence intensity.

Purification

  • RNase III digested products are purified via column purification with Q-Sepharose, isopropanol precipitation and ethanol wash.

Format

  • All esiRNA are delivered in solution (resuspended in nuclease-free TE buffer) at the concentrations listed below, as measured by OD260 or fluorescence intensity.
  • esiRNA offering: 16,432 Human gene targets with 16,744 esiRNAs
    14,061 Mouse gene targets with 14,068 esiRNAs
  • Non-coding esiRNA libraries: 1,761 Human esiRNAs
                           643 Mouse esiRNAs

Available MISSION esiRNA Products

  Individual esiRNAs Plates MISSION esiRNAs esiRNA Genome Scale Libraries esiFLEX esiRNAs
Quantities Available 20 ug
50 ug
20 ug
50 ug
1 ug
2.5 ug
5.0 ug
1 ug
2.5 ug
5.0 ug
Gene target sources available Human
Mouse
Human
Mouse
Human
Mouse
Human
Mouse
Packaging 2 ml tubes 96-well plate (customizable) 96- or 384-well plate 96- or 384-well plate (customizable)
Concentration 200 ng/uL 200 ng/uL 50 ng/uL 50 ng/uL

 

  esiOPEN esiSEC Non-Coding esiRNA Libraries
Quantities available 20 ug
50 ug
20 ug
50 ug
1 ug
2.5 ug
5.0 ug
Gene target sources available All species Human
Mouse
Human
Mouse
Packaging 2 ml tubes 2 ml tubes 96-well plate
Concentration 200 ng/uL 200 ng/uL 50 ng/uL

Browse a complete list of esiRNA.

  • The standard plate format for MISSION esiRNA genome scale libraries are in 384-well plates, with all positions occupied.
  • The standard plate format for MISSION Non-Coding esiRNA libraries are in 96-well plates, with all positions occupied.

Shipping, Storage, and Stability

  • Shipped in solution on dry ice, in sealed plates.
  • esiRNA stored at –20 °C have demonstrated to be stable for 2 years. No decrease in stability after 12 freeze/thaw cycles.

Certificate of Analysis/Technical Data Sheet
esiRNA will be delivered with an esiRNA Technical Data Sheet, which includes:

  • Plate number and position
  • esiRNA ID number
  • Gene ID (Genbank) or Gene ID (Ensembl)
  • Gene name
  • Organism
  • OD (units)
  • Amount (μg)
  • Concentration (ng/μL)
  • Lot number
  • Target sequence
    • Additional data provided for purified RNase III digested product (esiRNA) upon request.
    • Custom, genome scale and non-coding esiRNA libraries will be delivered with a CD containing complete plating information.