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Cover image of LSI Issue 24
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(8 MB)
  Life Science Innovations - Issue 25

  • Targeted Genome Editing
  • Enhancing Lentiviral Transduction Efficiency 
  • siRNA Design, Quality and Delivery
  • TransPlex® RNA Amplification
  • Prestige Antibodies®

Life Science Innovations Issues

 

Issue 23

Life Science Quarterly and Origins Articles by Areas of Interest
Automated DNA Sequencing
Cell Biology
Cell Culture
Cell Signaling
Cloning and Expression
DNA and RNA Purification
Drug Discovery
Functional Genomics and RNAi
Gene Expression
Genomics
PCR
Protein Expression
Proteomics

Automated DNA Sequencing  
Title Article Type Issue
QuickComb™-96: A convenient Tool that Improves Sequence Data Application Note Volume 2, Issue 2,
April 2001

Cell Biology  
Title Article Type Issue
Prevalence of Scaffolds in Human Cytochrome P450 Inhibitors Identified Using the LOPAC1280 Library of Pharmacologically Active Compounds Application Note Volume 7, Issue 1,
Spring 2006
Leptomycin B: An Important Tool for the Study of Nuclear Export Application Note Volume 2, Issue 2,
April 2001
Leptomycin B: A Powerful Antibiotic Tool for Studying Nuclear Transport Application Note Volume 2, Issue 1,
January 2001
Isolation and Use of Mammalian Cell Nuclei Application Note Volume 1,
July 2000

Cell Culture Back to Top
Title Article Type Issue
Optimal Serum-Free Ex Vivo Expansion and Activation of T Lymphocytes Using Stemline™ T Cell Expansion Medium (176 K PDF) Application Note Volume 6, Issue 2,
Fall 2005
CHO CD-3 Chemically-Defined Medium Sustains Better Growth than Hydrolysate-Containing Media in Multiple CHO Recombinant Cell Lines Application Note Volume 6, Issue 1,
Spring 2005
Medium Optimization Kit for CHO Cells: Using Factorial Matrix Design of Experiment to Increase Cell Performance (199 K PDF) Feature Article Volume 5, Issue 3,
Fall 2004
Improved Ex Vivo Expansion of Functional CD34+ Cells Using Stemline™ II Hematopoietic Stem Cell Expansion Medium Feature Article Volume 5, Issue 2, Summer 2004
New Animal Component-Free, Protein-Free Medium for the Sf21 and Sf9 Insect Cell Lines Application Note Volume 5, Issue 2, Summer 2004
MegaCell™: The Next Generation of Classic Cell Culture Media Feature Article Volume 5, Issue 1, Winter 2004
TrypZean™: Recombinant Bovine Trypsin Expressed in Corn — A
Non-animal Alternative
Application Note Volume 5, Issue 1, Winter 2004
A Novel 2-Part System for the Culture of Bone Marrow Specimens for Cytogenetics Analysis Application Note Volume 4, Issue 2, Spring 2003
An Efficient Approach to Cell Culture Medium Optimization for
CHO Cells
Application Note Volume 4, Issue 2, Spring 2003
Serum-Free Expansion of CD34+ Umbilical Cord Blood Using Stemline™ Hematopoietic Stem Cell Expansion Medium Application Note Volume 4, Issue 1, Winter 2003
A New Medium for Adenovirus Production Application Note Volume 3, Issue 2, Summer 2002
Development and Customization of Protein-Free, Animal Component-Free Media for the Enhanced Expression of Recombinant Proteins in Chinese Hamster Ovary (CHO) Cells Application Note Volume 2, Issue 3,
July 2001
Performance-Optimized Hybridoma Medium: Replacing Serum and Other Animal-Derived Components Application Note Volume 2, Issue 2,
April 2001
Serum-Free Systems for MDBK and MDCK Epithelial Cells Application Note Volume 2, Issue 1,
January 2001
Growth Kinetics of Primary CEF Cells on Hillex Microcarriers in Sigma's TiterHigh™ CEF Basal Medium with 2% Bovine Calf Serum Application Note Volume 2, Issue 1,
January 2001

Cell Signaling Back to Top
Title Article Type Issue
Panorama™ Mouse/Rat Tissue Extract Protein Array Kit: A New Tool for Protein Expression Analysis Application Note Volume 6, Issue 1,
Spring 2005
Senescent Cells Staining Kit Application Note Volume 6, Issue 1,
Spring 2005
PKH Linker Kits for Fluorescent Cell Labeling Application Note Volume 5, Issue 3,
Fall 2004
Panorama™ Ab Microarray Cell Signaling Kit: A Unique Tool for Protein Expression Analysis Application Note Volume 5, Issue 2,
Summer 2004
The Effective Use of Protein Kinase Inhibitors Application Note Volume 5, Issue 1,
Winter 2004

Cloning and Expression Back to Top
Title Article Type Issue
Site-Specific Chromosomal Mutagenesis Using the TargeTron™ Gene Knockout System (179 K PDF) Application Note Volume 6, Issue 2,
Fall 2005
Superior Performance of HIS-Select™ HF Nickel Affinity Gel Application Note Volume 4, Issue 2,
Spring 2003
EZview™ Red Protein A and ANTI-FLAG® M2 Affinity Gels: Immunoprecipitation with Enhanced Visibility Affinity Beads Application Note Volume 2, Issue 3,
July 2001
S-Gal™: A Superior Dye to X-gal for Clonal Selection Feature Article Volume 2, Issue 2,
April 2001
Lysis of E. coli for the Purification of Soluble Recombinant Proteins using CelLytic-B™ and CelLytic-B™II Application Note Volume 2, Issue 2,
April 2001
3x FLAG: Ultra-Sensitive Detection of Recombinant Proteins Feature Article Volume 1,
July 2000

DNA and RNA Purification Back to Top
Title Article Type Issue
GenElute™ HP Endotoxin-Free Maxiprep Kit: High Quality Plasmid DNA for Transfecting Sensitive Eukaryotic Cell Lines Feature Article Volume 6, Issue 1,
Spring 2005
GenElute™ HP Endotoxin-Free Plasmid Maxiprep Kit Frequently Asked Questions (FAQs) Volume 6, Issue 1,
Spring 2005
GenElute™ Five-Minute Plasmid Miniprep Kit: The Fastest Method for Plasmid Preparation Application Note Volume 5, Issue 3,
Fall 2004
Extract-N-Amp™ Seed PCR Kit:Rapid Genomic DNA Extraction from Seeds Coupled with PCR Application Note Volume 5, Issue 1,
Winter 2004
Extract-N-Amp™ Tissue PCR Kit: Rapid Genomic DNA Extraction from Tissue Coupled with PCR Feature Article Volume 4, Issue 2,
Spring 2003
Extract-N-Amp™ Tissue PCR Kit Frequently Asked Questions (FAQs) Volume 4, Issue 2,
Spring 2003
GenElute™ High Performance (HP) Plasmid Kits: Plasmid DNA from a Midiprep or Maxiprep in 30 minutes Feature Article Volume 4, Issue 1,
Winter 2003
The Extract-N-Amp™ Blood Kit: Rapid DNA Extraction Coupled with PCR Feature Article Volume 3, Issue 2,
Summer 2002
One-Tube Preparation and PCR Amplification of DNA From Plant Leaf Tissue with Extract-N-Amp™ Application Note Volume 2, Issue 1,
January 2001
The GenElute™ Endotoxin-free DNA Purification Kit: Pure DNA for Endotoxin-Sensitive Applications Application Note Volume 1,
July 2000

Drug Discovery Back to Top
Title Article Type Issue
StarBright® Green Substrate: A Novel Fluorogenic Substrate for Alkaline Phosphatase Systems Application Note Volume 5, Issue 1, Winter 2004
Human Cytokine Gene Expression Analysis Utilizing Novel STATE® and HPSA™ Fluorescent Assay Technology Feature Article Volume 4, Issue 3,
Summer 2003
SigmaScreen™ or Cell-Based Assays Coated Multiwell Plates Application Note Volume 4, Issue 2,
Spring 2003

Functional Genomics and RNAi Back to Top
Title Article Type Issue
Examination of Efficacy, Specificity, and Efficiency of an shRNA Lentiviral Library
(299 K PDF)
Feature Article Volume 7, Issue 1,
Spring 2006
MISSION™ shRNA Library: Next Generation RNA Interference
(722 K PDF)
Feature Article Volume 6, Issue 2,
Fall 2005

Gene Expression Back to Top
Title Article Type Issue
Microarrays for Gene Discovery and Metabolic Pathway Analysis in Plants Feature Article Volume 2, Issue 1,
January 2001
Sigma's ArrayHyb™ and ArrayHyb™ LowTemp -Optimized Hybridization Buffers for Microarray Applications Application Note Volume 2, Issue 1,
January 2001

Genomics Back to Top
Title Article Type Issue
Balancing Histone/Protein Acetylation: Gene Regulation and Cancer Therapy (371 K PDF) Feature Article Volume 7, Issue 1,
Spring 2006
Amplification of Genome-Representative DNA from Limited Sources with GenomePlex® WGA Technology for Use in Genetic Alterations Studies (181 K PDF) Application Note Volume 7, Issue 1,
Spring 2006
Evaluation of DNA Extraction and Amplification Protocols to Investigate Bacterial Communities on Cultural Heritage (234 K PDF) Application Note Volume 7, Issue 1,
Spring 2006

PCR Back to Top
Title Article Type Issue
10x Bionic Buffer...Better, Faster, Stronger (167 K PDF) Application Note Volume 6, Issue 2,
Fall 2005
Real-time Quantitative Representation Measurement of Eighty Loci Using GenomePlex® Whole Genome Amplification (159 K PDF) Application Note Volume 6, Issue 2,
Fall 2005
Whole Gene Amplification from Archived Formalin-fixed, Paraffin-embedded Tissues Application Note Volume 6, Issue 1,
Spring 2005
Whole Genome Amplification Frequently Asked Questions (FAQs) Volume 5, Issue 3,
Fall 2004
Restorase™: A Novel DNA Polymerase Blend That Repairs Damaged DNA Application Note Volume 5, Issue 1,
Winter 2004
Quantitative PCR Using SYBR® Green JumpStart™ Taq ReadyMix™: Lights Your Way To Quantitative PCR and RT-PCR Application Note Volume 4, Issue 1,
Winter 2003
SYBR® Green JumpStart™ Taq ReadyMix™: Lights Your Way To Quantitative PCR Feature Article Volume 3, Issue 1,
Winter 2002
Improved Universal Vectorette System and Pre-Made Vectorette Genomic DNA Libraries Application Note Volume 3, Issue 1,
Winter 2002
Hot Start RT-PCR Results in Improved Performance of the Enhanced Avian RT-PCR System Feature Article Volume 2, Issue 3,
July 2001
JumpStart™ PCR -- Hot Start PCR Enzymes and Mixes Application Note Volume 2, Issue 1,
January 2001
Applications with REDTaq™ Application Note Volume 1,
July 2000
Sigma's New Enhanced Avian RT-PCR Kit Application Note Volume 1,
July 2000

Protein Expression Back to Top
Title Article Type Issue
HIS-Select™ iLAP™ Plate: A New Tool for the Rapid Screening of Histidine-tagged Proteins Directly from Bacterial Culture Application Note Volume 5, Issue 2, Summer 2004
RapidTransit™: A Convenient Method for Preparing Competent Cells Application Note Volume 5, Issue 2,
Summer 2004
FLAG® 96-well Immunoprecipitation System for High-Throughput Protein-Protein Interaction Studies Application Note Volume 4, Issue 3,
Summer 2003
Superior Performance of HIS-Select™ HF Nickel Affinity Gel Application Note Volume 4, Issue 2,
Spring 2003
HIS-Select™ Affinity Gels and Plates for Affinity Capture of Histidine-Containing Proteins Application Note Volume 3, Issue 2,
Summer 2002

Proteomics Back to Top
Title Article Type Issue
Novel ProteoPrep® 20 Immunoaffinity Depletion Resin for Human Plasma (394 K PDF) Technical Article Volume 7, Issue 1,
Spring 2006
Detergent Optimization for Total Protein Extraction from Chinese Hamster Ovary (CHO) Cells (208 K PDF) Application Note Volume 7, Issue 1,
Spring 2006
Cross-Clade CD8+ T-cell Responses with a Preference for the Predominant Circulating Clade (296 K PDF) Application Note Volume 7, Issue 1,
Spring 2006
Panorama™ Human p53 Functional Array (805 K PDF) Application Note Volume 6, Issue 2, Fall 2005
Superior Performance of a New Affinity Purified ANTI-FLAG M2 Antibody (173 K PDF) Application Note Volume 6, Issue 2, Fall 2005
Targeted Gene Knockdown Experiments: Combining 18O-Isotopic Labeling and Protein-AQUA™ (128 K PDF) Application Note Volume 6, Issue 2, Fall 2005
Atto Labels and Their Conjugates (165 K PDF) Application Note Volume 6, Issue 2, Fall 2005
18O Proteome Profiler Kit: A Useful Tool for Relative Quantitation of Global Differential Protein Expression Application Note Volume 6, Issue 1, Spring 2005
CelLytic™ Express: Whole Bacterial Culture Lysis for Affinity Purification Application Note Volume 5, Issue 3, Fall 2004
Development of a Novel Antibody-Based Resin for the Depletion of Human Albumin and IgG Application Note Volume 5, Issue 3, Fall 2004
C7BzO: A Powerful New Detergent for 2-D Gel Electrophoresis Sample Preparation Application Note Volume 5, Issue 2, Summer 2004
PNGase F Enzyme: Efficient N-Deglycosylation in Glycoprotein Analysis Application Note Volume 5, Issue 1, Winter 2004
Atto Dyes: Superior Fluors for Fluorescence Imaging and Conjugation Application Note Volume 5, Issue 1, Winter 2004
Superior Performance of the ProteoPrep™ Blue Albumin Depletion Kit for 2D Electrophoresis Application Note Volume 4, Issue 3, Summer 2003
Peptide Antigen Design for Antibody Production Application Note Volume 4, Issue 3, Summer 2003
Trypsin In-Gel Digest Kit: A Complete Kit for the Digestion of Proteins from SDS-PAGE Gels Application Note Volume 4, Issue 2,
Spring 2003
ProteoSilver™: High Sensitivity Silver Stain for SDS-PAGE Application Note Volume 4, Issue 1,
Winter 2003
Proteomic Applications of Specialty High Capacity Streptavidin-Coated Multiwell Plates Application Note Volume 4, Issue 1,
Winter 2003
Proteomics: New Products for Protein Analysis Application Note Volume 3, Issue 2,
Summer 2002
ProteoMass™ Peptide and Protein MALDI-MS Calibration Kit Application Note Volume 3, Issue 1,
Winter 2002

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