Biochemicals

Biofiles


Solutions for Detection
Volume 9

Abstract
Pooled shRNA screening using the highly validated MISSION® shRNA library provides a cost-effective method to investigate gene function and identify therapeutic targets. Here, we present data demonstrating the power and ease of use of the pooled shRNA approach to identify therapeutic targets.

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Recent Issues

Alternative Energy

BioFiles Volume 5 Number 5 - Enzymes and Reagents for Alternative Energy Cover

Enzymes and Reagents for Alternative Energy – Volume 5, Number 5

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  • Metabolic strategies in Alternative Energy
  • Enzyme-based Fuel Cells
  • Lignocellulosic Depolymerization
  • Enzymes for Biofuel Research
  • Reagents for Ethanol and BioDiesel Analysis

Antibodies

BioFiles Volume 3 Number 6 Antibodies Cover Image Antibodies (2.68 Mb PDF) – Volume 3, Number 6
  • Prestige Antibodies™
  • Antibodies to Actin and GAPDH
  • Antibodies to Fusion Tags
  • Arrays & Kits
  • Secondary Antibodies

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Antibiotics

Antibiotics for Research Applications Antibiotics for Research Applications (5.6 Mb PDF) – Volume 4, Number 3
  • Antimicrobial Peptides
  • Antifungals
  • New Antibiotics
  • Ready Made Solutions
  • Antibiotic Selector

 

BioFiles Volume 1 Number 4 Antibiotics for Research Applications Cover Image Antibiotics for Research Applications (2.25 Mb PDF) – Volume 1, Number 4
  • Ready Made Antibiotic Solutions
  • Antibiotics For Plant Tissue Culture
  • Vetranal Analytical Standards

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Cancer

Cancer Cancer Metabolism – Volume 7, Number 4

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  • Oncogenes and Tumor Suppressors Reprogram Metabolism
  • Aerobic Glycolysis and the Warburg Effect
  • Glutamine Metabolism
  • Fatty Acid Synthesis and Metabolism
  • Nucleotide Synthesis in Cancer Cells

 

BioFiles Volume 5 Number 6 - Cancer Cancer – Volume 5, Number 6

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  • New Cancer Biomarker Discovered
  • Tp53 Knockout Rat
  • Cancer Research: Epigenetic Mechanisms
  • Folic Acid Metabolism: A Role in Cancer's Cause and Cure
  • Cell Lines for Cancer Research

 

BioFiles Volume 4 Number 7 Cancer and Angiogenesis Cover Image Cancer and Angiogenesis – Volume 4, Number 7

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  • VEGF, VEGFR, PDGFR and Non-Receptor Tyrosine Kinase Inhibitors
  • Antibodies
  • Antioxidants and Cytoprotectants
  • Microtubule Inhibitors
  • DNA and RNA Transcription Regulation
  • DNMT and HDAC Inhibitors
  • Gene Expression by qRT-PCR

 

BioFiles Volume 3 Number 5 Cancer Origins and Carcinogenesis Cover Image Cancer Origins and Carcinogenesis (3.06 Mb PDF) –Volume 3, Number 5
  • The Cancer Stem Cell Hypothesis
  • Carcinogenesis and Epigenetics
  • Imprint™ Chromatin Immunoprecipitation Kit
  • Imprint DNA Modification Kit
  • MISSION® siRNA Druggable Genome Libraries
  • Carcinogen Selector

 

BioFiles Volume 2 Number 4 Cellular Mechanisms and Cancer Cover Image Cellular Mechanisms and Cancer (1.01 Mb PDF) – Volume 2, Number 4
  • Innovation @ Work
  • ABC Transporters and Cancer Drug Resistance
  • NF-kB and Innflammation
  • DNA Damage and Repair
  • New Products

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Carbohydrate Analysis

BioFiles Volume 2 Number 3 Complex Carbohydrate Analysis: Enzymes, Kits, and Reagents Cover Image Complex Carbohydrate Analysis: Enzymes, Kits, and Reagents (1.06 Mb PDF) – Volume 2, Number 3
  • Agarose
  • Alginic Acid
  • Cellulose Lichenin and Glucans
  • Hemicellulose and Xylan
  • Chitin and Chitosan
  • Chondroitins
  • Dextran
  • Heprarans
  • Hyaluronic Acid
  • Inulin
  • Peptidoglycan
  • Pectin
  • Pullulan
  • Starch and Glycogen

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Cell Culture

Cell Culture Contamination Cell Culture Contamination – Volume 7, Number 1

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  • Antibiotics
  • Mycoplasma Detection/Elimination
  • Endotoxin Testing
  • Cell Culture Equipment

 

BioFiles Volume 5 Number 8 - Cell-Based Assays Cover Cell-Based Assays – Volume 5, Number 8

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  • Assays and Reagents for Measuring Cytotoxicity, Proliferation, and Viability
  • Apoptosis
  • Autophagy
  • Reactive Oxygen Species
  • Lopac Library
  • Cell and Organelle Labeling
  • Cell Culture – Cell Lines
  • Cell Culture Labware

 

BioFiles Volume 5 Number 4 Cryopreservation Cover Image Cryopreservation – Volume 5, Number 4

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  • New Products
  • Cell Freezing Media and Reagents
  • ECACC Cell Lines
  • Protocol for the Cryopreservation of Cell Lines
  • Cryoware

 

BioFiles Volume 5 Number 3 Vaccine Adjuvants Cover Image Vaccine Adjuvants – Volume 5, Number 3

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  • Adjuvant Research
  • New Approaches to VaccineAdjuvants
  • Vaccine Media
  • Life Science Labware

 

BioFiles Volume 4 Number 5 Growth Factors and Cytokines Cover Image Growth Factors and Cytokines – Volume 4, Number 5

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  • Growth Factors
  • Cytokines
  • Fetal Bovine Serum

 

BioFiles Volume 3 Number 8 Attachment and Matrix Factors Cover Image Attachment and Matrix Factors (3.35 Mb PDF) –Volume 3, Number 8
• Attachment and Matrix Factors
• Cell Attachment
• Troubleshooting Guide
• 3-D Cell Culture Technology
• Corning® Plasticware

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Cell Dissociation and Lysis

 

BioFiles Volume 1 Number 2 Enzymes for Cell Dissociation and Lysis Cover Image Enzymes for Cell Dissociation and Lysis (4.49 Mb PDF) – Volume 1, Number 2
  • Detachment of Cultured cells
  • Lysis and Protoplast preparation of yeast, bacteria and plant cells
  • Permeabiliaztion of Mammalian Cells
  • Mitochondria Isolation

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Detection

 

Solutions for Detection – Volume 9

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  • Duolink® Using PLA® Technology
  • Prestige Antibodies®
  • Sunstone® Luminescent UCP Nanocrystals
  • CF™ Dyes
  • Mix-n-Stain™ Antibody Labeling Kit
  • Fluorescent Dye Comparison
  • Atto™ Dyes
  • Abberior® Dyes
  • Spectral Fluorescence Standard Kit

 

BioFiles Volume 6 Number 3 - Superior Fluorescent Products Superior Fluorescent Products – Volume 6, Number 3

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  • Atto Dyes for Superior Fluorescent Imaging
  • Analyzing Properties for Superior Fluorescent Dyes
  • Atto Dyes and Tracy Dyes for Fluorescent Protein Labeling
  • Fluorescent Multiplex Detection Using Antibody Atto Dye Conjugates
  • Sensitive Detection of poly(his)- tagged Proteins with Ni-NTA Atto Complex
  • Atto Dye Lectin Conjugates for Fluorescent Carbohydrate Labeleing
  • Sensor Dyes
  • New Products for Detection Applications

 

BioFiles Volume 5 Number 2 Protein Characterization and Detection Cover Image Protein Characterization & Detection – Volume 5, Number 2

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  • Protein Labeling
  • Crystallization
  • MALDI Mass Spectrometry
  • Isoelectric Focusing

 

BioFiles Volume 4 Number 1 Innovative Solutions for Fluorescent and Chemiluminescent Application Cover Image Innovative Solutions for Fluorescent and Chemiluminescent Application
– Volume 4, Number 1
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  • DNA Detection
  • NIR Fluorescence Dyes
  • Carbohydrate Detection
  • Protein Detection
  • Isoelectric Focusing
  • Detection Kits
  • Sensor Dyes

 

BioFiles Volume 3 Number 4 Substrate Systems of Enzyme Detection Cover Image Substrate Systems of Enzyme Detection (5.24 Mb PDF) – Volume 3, Number 4
  • Colorimetric Alkaline
  • Phosphatase and Peroxidase Substrate
  • Detection Systems
  • Luminescent Alkaline

 

BioFiles Volume 2 Number 5 Fluorescent and Chemiluminescent Techniques for Life Science Applications Cover Image Fluorescent and Chemiluminescent Techniques for Life Science Applications (2.81 Mb PDF) – Volume 2, Number 5
  • DNA and RNA Detection Techniques
  • Fluorescent Microarray Experiments
  • Fluorescence Multiplexing
  • Ni-NTA-Atto™ Conjugates
  • Ampliflu™ Red Peroxidase Substrate

 

BioFiles Volume 1 Number 3 Application Series: Fluorescent Detection Cover Image Application Series: Fluorescent Detection (829 Kb PDF) – Volume 1, Number 3
  • New Fluorescent Dyes for Electrophoresis
  • Labels and Conjugates
  • Single Molecule Detection with ATTO 647N NTA

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Detergents

 

BioFiles Volume 3 Number 3 Detergents and Solubilization Reagents Cover Image Detergents and Solubilization Reagents (3.54 Mb PDF) – Volume 3, Number 3
  • Detergent Properties and Applications
  • BioUltra Detergents
  • Cyclodextrins
  • Antifoams

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Epigenetics

 

Epigenetics Epigenetics – Volume 7, Number 3

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  • Introduction to DNA Methylation
  • DNA Methylation and Bisulfite Conversion
  • Global DNA Methylation Quantification
  • Modulators and Proteins for Methylation
  • Histone Modification and Chromatin Remodeling
  • Antibodies for Epigenetics Research
  • RNAi Tools for Epigenetics Research
  • CompoZr® Zinc Finger Nucleases

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Genomics

 

BioFiles Volume 3 number 1 Genomics Cover Image Genomics (2.96 Mb PDF) – Volume 3, Number 1
  • Genomics Workflow
  • RNA Purification
  • Plasmid DNA Purification
  • Genomic DNA Purification
  • Intergrated Extraction and Amplification
  • Whole Transcriptome Amplification
  • Whole Genome Amplification
  • Quantitative PCR

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Glycobiology

 

BioFiles Volume 5 Number 1 Glycobiology Cover Image Glycobiology – Volume 5, Number 1

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  • Sialic Acid Synthesis and Signaling
  • Mass Spectrometry of Glycans
  • Histological Detection of Pathogenic Fungi in Human Tissue
  • Select-HA™
  • New Products for Glycobiology and Glycochemistry

 

BioFiles Volume 3 Number 10 Glycobiology-Glycosaminoglycans and Polysaccharides Cover Image Glycobiology-Glycosaminoglycans and Polysaccharides (2.96 Mb PDF) – Volume 3, Number 10
  • Glycosaminoglycan Sulfation and Sign
  • Dextrans and Related Polysaccharides
  • GlycoProfile β-Elimination Kit

 

BioFiles Volume 2 Number 1 Glycobiology Cover Image Glycobiology (5.46 Mb PDF) – Volume 2, Number 1
  • Glycosyltransferase
  • Glycoprotein
  • Deglycosylation
  • Enzymes for Glycobiology
  • Glycoprofile™ Labeling Kits
  • Glycoprofile™ Azido Sugars
  • Glycan Standards
  • Galectins And Lectins

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Mass Spectrometry

 

Mass Spectrometry (PDF) – Volume 7, Number 7
  • Use of Proteomic Standards to Optimize Conditions for an
    MRM LC-MS Platform
  • Metabolomics Analysis and the METLIN Metabolite Database

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Metabolomics

 

Metabolic Syndrome and Insulin Resistance Metabolic Syndrome and Insulin Resistance – Volume 6, Number 4

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  • Insulin Signaling and Energy Homeostasis
  • Lipid Induced Insulin Resistance
  • Mitochondrial Dysfunction and Metabolic Defects
  • Mitochondrial Stress and Reactive Oxygen Species
  • Sigma-Aldrich Microarray Tools

 

BioFiles Volume 6 Number 1 - Drug Metabolism Drug Metabolism – Volume 6, Number 1

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  • Drug Transport
  • Phase 1 Drug Metabolism
  • Drug Conjugate Analysis

 

BioFiles Volume 5 Number 7 - Lipid Metabolism Lipid Metabolism – Volume 5, Number 7

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  • Natural Abundance Lipid Metabolites
  • Isotopically Labeled Metabolites
  • Characteristic Metabolites for Inborn Errors of Lipid Metabolism
  • Lipid Metabolites as Biomarkers
  • Altered Lipid Metabolites in Aging
  • Lipid Metabolites in Natural Product Biosynthesis

 

BioFiles Volume 4 Number 8 Metabolomics Cover Image Metabolomics – Volume 4, Number 8

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  • Natural Product Biosynthesis
  • Disease State Biomarkers
  • Inborn Error Metabolism
  • Staple Isotopes-Labeled Metabolic Studies

 

BioFiles Volume 3 Number 7 Stem Cell models for Drug Discovery and Metabolomics Research Cover Image Stem Cell models for Drug Discovery & Metabolomics Research (3.0 Mb PDF) – Volume 3, Number 7
  • Stemline® Stem Cell Media
  • Stem Cell Reagents and Antibodies
  • Drug Discovery
  • Metabolite Libraries
  • Stable Isotope Labeled Metabolites
  • Metabolite Chromatographic Analysis

 

BioFiles Volume 2 Number 7 Cholesterol Homeostasis Cover Image Cholesterol Homeostasis (2.8 Mb PDF) – Volume 2, Number 7
  • HMG-CoA Reductase (HMGR) Assay Kit
  • Cholesterol Biosynthesis
  • Blocking Absorption of Dietary Cholesterol
  • Cholesterol Transport
  • Bile Acids

 

BioFiles Volume 2 Number 6 Carbohydrate Metabolism and PPAR Signaling Cover Image Carbohydrate Metabolism and PPAR Signaling (2.69 Mb PDF) – Volume 2, Number 6
  • Glycolysis
  • Pyruvate Transformations
  • Citric Acid Cycle
  • Monosaccharide Biosynthesis
  • Pentose Phosphate Pathway
  • Calvin Cycle
  • PPAR Signaling

 

BioFiles Volume 1 Number 1 Kits and Reagents for Metabolomic and Dietary Research Cover Image Kits and Reagents for Metabolomic and Dietary Research (1.03 Mb PDF) – Volume 1, Number 1
  • Kits for Quantitation
  • New Products
  • Metabolite Libraries
  • Stable Isotope Metabolites

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Neuroscience

 

Neurodegenerative Diseases Neurodegenerative Diseases – Volume 7, Number 2

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  • Alzheimer's Disease: Antibodies, Proteins, Peptides and Assays
  • Huntington's Disease: Antibodies, Proteins, and Peptides
  • Parkinson's Disease: Antibodies, Proteins, and Peptides
  • Differential protein expression in rat models using the Panorama Neurobiology Array

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Nutrition Research

 

Dietary Bioactives Dietary Bioactives – Volume 7, Number 6

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  • Phytochemicals
  • Polyphenols
  • Phenols
  • Terpenes and Terpenoids
  • Glycosides
  • Alkaloids
  • Oils and Resin-derived
  • Compounds
  • Analytical Standards
  • Functional Foods
  • Fiber and Prebiotics
  • Omega-3 Fatty Acids
  • Vitamins
  • Stable Isotopes

 

BioFiles Volume 6 Number 2 - Nutrition Research Nutrition Research – Volume 6, Number 2

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  • Probiotics and Human Health
  • Metagenomics and Quantitative PCR
  • Prebiotics
  • Vitamins for Nutrition Research

 

BioFiles Volume 4 Number 6 Nutrition Research Cover Image Nutrition Research –Volume 4, Number 6

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  • Vitamins
  • Plant Bioactive Extraction and Isolation
  • Core Bioreagents

 

BioFiles Volume 3 Number 9 Nutrition Research Cover Image Nutrition Research (4.94 Mb PDF) – Volume 3, Number 9
  • Dietary Terpenes
  • Sweet Taste Perception
  • Bioactive Nutrient Explorer
  • Retinoic Acid and Gene Expression
  • Metabolite Libraries

 

BioFiles Volume 2 Number 2 Nutrition Research Cover Image Nutrition Research (841 Kb PDF) – Volume 2, Number 2
  • Dietary Antioxidants
  • Omega-3 Fatty Acids and Heart Disease
  • The Bioactive Nutrient Explorer
  • Metabolite Libraries

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Plasma and Blood

 

BioFiles Volume 1 Number 5 Plasma and Blood Related Proteins and Enzymes Cover Image Plasma and Blood Related Proteins and Enzymes – Volume 1, Number 5
  • Immunodepletion Kits for Plasma Protein Analysis
  • Plasma Derived Proteins and Enzymes
  • Cellular Derived Blood Proteins and Enzymes
  • Whole Sera and Plasma

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Protease

 

BioFiles Volume 4 Number 2 Protease Inhibition and Detection Cover Image Protease Inhibition and Detection (3.79 Mb PDF) – Volume 4, Number 2
  • Broad Spectrum Protease Inhibitors
  • Inhibitor Cocktails and Tablets
  • Inhibitor Specificity Index
  • Protease Detection

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Proteomics

 

BioFiles Volume 4 Number 4 Structural Proteins Cover Image Structural Proteins (13.9 Mb PDF) – Volume 4, Number 4
  • Cytoskeletal Proteins
  • Extracellular Matrix Proteins
  • Adhesion Proteins

 

BioFiles Volume 3 Number 2 Ultra-pure Protein Tools Cover Image Ultra-pure Protein Tools (3.13 Mb PDF) – Volume 3, Number 2
  • BioUltra Proteins, Enzymes and Peptides
  • Proteomics Grade Proteases
  • Universal Proteomics Standards
  • BioUltra Reagents

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Stem Cell Biology

 

BioFiles Volume 3 Number 11 Innovative Solutions for Stem Cell Biology Cover Image Innovative Solutions for Stem Cell Biology (2.7 Mb PDF) – Volume 3, Number 11
  • Stemline Media
  • 3D Cell Culture Matrices
  • Small Molecules
  • Antibodies
  • MISSION® Lentiviral Libraries
  • CompoZr ZFN Technology

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