Attention:

Certain features of Sigma-Aldrich.com will be down for maintenance the evening of Friday August 18th starting at 8:00 pm CDT until Saturday August 19th at 12:01 pm CDT.   Please note that you still have telephone and email access to our local offices. We apologize for any inconvenience.

Customer Education

Quantitative Image Analysis and Emerging Applications on the ImageStream Imaging Flow Cytometer Webinar

Webinar Title: Quantitative Image Analysis and Emerging Applications on the ImageStream Imaging Flow Cytometer


 

 What Does it Cover?

The ImageStream® and FlowSight® are multispectral imaging flow cytometers that generates high resolution images of cells at a rate of 1000’s of cells per second. This allows for the rapid acquisition of tens of thousands of images per sample. Using the IDEAS® image analysis software, the system calculates features based not only on fluorescence intensity but the morphology of that fluorescence as well. This novel approach is able to seamlessly combine the quantitative power of flow cytometry with the high content information associated with microscopy. The system can collect data on a wide range of applications including nuclear localization during a signal transduction cascade, measuring colocalization of two probes, or quantify features on the phagocytosed particles in macrophages.
 

 What Will You Learn?

  • Technology description
  • Why quantitative image analysis is important
  • Feature descriptions for critical morphologies
  • Emerging applications
  • IDEAS masking enhancements

 Who Should Attend?

ImageStream and FlowSight users, as well those interested in how Image Cytometry can advance their science.

Speaker Bio
Brian Hall
Senior Scientist at MilliporeSigma 
Brian Hall is a senior scientist at Amnis Corporation now MilliporeSigma and has been working on the ImageStream technology for the past 15 years. As one of the original Amnis team members, he’s gained the experience of developing the hardware systems from a prototype, helped write the technical manuals, designed the training courses and conduced seminars and poster presentations the world over. He has 6 patents and over 20 publications using quantitative image Cytometry and continues to collaborate with researchers developing novel applications for the ImageStream. He is a graduate of Boston University, and spent the early part of his career doing cancer research at both the Fox Chase Cancer Research Center in Philadelphia and the Fred Hutchinson Cancer Center in Seattle. After specializing in Flow Cytometry and confocal microscopy he moved to Amnis and continues to develop and instruct on the strengths of Imaging Flow Cytometry.