At MilliporeSigma we are problem solvers, who collaborate with the world’s leading scientists to solve the toughest challenges in Life Science.

Join us to learn about recent innovations and discuss how we can work together to solve the impossible.

Date: Tuesday, September 12, 2017

Time: 9:00 AM - 12:00 PM including Q&A

Registration and Check-in: 8:45 AM

Location: University of California San Francisco
Genentech Hall Auditorium, Mission Bay Campus



 Lecture 1: Transforming science through technology: how the coming disruption will speed
 everything up (9:00 AM – 10:00 AM)

It’s never been a more exciting time to be at the convergence of science and technology, where a radical transformation is occurring. In our personal lives, we’re getting used to a more frictionless world, yet there remains a gap between the digital experiences we enjoy at home and the ones we often have in the lab. And it’s about time the lab catch up.

MilliporeSigma has always been about helping scientists solve the toughest problems and we believe when labs are fully connected and data enabled, the focus can be placed on science and progress and less on the process.

This presentation will highlight many of the emerging technology trends that will impact how science is done, including: smarter, connected devices and consumables, wearables, and real-time data analysis. It will end with a brief overview of the digital capabilities that MilliporeSigma is building to bring about greater connectivity & smarter data delivery to the modern lab environment.
 

Speaker Bio
Michael Vande Vrede
Head of M Connected Labs Promise Venture
MilliporeSigma
Michael Vande Vrede, has a background in biology and international business. He has spent the past 10 years working on digital transformation projects in the Life Science space. He's passionate about modernizing the lab and solving the manual pain points that occur in labs today. Currently he is leading a new digital initiative for MilliporeSigma called Connected Lab.

 Lecture 2: Advanced CRISPR/Cas9 Genome Editing Technology in Mammalian Systems
 (10:00 AM – 11:00 AM)

CRISPR/Cas9 is a genomic editing system that has revolutionized biomedical research with its elegant design and efficient method of RNA-guided gene targeting. This seminar will examine the CRISPR/Cas9 system and some of its practical applications for genome engineering. It will include an overview of CRISPR technology, with an emphasis on design, efficiency, specificity and use in gene knockout and targeted integration. The benefits of using CRISPR technology to generate knockout, conditional knockout and knock-in transgenic animal models will be discussed, along with various methods to optimize this process. New CRISPR formats, including epigenetic activators, synthetic guide RNA, enhanced-specificity Cas9 protein, and pooled or arrayed lentiviral screening libraries also will be covered.

Seminar Outline:

CRISPR/Cas9 System

  • Background & mechanism
  • Off-target reduction

Applications

  • Gene knockout & insertion
  • Transgenic animal models

New CRISPR Formats

  • Epigenetic activators
  • Synthetic gRNA & eSpCas9
  • Pooled & arrayed LentiCRISPR libraries
Speaker Bio
Antonia Livolsi
Research Application Scientist
MilliporeSigma
Antonia received her Ph.D in Life Sciences from the University of Nice-Sophia Antipolis, France, focusing on T cell signaling and cancer biology. She pursued post-doctoral research in Gene Expression at the Salk Institute and at UCSD in CA before joining Millipore Sigma in 2007. In her current Research Technology Specialist role, she is acting as a strategic scientific partner to customers in the West Coast region and supporting research with MilliporeSigma's innovative life science technology portfolio, including Functional Genomics, Stem Cells and Epigenetics-related workflows.

 Lecture 3: The Human Protein Atlas (11:00 AM – 12:00 PM)

Spatial partitioning of biological functions is a phenomenon fundamental to life. The Human Protein Atlas utilizes an antibody-based approach to explore the hierarchy of specialized systems ranging across all scales, from organs to specialized cells, from cells to organelles. Antibodies are used for immunofluorescence staining on cell lines for determination of spatial distribution at a subcellular level, as well as for immunohistochemistry on tissue microarrays for distribution of the protein expression in normal and cancer tissues. The open access database contains millions of high-resolution images, released together with application-specific validation performed for each antibody.  In this presentation we will focus on the recently released Cell Atlas where 10,000 Prestige Antibodies® have been used to map the distribution of corresponding proteins.
 

Speaker Bio
Charlotta Agaton
Product Manager
Atlas Antibodies
Charlotta is one of the pioneers within the Human Protein Atlas project that started in 2003. Her thesis was the pilot of the project. Charlotta holds a PhD in Biotechnology from the Royal Institute of Technology, Stockholm, Sweden and has been working within and in close collaboration with the Human Protein Atlas project since it started. She joined Atlas Antibodies in 2007 and is the Product Manager of the Human Protein Atlas antibodies.

 

Speaker Bio
Mikael Malmqvist
Senior Research Scientist
Atlas Antibodies
Mikael has a long background within molecular and cell biology research and drug discovery. He was awarded a PhD studentship at King’s College London investigating the molecular causes of abnormal protein aggregation in Alzheimer’s disease and continued as a postdoctoral researcher at the Neuroscience Drug Discovery Unit at the Wolfson CARD King's College London testing a library of novel compounds in experimental models of Alzheimer’s disease. Before joining Atlas Antibodies to manage the immunofluorescent platform for quality assurance of antibodies, Mikael performed further postdoctoral research studies at Uppsala University related to HPV-induced cervical cancer.