| Biology Webinar Guide |
| Title |
Topics Covered |
Length |
| Proteomics |
| Winning Westerns |
western blot, protein, troubleshooting, immunoblotting, electrophoresis |
60 minutes |
| Human Protein Atlas Webinar |
proteome, antibody, biomarker |
36 minutes |
| qPCR |
Primer and Probe Design
|
primer, probe, assay design
|
7 minutes
|
| An Introduction to qPCR Concepts |
gene expression, mRNA, assay design |
10 minutes |
| Selecting a qPCR Basic Detection Chemistry |
assay design, molecular beacons, scorpion probes, locked nucleic acid probes |
13 minutes |
| Choosing a Fluorophore/Quencher Combination |
assay design, probe design |
12 minutes |
| Chemistries for More Challenging qPCR Assays |
assay design, optimization, probe design |
15 minutes |
| MIQE Concepts |
assay standardization, assay reproducibility |
3 minutes |
| Reference Gene Validation |
normalization, reference gene, NormFinder, GeNorm, GenEX |
13 minutes |
| Data Analysis Guidelines |
absolute quantification, relative quantification, statistical analysis |
11 minutes |
| MIQE: Assay Design Considerations |
MIQE, assay design, sample quality, optimization |
18 minutes |
| MIQE: Sample Derived Inhibitors |
sample quality, inhibitors, assay design, SPUD assay |
13 minutes |
| MIQE: RNA Quality Considerations |
RNA quality, assay design, integrity assay |
16 minutes |
| MIQE: RNA Quantity and RT Considerations |
RNA quantification, reverse transcription |
17 minutes |
| PCR Webinar Series: Basic Principles of PCR, qPCR and ddPCR |
PCR, qPCR, ddPCR, choice of detection method |
53 minutes |
| PCR Webinar Series: qPCR Assay Design |
evaluation of pre-designed assays, assay design, amplicon and transcript selection, primers, probe design, reverse transcription protocols |
56 minutes |
| PCR Webinar Series: Importance of Sample Quality in qPCR Analysis |
sample quality |
51 minutes |
| PCR Webinar Series: Improving Assay Quality by Optimization and Validation |
assay optimization, assay validation |
50 minutes |
| PCR Webinar Series: Data Analysis |
qPCR data analysis, absolute quantification, relative quantification, expression profiling |
61 minutes |
| PCR Webinar Series: Troubleshooting |
assay troubleshooting |
61 minutes |
| CRISPR |
|
|
| Methods for Validating a Gene Editing Approach to Treating Brain Disease with Zinc Fingers, TALEs, and CRISPR/Cas9 |
CRISPR, Cas9, TALEs, ZFN, Genome Editing, Neuroscience, quantitative Western blot |
60 minutes *broadcast
November 9, 2016
– Register now* |
| Designing the Next Phase in Genome Editing: Advanced CRISPR Applications |
CRISPR, Cas9, gRNA, Genome Editing |
43 minutes
|
| Sigma Pooled CRISPR Screening for Functional Genomics |
CRISPR, pooled screening, cell culture, FACS, genome editing |
50 minutes
|
| Sigma CRISPR Paired Nickases for Genome Editing |
CRISPR/Cas9, Specificity, Paired Nickase |
29 minutes |
| Practical Utilization of CRISPR in the Laboratory Workflow |
CRISPR, Cas9, Genome Editing, Cell Engineering |
33 minutes |
| Sigma Pooled CRISPR Screening for Functional Genomics |
CRISPR, pooled screening, cell culture, FACS, genome editing |
49 minutes
|
| Genome Editing with CRISPR Systems |
CRISPR/cas9, genome editing |
61 minutes |
| SygRNA™-Synthetic Two Part CRISPR RNA System |
Cas9, RNA, Genome Editing, sgRNA sequencing |
58 minutes |
| Sanger Arrayed CRISPR Screening in Partnership with Evotec |
Evotec Partnership, Lentiviral CRISPR KO libraries, CRISPR pooled |
50 minutes |
| Cell Culture |
|
|
Advanced Cell Culture Technology for Generation of In Vivo-like Tissue Models
|
2D vs 3D cell culture debate, Review of alternative approaches and the development of new technologies, Challenges facing 3D culture methods, in terms of technologies available and methods used, Showcase applications where 3D technology makes a difference, Future perspective for 3D cell culture technology and further development |
60 minutes |
| New 3D-Printable Hydrogel-Based and Particle-Based Biomaterial Ink Platforms |
Hydrogel bioprinting, 3D-Painting, organ engineering |
75 minutes |
| 3D Cell Culture Innovations |
hanging drop, encapsulation |
57 minutes |
| Cell Design Studio |
|
75 minutes |
| Modifying Cell Lines for Target Discovery and Validation |
cell line engineering |
27 minutes |
| Duolink |
Amplified Detection of Proteins and their Interactions using the Duolink® Proximity Ligation Assay
|
Proximity ligation assay, Duolink, immunofluorescence, microscopy, immunohistochemistry |
60 minutes |
Introduction to Duolink and PLA Technology
|
Duolink, PLA, Protein Interaction
|
45 minutes
|
Integrating the Proximity Ligation Assay (PLA) into your Research Program
|
Antibody, PLA, Validation
|
48 minutes
|
Molecular Annotation of EGFR Signaling-Associated Complexes in Human Cancer
|
EGFR, Cancer, Diagnostics
|
52 minutes
|
Visualizing DNA Methylation (5mC) on a Specific Genomic Locus (SEPTIN9) in Individual Human Cancer Cells usingIn-situ Hybridization and Proximity Ligation Assays
|
Duolink, Methylation, SEPTIN9
|
36 minutes
|
Duolink in Biomedical Science: A Technique for Unraveling the Molecular Mechanisms and Uncovering the Hidden Ultrastructural Features of Gap Junctions
|
PLA, Gap Junctions, Subcellular Localization
|
51 minutes
|
Detection, quantification and visualisation of protein interactions for hit profiling by high content screening
|
PLA, Duolink, Protein Interaction
|
59 minutes
|
| Genomics |
|
|
| High Protein Yields in Shaken Cultures by EnPresso® Growth System |
Bacteria, yeast, growth system, high-cell-density, scale-up, cost savings, IPTG autoinduction |
62 minutes
|
| Gene Synthesis as an Alternative to Molecular Cloning |
Gene synthesis, synthetic DNA, cost effective, protein engg, bio-based circuit, pathway design |
30 minutes
|
| RNAi |
The Future of RNAi Screening: Complementary Technologies and Advancements using CRISPR and LentiORFs
|
Screening, LentiORF, Rescue
|
50 minutes
|
| Quality Systems and Regulatory Compliance |
| Title |
Topics Covered |
Length |
| Regulatory Compliance |
Unannounced Audits: Are You Ready?
|
European Commission, Medical Manufacturing, Medical Devices
|
34 minutes |
| The Analysis Workflow of the New EPA Method 325 for Fenceline Monitorin |
Industrial – Petroleum, Oil & Gas Refineries, D & T, Environmental Laboratories - Air |
51 minutes
|
| Drug Discovery Webinar Guide |
| Title |
Topics Covered |
Length |
Late Stage Functionalization of Drug-Like Molecules
|
drug discovery, C-H functionalization, structure activity relationships
|
61 minutes |
| High-capacity drug-delivery systems for cancer therapy based on poly(2-oxazoline)s |
POx, polymer therapeutics, drug delivery, paclitaxel, hydrophobic, drug loading |
71 minutes
|
| Small-Molecule Probes and Inhibitors |
| Navigating the Promise and Perils of Chemical Probes |
Inhibitor, Chemical Probes, Protein, Signal Transduction |
47 minutes
|
| Sulfonyl Fluorides in Chemical Biology and Drug Discovery Webinar |
Chemical biology, Sulfonyl fluorides, Chemical probes, Drug discovery, Target identification |
60 minutes
|
| Analytical Webinar Guide |
| Title |
Topics Covered |
Length |
| General HPLC (High Performance Liquid Chromatography) |
HPLC Column Fundamentals
|
separation, resolution
|
28 minutes |
| HPLC Instrument Bandwidth and Optimizing Systems for High-Performance |
sample dispersion, flow rate, bandwidth |
14 minutes |
| Using LC/MS to Meet the Challenges of a High Through-put Clinical Diagnostic Lab |
LC/MS, applications, clinical diagnostics |
41 minutes |
| The Use of Alternate Selectivity in Reversed-Phase HPLC |
RP phases, separation chemistry |
43 minutes |
| A Practical 2D-LC System Developed for Complex Pharmaceuticals |
LC x LC, construction, application |
31 minutes |
| Chromogenic Media - Principle and Range of Media |
applications, methods |
8 minutes |
| Kromasil Eternity HPLC Columns - Designed for Long Life |
pH ranges, methods, applications |
17 minutes |
| Applying Best-in-class HPLC Column Technologies to the Analysis of Proteins and Biotherpeutics |
protein HPLC, peptide HPLC, biotherapeutics |
60 minutes |
| Applying Best in Class Size Exclusion and Hydrophilic Interaction Chromatography Technologies to the Analysis of Glycoproteins |
SEC-MS, HILIC-MS, Glycan Analysis |
57 minutes
|
| HILIC (Hydrophilic Interaction Liquic Chromatography) |
Using Silica HPLC Colums in HILIC Mode: A Practical Guide
|
silica, HILIC, applications, methods
|
44 minutes |
| Retention Mechanisms in HILIC: Robust Method Development |
HILIC, selectivity, method development |
55 minutes |
| Fused-Core |
HPLC Method Development and Transfer Utilizing Fused-Core Particle Technologies
|
fused-core, column efficiency
|
31 minutes |
| Ultra-Fast LC/MS/MS in Bioanalysis Using Fused-Core Columns |
high throughput, methods, applications |
39 minutes |
| HPLC of Peptides: Speed and Resolution with Fused-Core Columns |
fused-core, capacity, efficiency |
24 minutes |
| Ultra-High Performance using Fused-Core HPLC Columns |
HPLC, LC/MS, sensitivity |
28 minutes |
| Ascentis Columns |
Introducing Ascentis Express 5 um HPLC Columns with Fused-Core Technology
|
Ascentis Express, HPLC, pressure, speed, resolution
|
52 minutes |
| Introduction to Ascentis HPLC Columns |
Ascentis column, small molecule separation |
23 minutes |
| Introducing Ascentis Express F5 with Applications and Theory |
F5, methods, applications |
54 minutes |
| Introduction to Ascentis Express HPLC Columns |
fused-core, speed, methods, UHPLC |
10 minutes |
| Introducing Ascentis Express ES-Cyano HPLC Columns |
ES-Cyano, retention, selectivity |
37 minutes |
| Diagnostic Applications |
| Lung Cancer |
Lung, Cancer, Diagnostic, IHC, immunohistochemistry |
66 minutes |
| Colorectal Cancer and Immunohistochemistry |
Colorectal cancer, Diagnosed cancer, The National Cancer Institute, Immunohistochemistry, Proteins |
58 minutes |
| Multiplex Universal Peptide Quant Approach Using Microflow LC/MS/MS for Non-Human Preclinical PK Study of Therapeutic Monoclonal Antibodies |
Silumab, Therapeutics, PK Studies |
52 minutes |
Rapid LC/MS/MS-based Methods for Confirmatory Analysis of Opiates and Benzodiazepines
|
LC/MS, applications, forensics |
26 minutes |
| Removal of Phospholipids and Proteins from Plasma Using HybridSPE |
plasma, sensitivity, column life |
5 minutes |
| Breast Cancer and Immunohistochemistry |
Breast Cancer Awareness, detection, Immunohistochemistry, e-cadherin, p120 catenin, mammaglobin, and GATA3 |
59 minutes
|
| Skin Cancer and Immunohistochemistry |
Skin Cancer, Diagnosed cancer, Immunohistochemical, Skin tissue pathology, Survival rates of skin cancer |
62 minutes |
| Pediatric Cancer and IHC |
Childhood cancer, pediatric cancer |
60 minutes |
| SPME (Solid Phase MicroExtraction) |
Miniaturization of Sample Handling in Bioanalysis by SPME
|
SPME, high throughput, 96 well |
49 minutes |
| Introduction to SPME (Solid Phase MicroExtraction) |
SPME, applications |
69 minutes |
| Expanding the Capabilities of GC and MS with ionic Liquids |
complex mixtures, water quantitation, food/beverage analysis |
41 minutes |
| Miscellaneous |
Introduction to Karl Fischer Titration
|
product development, applications, water content determination
|
62 minutes |
| Introduction to Air Monitoring |
passive, active, methods, equipment |
60 minutes |
| Analysis of Contaminants & Competition: Handling R&D Roadblocks |
method development, case studies |
29 minutes |
| Evaluations of a New ionic Liquid Staionary Phase with PEG Selectivity |
GC, thermal stability |
48 minutes |
| Ionic Liquid GC Columns for the Quantification of Trans Fatty Acids in Fats and Oils |
capillary column, complex lipid separation |
31 minutes |
| The Extraction of Glycols from Water Using Supelco ENVI-Carb Plus |
glycol removal, methods |
8 minutes |
| Certified Testing Materials & Proficiency Testing in the 17025 Laboratory |
reference material, testing, proficiency |
46 minutes |
| Enzyme Explorer Navigation and Application |
web-based tool, pathway, library, substrate, inhibitor, IVD, diagnostics, genomics, drug discovery |
33 minutes |
| H&E and PAS Troubleshooting and Technical Advice |
H&E, Staining in histology, Diagnostics, Oncology |
62 minutes |
| Flow Cytometry |
| Quantitative Image Analysis and Emerging Applications on the ImageStream |
ImageStream, Flow cytometer, Fluorescence, Nuclear localization, Macrophages |
55 minutes |
| Multispectral Imaging Flow Cytometry: Illuminating T cell APC Interaction |
T cells, antigen presenting cells, Immunological synapses, ImageStream, Flow Cytometry |
56 minutes |
| New Guava and Muse Algae Kits for Flow Cytometry |
Technology description, novel methods for algal characterization, emerging applications |
39 minutes |
| Materials Science Webinar Guide |
| Title |
Topics Covered |
Length |
| Introduction to Gold Nanoparticles - Properties and Applications in Life Science |
optical and physical properties of gold nanoparticles, surface modification strategies, biomedical applications |
44 minutes |
| Graphene-Based Nanomaterials for Versatile Biosensors |
electronic and chemical properties, reduced grapheme oxide (rGO), crumpled grapheme (CG), and vertically-oriented grapheme (VG) |
61 minutes |
| Graphene and new monoatomic materials: using 2-dimensional nanosheets in a 3-dimensional world |
Graphene, supramolecular chemistry, 2D to 3D, 2D meta-materials, conductive, insulating, nanosheets |
73 minutes |
