Enzyme Explorer

Proteolytic Enzymes II

Caspase

 

Inhibitors:

 

Biotin-Val-Ala-Asp(OMe)-Fluoromethyl ketone, B2057
N-T-Boc-Asp(OMe)-fluoromethyl ketone, B2682
Z-Ala-Glu(OMe)-Val-Asp(OMe)-Fluoromethyl ketone, C8484
Z-Val-Lys(Biotin)-Asp(OMe)-Fluoromethyl ketone, C8609

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Caspase 1

Product Name
Product # 
Caspase 1 human C5482
 

Specificity:

 

Release of interleukin 1b by specific cleavage at -Asp116-Ala- and -Asp27-Gly- bonds in precursor

 

Synthethic Substrates:

 

N-Acetyl-Tyr-Val-Ala-Asp p-nitroanilide, A3831
N-Acetyl-Trp-Glu-His-Asp-p-nitroanilide, A6845
N-Acetyl-Trp-Glu-His-Asp-7-amido-4-methylcoumarin, A0216
N-Acetyl-Tyr-Val-Ala-Asp-7-amido-4-methylcoumarin, A2452
N-Acetyl-Trp-Glu-His-Asp-7-amide-4-trifluoromethylcoumarin, A6720
N-Acetyl-Tyr-Val-Ala-Asp-7-amido-4-trifluoromethylcoumarin, A9965
7-Methoxycoumarin-4-acetyl-Tyr-Val-Ala-Asp-Ala-Pro-(2,4-dinitrophenyl)Lys, M5295

 

Inhibitors:

 

N-Acetyl-Trp-Glu-His-Asp-al, A1466
N-Acetyl-Tyr-Val-Ala-Asp chloromethyl ketone, A4211
N-Acetyl-Tyr-Val-Lys(biotinyl)-Asp 2,6-dimethylbenzoyloxymethyl ketone, A4339
Z-Leu-Glu-(OMe)-Val-Asp(OMe)-Fluoromethyl ketone, C9484

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Caspase 2

Product Name
Product # 
Caspase 2 human C2854
 

Synthethic Substrates:

 

N-Acetyl-Val-Asp-Val-Ala-Asp-p-nitroanilide, A5470
N-Acetyl-Val-Asp-Val-Ala-Asp-7-amido-4-trifluoromethylcoumarin, A5345

 

Inhibitors:

 

N-Acetyl-Val-Asp-Val-Ala-Asp-CHO, A2222
N-Cbz-Val-Asp(OMe)-Val-Ala-Asp(OMe)-FMK, C1605

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Caspase 3

Product Name
Product # 
Caspase 3 human C1224
 

Specificity:

 

Cleaves PARP, ICAD, gelsolin, procaspases 6 and 9

 

Synthethic Substrates:

 

N-Acetyl-Asp-Glu-Val-Asp p-nitroanilide, A2559
N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-trifluoromethylcoumarin, A0466
Acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin, A1086
N-Acetyl-Asp-Gln-Met-Asp-7-amido-4-trifluoromethylcoumarin, A5595
Chromofluor 96, C7605
DFF45/ICAD human, D3815

 

Inhibitors:

 

N-AcetylL-Asp-Glu-Val-Asp-al, A0835
N-Acetyl-Ile-Glu-Thr-Asp-al, A1216
N-Acetyl-Glu-Ser-Met-Asp-al, A1341
Biotin-Asp-(OMe)-Glu-(OMe)Val(OMe)-Asp(OMe)-Fluoromethyl ketone, B1807
N-Cbz-Asp(OMe)-Gln-Met-Asp(OMe)-FMK, C0480
Caspase-4 Inhibitor (aldehyde), C0859
Z-VAD-FMK, V116

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Caspase 4

Product Name
Product # 
Caspase 4 human C6357
 

Synthethic Substrates:

 

N-Acetyl-Tyr-Val-Ala-Asp p-nitroanilide, A3831
N-Acetyl-Leu-Glu-Val-Asp-7-amido-4-trifluoromethylcoumarin, A2099
N-Acetyl-Tyr-Val-Ala-Asp-7-amido-4-methylcoumarin, A2452
N-Acetyl-Tyr-Val-Ala-Asp-7-amido-4-trifluoromethylcoumarin, A9965

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Caspase 5

Product Name
Product # 
Caspase 5 human C6482
 

Synthethic Substrates:

 

N-Acetyl-Trp-Glu-His-Asp-p-nitroanilide, A6845
N-Acetyl-Trp-Glu-His-Asp-7-amide-4-trifluoromethylcoumarin, A6720

 

Inhibitors:

 

N-Acetyl-Trp-Glu-His-Asp-al, A1466

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Caspase 6

Product Name
Product # 
Caspase 6 human C4977
 

Synthethic Substrates:

 

N-Acetyl-Val-Glu-Ile-Asp-p-nitroanilide, A5220
N-Acetyl-Val-Glu-Ile-Asp-7-amido-4-methylcoumarin, A0341
N-Acetyl-Val-Glu-His-Asp-7-amido-4-trifluoromethylcoumarin, A2097
N-Acetyl-Val-Glu-Ile-Asp-7-amido-4-trifluoromethylcoumarin, A5095
Caspase 6 Assay Kit, Fluorimetric, CASP-6-F

 

Inhibitors:

 

N-Acetyl-Val-Glu-Ile-Asp-al, A6339
Biotin-Asp-(OMe)-Glu-(OMe)Val(OMe)-Asp(OMe)-Fluoromethyl ketone, B1807
N-Cbz-Asp(OMe)-Gln-Met-Asp(OMe)-FMK, C0480
N-CBZ-Val-Glu-(OMe)-Ile-Asp(OMe)-FMK, C1730

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Caspase 7

Product Name
Product # 
Caspase 7 human C2979
 

Synthethic Substrates:

 

N-Acetyl-Asp-Glu-Val-Asp p-nitroanilide, A2559
N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-trifluoromethylcoumarin, A0466
Acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin, A1086

 

Inhibitors:

 

Biotin-Asp-(OMe)-Glu-(OMe)Val(OMe)-Asp(OMe)-Fluoromethyl ketone, B1807
N-Cbz-Asp(OMe)-GLu(OMe)-Val-Asp(OMe)-FMK, C0605

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Caspase 8

 

Specificity:

 

Cleaves procaspases 3,6, and 7

 

Synthethic Substrates:

 

N-Acetyl-Ile-Glu-Thr-Asp-p-nitroanilide, A9968
Caspase 8 Colorimetric Kit, CASP-8-C
N-Acetyl-Ala-Glu-Val-Asp-7-amido-4-trifluoromethylcoumarin, A0973
N-Acetyl-Ile-Glu-Thr-Asp-7-Amido-4-methylcoumarin, A4188
N-Acetyl-Leu-Glu-Thr-Asp-7-Amido-4-trifluoromethylcoumarin, A6095
Z-Ile-Glu-Thr-Asp 7-amido-4-trifluoromethylcoumarin, C5599
Chromofluor 96, C7605
Caspase 8 Fluorimetric Kit, CASP-8-F

 

Inhibitors:

 

N-Acetyl-Ile-Glu-Thr-Asp-al, A1216
Biotin-Asp-(OMe)-Glu-(OMe)Val(OMe)-Asp(OMe)-Fluoromethyl ketone, B1807
N-Cbz-Ile-Glu(OMe)-Thr-Asp(OMe), C1230
Z-Leu-Glu-(OMe)-Thr-Asp-(OMe)-Fluoromethyl ketone, C8734

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Caspase 9

Product Name
Product # 
Caspase 9 human C8726
 

Specificity:

 

Cleaves procaspases 3,6, and 7

 

Synthethic Substrates:

 

N-Acetyl-Leu-Glu-His-Asp-p-nitroaniline, A5970
N-Acetyl-Val-Glu-His-Asp-7-amido-4-trifluoromethylcoumarin trifluoroacetate salt, A2097
N-Acetyl-Leu-Glu-His-Asp-7-amido-4-trifluoromethylcoumarin, A5845
Chromofluor 96, C7605

 

Inhibitors:

 

N-Cbz-Leu-Glu(OMe)-His-Asp(OMe)-FMK, C1355

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Caspase 10

Product Name
Product # 
Caspase 10 human, recombinant, expressed in Escherichia coli >90% (SDS-PAGE) Lyophilized powder 8,000 units/mg protein C7982
Caspase 10 human, recombinant, expressed in Escherichia coli >90% (SDS-PAGE) Lyophilized powder 8,000 units/mg protein (Bradford) C6607
 

Specificity:

 

Cleaves procaspases 3, 4, 6, 7, 8 and 9

 

Inhibitors:

 

Biotin-Asp-(OMe)-Glu-(OMe)Val(OMe)-Asp(OMe)-Fluoromethyl ketone, B1807

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Caspase 13

 

Inhibitors:

 

Z-Leu-Glu(OMe)-Glu(OMe)-Asp(OMe)-Fluoromethyl ketone, C8859

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Cathepsin B, E.C. 3.4.22.1

Product Name
Product # 
Cathepsin B from bovine spleen C6286
Cathepsin B from human liver C8571
Cathepsin B from human placenta C0150
 

Specificity:

 

Hydrolysis of proteins with broad specificity for peptide bonds. Preferentially cleaves -Arg-Arg bonds in small molecule substrates (thus differing from cathepsin L). In addition to being an endopeptidase, shows peptidyl-dipeptidase activity, liberating C-terminal dipeptides

 

Synthethic Substrates:

 

Na-Benzoyl-L-arginine 4-methoxy-beta-naphthylamide hydrochloride, B0885
Z-Val-Lys-Lys-Arg-4-methoxy-beta-naphthylamide trihydrochloride, C2772
Z-Phe-Arg 4-methoxy-beta-naphthylamide hydrochloride, C3282
Z-Arg-Arg-7-amido-4-Z-RR-AMC-methylcoumarin hydrochloride, C5429
Z-Arg-Arg 4-methoxy-beta-naphthylamide acetate salt, C5520
Z-Ala-Arg-Arg 4-methoxy-beta-naphthylamide acetate salt, C8536
N-Succinyl-Leu-Leu-Val-Tyr-7-Amido-4-Methylcoumarin, S6510
Hippuryl-Arg, H2508

 

Inhibitors:

 

N-Acetyl-Leu-Leu-Met-al, A6060
N-Acetyl-Leu-Leu-Norleu-al, A6185
Antipain, A6191
Biotin-Phe-Ala-Fluoromethyl ketone, B1932
Cystatin, C0408
CA-074, C5732
CA-074 Methyl ester, C5857
Chymostatin, C7268
Cystatin, C8917
Z-Phe-Phe-Fluoromethyl ketone, C9109
E-64, E3132
Leupeptin, L2023
MeOSuc-Phe-homo-Phe fluoromethyl ketone, M5314
a2-Macroglobulin, M6159
Procathepsin B, rat, fragment 26-50, P0350
Procathepsin B, rat, fragment 36-50, P0475

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Cathepsin C, E.C. 3.4.14.1

Product Name
Product # 
Cathepsin C from bovine spleen C8511
 

Specificity:

 

Release of an N-terminal dipeptide, Xaa-Xbb Xcc-, except when Xaa is Arg or Lys, or Xbb or Xcc is Pro

 

Synthethic Substrates:

 

Gly-Phe p-nitroanilide, G0142
Gly-Phe beta-naphthylamide, G4255
Gly-Phe beta-naphthylamide, G9512
Ser-Tyr beta-naphthylamide, S5628

 

Inhibitors:

 

Iodoacetic Acid, I2512

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Cathepsin D, E.C. 3.4.23.5

Product Name
Product # 
Cathepsin D from bovine spleen C3138
Cathepsin D from human liver C8696
 

Specificity:

 

Specificity similar to, but narrower than, that of pepsin A. Does not cleave the Gln4-His bond in B chain of insulin

 

Synthethic Substrates:

 

Boc-Phe-Ala-Ala-p-Nitro-Phe-Phe-Val-Leu 4-hydroxymethylpyridine ester, B1644
N-Acetyl-Arg-Gly-Phe-Phe-Pro-7-amido-4-methylcoumarin, C6358
N-Acetyl-Arg-Gly-Phe-Phe-Pro-7-amido-4-trifluoromethylcoumarin, C6983
N-Acetyl-Arg-Gly-Phe-Phe-Pro 4-methoxy-2-naphthylamide, C7108
7-Methoxycoumarin-4-Acetyl-Gly-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Lys(DNP)-D-Arg-amide, M0938

 

Inhibitors:

 

Antipain, A6191
Chymostatin, C7268
Pepstatin A, P5318

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Cathepsin G, E.C. 3.4.21.20

Product Name
Product # 
Cathepsin G from human leukocytes C4428
Cathepsin G from human sputum C5108
 

Specificity:

 

Specificity similar to chymotrypsin C

 

Synthethic Substrates:

 

N-Methoxysuccinyl-Ala-Ala-Pro-Met p-nitroanilide, M7771
N-Succinyl-Ala-Ala-Pro-Phe p-nitroanilide, S7388
N-Succinyl-Gly-Gly-Phe-7-amido-4-trifluoromethylcoumarin, S4689
N-Succinyl-Gly-Gly-Phe-4-methoxy-2-naphthylamide, S4814
N-Succinyl-Ala-Ala-Pro-Phe thiobenzyl ester, S6518
N-Succinyl-Phe-Leu-Phe thiobenzyl ester, S6643

 

Inhibitors:

 

a1-Antchymotrypsin, A9285
Chymostatin, C7268
Z-Gly-Leu-Phe-Chloromethyl ketone, C9984
Diisopropyl fluorophosphate, D0879
3,4-Dichloroisocoumarin, D7910
N-Acetyl-eglin C, E7888

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Cathepsin H, E.C. 3.4.22.16

Product Name
Product # 
Cathepsin H from human liver C8821
 

Specificity:

 

Hydrolysis of proteins, acting as an aminopeptidase (notably, cleaving Arg bonds) as well as an endopeptidase

 

Synthethic Substrates:

 

L-Arginine-7-amido-4-methylcoumarin hydrochloride, A2027

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Cathepsin L, E.C. 3.4.22.15

Product Name
Product # 
Cathepsin L from human liver C6854
 

Specificity:

 

Similar to that of papain. As compared to cathepsin B, cathepsin L exhibits higher activity towards protein substrates and no peptidyl-dipeptidase activity

 

Synthethic Substrates:

 

Z-Phe-Arg 4-methoxy-beta-naphthylamide hydrochloride, C3282

 

Inhibitors:

 

N-Acetyl-Leu-Leu-Norleu-al, A6185

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Chymopapain, E.C. 3.4.22.6

Product Name
Product # 
Chymopapain from papaya latex C8526
 

Specificity:

 

Similar to that of papain

 

Synthethic Substrates:

 

BAEE, B4500

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