Enzyme Explorer

Proteolytic Enzymes III

Chymase, E.C. 3.4.21.39

Product Name
Product # 
Chymase C9612
 

Specificity:

 

Preferential cleavage: Phe-Xaa > Tyr-Xaa > Trp-Xaa > Leu-Xaa

 

Synthethic Substrates:

 

BTEE, B6125

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Chymotrypsin, a-, E.C. 3.4.21.1

Product Name
Product # 
a-Chymotrypsin from bovine pancreas, suitable for protein sequencing Salt-free, lyophilized powder C6423
b-Chymotrypsin from bovine pancreas C4629
a-Chymotrypsin Insoluble enzyme attached to carboxymethyl cellulose from bovine pancreas C7260
a-Chymotrypsin-Agarose from bovine pancreas C9134
a-Chymotrypsin DFP treated C1012
a-Chymotrypsin from human pancreas, Lyophilized powder C8946
a-Chymotrypsin from bovine pancreas, Type II Essentially salt-free, lyophilized powder >40 units/mg protein C4129
a-Chymotrypsin from bovine pancreas, Type I-S Essentially salt-free, lyophilized powder 40-60 units/mg protein C7762
a-Chymotrypsin TLCK Treated from bovine pancreas C3142
 

Specificity:

 

Preferential cleavage: Ty-Xaa, Trp-Xaa, Phe-Xaa, Leu-Xaa

 

Synthethic Substrates:

 

N-Succinyl-Ala-Ala-Pro-Phe p-nitroanilide, S7388
N-Succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin, S9761
Ala-Ala-Phe-7-amido-4-methylcoumarin, A3401
ALA-ALA-VAL-ALA P-Nitroanilide, A9273
BTEE, B6125
N-Benzoyl-L-tyrosine p-nitroanilide , B6760
Glutaryl-Ala-Ala-Phe-4-methoxy-b-naphthylamide, G3769
N-Glutaryl-Gly-Gly-Phe-b-naphthylamide, G4636
4-Methylumbelliferyl p-trimethylammoniocinnamate chloride, M4507
N-Succinyl-Gly-Gly-Phe p-nitroanilide, S1899
N-Succinyl-L-phenylalanine p-nitroanilide, S2628
N-Succinyl-Ala-Ala-Pro-Phe 7-amido-4-trifluoromethylcoumarin, S5189
N-Succinyl-Ala-Ala-Pro-Phe 4-methoxy-2-naphthylamide, S5314

 

Inhibitors:

 

Z-L-Phe chloromethyl ketone, C9511
a2-Antiplasmin, A0914
Aprotinin, A1153
e-Amino-n-caproic acid, A2504
a1-Antitrypsin, A6150
e-Amino-n-caproic acid, A7824
4-(2-Aminoethyl) benzenesulfonyl fluoride hydrochloride, A8456
a1-Antitrypsin, A9024
a1-Antchymotrypsin, A9285
Bromoenol lactone, B1552
Chymostatin, C7268
Diisopropyl fluorophosphate, D0879
Ecotin, E1398
N-Acetyl-eglin C, E7888
Gabexate mesylate, G2417
Leupeptin, L2023
Trypsin Inhibitor, T0256
Trypsin Inhibitor, T1886
N-Tosyl-L-phenylalanine chloromethyl ketone, T4376
Trypsin Inhibitor, T4385
Trypsin Inhibitor, T9253
Trypsin Inhibitor, T9378
Trypsin-chymotrypsin inhibitor, T9777

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Clostripain, E.C. 3.4.22.8

Product Name
Product # 
Clostripain from Clostridium histolyticum C0888
 

Specificity:

 

Preferential cleavage: Arg-Xaa , including Arg-Pro, but not Lys-

 

Synthethic Substrates:

 

BAEE, B4500

 

Inhibitors:

 

p-Chloromercuribenzoic acid Free Acid, C5913
Histatin 5, H6027
N a-p-Tosyl-L-lysine chloromethyl ketone hydrochloride, T7254

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Collagenase, E.C. 3.4.24.3

Product Name
Product # 
Collagenase from Clostridium histolyticum, 2-5 FALGPA units/mg solid >1200 CDU/mg solid (CDU = collagen digestion units) Type XI C7657
Collagenase from Clostridium histolyticumType VII powder 1000-3000 CDU/mg solid (CDU = collagen digestion units) 4-12 FALGPA units/mg protein cell culture, tested C2799
Collagenase from Clostridium histolyticum, cell culture, tested Lyophilized powder Type XI-S C9697
Collagenase from Clostridium histolyticum, Type XI powder >1200 CDU/mg solid (CDU = collagen digestion units) 2-5 FALGPA units/mg protein cell culture, tested C9407
Collagenase/Dispase from Achromobacter iophagus/Bacillus polymyxa C3180
Collagenase from Clostridium histolyticum, Type I-A powder >125 CDU/mg solid (CDU = collagen digestion units) 0.5-2.0 FALGPA units/mg protein cell culture, tested C2674
Collagenase from Clostridium histolyticum, Type VII-S cell culture, tested Lyophilized powder C9572
Collagenase from Clostridium histolyticum, Type III Fraction A Lyophilized powder 2-10 FALGPA units/mg protein minimum 400 CDU/mg solid (CDU = collagen digestion units) C0255
Collagenase from Clostridium histolyticum, Type VII Lyophilized powder 1,000-3,000 CDU/mg solid (CDU = collagen digestion units) 4-12 FALGPA units/mg protein C0773
Collagenase from Clostridium histolyticum, 1.1-1.5 FALGPA units/mg protein (Neutral Protease: 20-50units per mg solid) Sigma Blend Type H C8051
Collagenase from Clostridium histolyticum, 1-3 FALGPA units/mg protein >125 CDU/mg solid (CDU = collagen digestion units) Type V C9263
Collagenase from Clostridium histolyticum, 1.8-2.2 FALGPA units/mg protein (Neutral Protease:<10 units per mg solid) Sigma Blend Type F C7926
Collagenase from Clostridium histolyticum, 0.5-0.9 FALGPA units/mg protein (Neutral Protease: 50-80 units per mg solid) Sigma Blend Type L C8176
Collagenase + protease inhibitor, suitable (Each lot is evaluated for suitability in the release of physiologically active rat pancreatic islets (for methodology see Type V)) 2-5 FALGPA units/mg protein minimum C6079
Collagenase from Clostridium histolyticum, Essentially salt-free, lyophilized powder 0.5-3.0 FALGPA units/mg protein >125 CDU/mg solid (CDU = collagen digestion units) Type IA C9891
Collagenase from Clostridium histolyticum, Essentially salt-free, lyophilized powder 0.25-1.0 FALGPA units/mg protein >125 CDU/mg solid (CDU = collagen digestion units) Type I C0130
Collagenase from Clostridium histolyticum, suitable for release of rat epididymal adipocytes and hepatocytes (for methodology see Type II and Type IV) 0.5-3.0 FALGPA units/mg solid >125 CDU/mg solid (CDU = collagen digestion units) Type VIII C2139
Collagenase from Clostridium histolyticum, 0.5-3.0 FALGPA units/mg solid >125 CDU/mg solid (CDU = collagen digestion units) Type II C6885
Collagenase from Clostridium histolyticum, 0.5-3.0 FALGPA units/mg solid >125 CDU/mg solid (CDU = collagen digestion units) Type IV C5138
Collagenase from Clostridium histolyticum, Type I-AS cell culture, tested Lyophilized powder C9722
 

Specificity:

 

Digestion of native collagen in the triple helical region at Gly bonds. With synthetic peptides, a preference is shown for Gly at P3 and P1'; Pro and Ala at P2 and P2'; and hydroxyproline, Ala or Arg at P3'

 

Synthethic Substrates:

 

Z-Gly-Pro-Leu-Gly-Pro, C0627
Collagen Type I-FITC, C4361
Collagen Type II-FITC, C4486
Collagen-Fluorescein, C5858
Collagen-Fluorescein, C8008
N-(2,4-Dinitrophenyl)-Pro-Leu-Gly-Cys(Me)-His-Ala-D-Arg amide, D0296
FALGPA, F5135

 

Inhibitors:

 

5-(R,S)-T-trans-Cinnamido-7-methyl-4-oxo-octanoyl-L-prolyl-L-proline, C0360
Dichloromethylenediphosphonic acid disodium salt, D4434
Ecotin, E1398
EDTA, ED2SS
Isoamylphosphonyl-Gly-L-Pro-L-Ala, dipotassium, I7029
(2R)-2-Mercaptomethyl-4-methylpentanoyl-beta-(2-naphthyl)-Ala-Ala Amide, M3531

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Complement C1r, E.C. 3.4.21.41

Product Name
Product # 
Complement C1r from human serum C2162
 

Specificity:

 

Selective cleavage of Lys(or Arg) Ile bond in complement subcomponent C1s

 

Inhibitors:

 

Complement C1 esterase inhibitor, C2412
Diisopropyl fluorophosphate, D0879
C1 Esterase inhibitor, E0518

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Complement C1s, E.C. 3.4.21.42

 

Specificity:

 

Cleavage of Arg-Ala bond in complement component C4 to form C4a and C4b, and Lys(or Arg) Lys bond in complement component C2 to form C2a and C2b: the "classical" pathway C3 convertase

 

Inhibitors:

 

Complement C1 esterase inhibitor, C2412
Diisopropyl fluorophosphate, D0879
C1 Esterase inhibitor, E0518

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Complement Factor D, E.C. 3.4.21.46

Product Name
Product # 
Complement factor D from human plasma C5688
 

Specificity:

 

Selective cleavage of Arg-Lys bond in complement factor B when in complex with complement subcomponent C3b or with cobra venom factor

 

Inhibitors:

 

Diisopropyl fluorophosphate, D0879

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Complement factor I, E.C. 3.4.21.45

Product Name
Product # 
Complement factor I from human plasma C5938
 

Specificity:

 

Inactivates complement subcomponents C3b, iC3b and C4b by proteolytic cleavage

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Cucumisin, E.C. 3.4.21.25

Product Name
Product # 
Cucumisin C1932
 

Specificity:

 

Hydrolysis of proteins with broad specificity

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Dipeptidyl peptidase IV, E.C. 3.4.14.5

Product Name
Product # 
Dipeptidyl Peptidase from porcine kidney D7052
 

Specificity:

 

Release of an N-terminal dipeptide, Xaa-Xbb Xcc-, from a polypeptide, preferentially when Xbb is Pro, provided Xcc is neither Pro nor hydroxyproline

 

Synthethic Substrates:

 

Gly-Pro-7-amido-4-methylcoumarin hydrobromide, G2761
Gly-Pro p-nitroanilide hydrochloride, G0513

 

Inhibitors:

 

Ile-Pro-Ile, I9759

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Elastase, leukocyte, E.C. 3.4.21.37

Product Name
Product # 
Elastase from human leukocytes E8140
 

Specificity:

 

Hydrolysis of proteins, including elastin. Preferential cleavage Val-Xaa > Ala-Xaa

 

Synthethic Substrates:

 

Boc-Ala-Pro-Nva p-chlorothiobenzyl ester, B2017
N-Methoxysuccinyl-Ala-Ala-Pro-Val-7-amido-4-trifluoromethylcoumar, M4563
N-(Methoxysuccinyl)-Ala-Ala-Pro-Val 4-nitroanilide, M4765
N-Methoxysuccinyl-Ala-Ala-Pro-Val-7-amido-4-methylcoumarin, M9771

 

Inhibitors:

 

Aprotinin, A1153
Diisopropyl fluorophosphate, D0879
3,4-Dichloroisocoumarin, D7910
Eglin C fragment 60-63 methyl ester, E2017
N-Acetyl-eglin C, E7888
a2-Macroglobulin, M6159
Phenylmethylsulfonyl fluoride, P7626

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Elastase, pancreatic, E.C. 3.4.21.36

Product Name
Product # 
Elastase, pancreatic solution from porcine pancreas, Type I Aqueous suspension minimum 4 units/mg protein E1250
Elastase, pancreatic from porcine pancreas, Lyophilized powder 3-6 units/mg protein (biuret) cell culture, tested E7885
Elastase, pancreatic from porcine pancreas, Type IV Lyophilized powder minimum 4 units/mg protein (biuret) E0258
Elastase, pancreatic from porcine pancreas, Type II-A Lyophilized powder minimum 1 units/mg protein (biuret) E6883
Elastase, pancreatic from porcine pancreas, Type III Lyophilized powder minimum 4 units/mg protein E0127
 

Specificity:

 

Hydrolysis of proteins, including elastin. Preferential cleavage: Ala-Xaa

 

Synthethic Substrates:

 

N-Methoxysuccinyl-Ala-Ala-Pro-Val-7-amido-4-methylcoumarin, M9771

 

Inhibitors:

 

a1-Antitrypsin, A6150
a1-Antitrypsin, A9024
Diisopropyl fluorophosphate, D0879
3,4-Dichloroisocoumarin, D7910
Ecotin, E1398
a2-Macroglobulin, M6159

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Endoproteinase Arg-C, E.C. 3.4.21.35

Product Name
Product # 
Endoproteinase Arg-C murine submaxillary gland, suitable for protein sequencing Lyophilized powder P6056
Endoproteinase Arg-C murine submaxillary gland, Type XX-S Lyophilized powder 250-800 units/mg protein (Lowry) P5171
 

Specificity:

 

Cleaves specifically at the carboxyl side of arginine residues. The enzyme has also been shown to cleave Lys-Lys and Lys-Arg bonds; all Arg-X bonds may not be hydrolyzed. This enzyme also exhibits esterase and amidase activities.

 

Synthethic Substrates:

 

TAME, T4626

 

Inhibitors:

 

Diisopropyl fluorophosphate, D0879
a2-Macroglobulin, M6159
N-a-p-Tosyl-L-lysine chloromethyl ketone Hydrochloride, T7254

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Endoproteinase Asp-N, E.C. 3.4.24.33

Product Name
Product # 
Endoproteinase Asp-N from Pseudomonas fragi mutant strain P3303
 

Specificity:

 

Cleavage of Xaa-Asp, Xaa-Glu and Xaa-cysteic acid bonds

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Endoproteinase Glu-C, E.C. 3.4.21.19

Product Name
Product # 
Endoproteinase Glu-C Staphylococcus aureus V8, suitable for protein sequencing Lyophilized powder P6181
Endoproteinase Glu-C Staphylococcus aureus V8, Type XVII-B Lyophilized powder 500-1,000 units/mg solid P2922
 

Specificity:

 

Preferential cleavage: Glu-Xaa, Asp-Xaa

 

Inhibitors:

 

Diisopropyl fluorophosphate, D0879
a2-Macroglobulin, M6159

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Endoproteinase Lys-C, E.C. 3.4.21.50

Product Name
Product # 
Endoproteinase Lys-C from Lysobacter enzymogenes, suitable for protein sequencing Lyophilized powder P3428
Endoproteinase Lys-C from Lysobacter enzymogenes, Lyophilized powder P2289
 

Specificity:

 

Cleaves at the carboxyl side of lysyl residues. Lys-Pro and Lys-Glu bonds are also cleaved

 

Synthethic Substrates:

 

N-(p-Tosyl)-Gly-Pro-Lys 4-nitroanilide acetate salt, T6140

 

Inhibitors:

 

Aprotinin, A1153
Leupeptin, L2023
Diisopropyl fluorophosphate, D0879
N-a-p-Tosyl-L-lysine chloromethyl ketone Hydrochloride, T7254

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Enterokinase, E.C. 3.4.21.9

Product Name
Product # 
Enterokinase from bovine intestine, powder E5144
Enterokinase porcine intestine, Lyophilized powder 100-500 units/mg protein E0885
Enterokinase from bovine intestine, Salt-free, lyophilized powder >0.5 units/mg solid E5510
Enterokinase porcine intestine, Salt-free, lyophilized powder approx. 2 units/mg solid E0632
Enterokinase solution from calf intestine, Buffered aqueous glycerol solution minimum 1,000 units/mg protein (BCA) E0766
 

Specificity:

 

Activation of trypsinogen by selective cleavage of Lys6-Ile bond

 

Synthethic Substrates:

 

Gly-Asp-Asp-Asp-Asp-Lys-b-naphthylamide, G5261

 

Inhibitors:

 

Diisopropyl fluorophosphate, D0879
a2-Macroglobulin, M6159

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Factor Xa, E.C. 3.4.21.6

Product Name
Product # 
Factor X Activated (Xa) bovine plasma F2027
 

Specificity:

 

Selective cleavage of Arg-Thr and then Arg-Ile bonds in prothrombin to form thrombin

 

Synthethic Substrates:

 

N-Benzoyl-Ile-Glu-Gly-Arg p-nitroanilide hydrochloride, B2291
N-Benzoyl-Ile-Glu-Gly-Arg p-nitroanilide acetate salt, B7020
Boc-Ile-Glu-Gly-Arg-7-amido-4-methylcoumarin hydrochloride, B9760
Factor Xa chromogenic substrate, F3301
4-Methylumbelliferyl 4-guanidinobenzoate hydrochloride monohydrate, M4149

 

Inhibitors:

 

a2-Antiplasmin, A0914
Antithrombin III, Human, A2221
Antithrombin III, Rat, A6397
Antithrombin III, Bovine, A9141
Diisopropyl fluorophosphate, D0879
3,4-Dichloroisocoumarin, D7910
Ecotin, E1398
Phenylmethylsulfonyl fluoride, P7626
Trypsin Inhibitor, T9003

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