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Enzyme Explorer

Proteolytic Enzymes I

Achromopeptidase, E.C. 3.4.21.50

Product Name
Product # 
Achromopeptidase from Achromobacter lyticus
A3547
Achromopeptidase from Achromobacter lyticus, Crude powder, 300-600 units/mg solid
A7550
Achromopeptidase from Achromobacter lyticus, Partially purified powder, 20,000-40,000 units/mg solid
A3422
 

Specificity:

 

Preferential cleavage: Lys-Xaa, including -Lys-Pro-

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Aminopeptidase, E.C. 3.4.11.10

Product Name
Product # 
Aminopeptidase from Aeromonas proteolytica
A8200
 

Inhibitors:

 

2-Mercaptoethylamine HCl, M6500

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Ancrod, E.C. 3.4.21.74

Product Name
Product # 
Ancrod from Agkistrodon rhodostoma (Malayan pit viper) venom
A5042

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Angiotensin Converting Enzyme, E.C.3.4.15.1

Product Name
Product # 
Angiotensin Converting Enzyme from porcine kidney, Lyophilized powder, minimum 10 units/mg protein (Bradford)
A2580
Angiotensin Converting Enzyme from rabbit lung, Lyophilized powder, 2-6 units/mg protein (modified Warburg-Christian)
A6778
 

Specificity:

 

Conversion of angiotensin I to angiotensin II

 

Synthethic Substrates:

 

N-[3-(2-Furyl)acryloyl]-Phe-Gly-Gly, F7131
N-Hippuryl-His-Leu tetrahydrate, H1635
Hippuryl-Gly-Gly, H1763
Hippuryl-His-Leu acetate salt, H4884

 

Inhibitors:

 

Angiotensin-converting enzyme inhibitor, A0773
Bradykinin potentiator B, B0507
Bradykinin potentiator C, B0632
Captopril, C4042
Captopril USP, C8856
Foroxymithine, F5017
Fenoldopam, F6800
N-Succinyl-L-proline, S6633
N-e-Trifluoroacetyl-Lys-Pro, T7025

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Bromelain, E.C. 3.4.22.32

Product Name
Product # 
Bromelain pineapple stem, Lyophilized powder 5-10 units/mg protein B5144
Bromelain pineapple stem, 3-7 units/mg protein B4882
 

Specificity:

 

Broad specificity for cleavage of proteins, but strong preference for Z-Arg-Arg NHMec in peptides

 

Synthethic Substrates:

 

N-a-CBZ-L-Lysine-p-Nitrophenyl ester, C3637

 

Inhibitors:

 

E-64, E3132
N-Ethylmaleimide, E3876
Iodoacetic Acid, I2512
N-a-p-Tosyl-L-lysine chloromethyl ketone Hydrochloride, T7254
N-a-p-Tosyl-L-phenylalanine chloromethyl ketone Hydrochloride, T4376

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Calpain

 

Inhibitors:

 

Antipain, A6191
Acetyl-Calpastatin (184-210) Human, C4285
p-Chloromercurbenzoic acid Free Acid, C5913
Z-Leu-Leu-Leu-Fluoromethyl ketone, C8984
Z-Leu-Leu-Tyr-Fluoromethyl ketone, C9234
Z-Leu-Leu-Tyr-CHN2, C9859
(2S,3S)-trans-Epoxysuccinyl-/L-leucylamido-3-methylbutane, E0514
N-Ethylmaleimide, E3876
(2S,3S)-trans-Epoxysuccinyl-L-leucylamido-3-methylbutane ethyl ester, E8640
Leupeptin , L2023
MeOSuc-Val-homo-Phe-fluoromethyl ketone, M5439
a2-Macroglobulin, M6159

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Calpain I, E.C. 3.4.22.52

Product Name
Product # 
Calpain 1 Human C6108
 

Specificity:

 

Broad endopeptidase specificity

 

Synthethic Substrates:

 

N-Succinyl-Leu-Tyr 7-amido-4-methylcoumarin, S1153

 

Inhibitors:

 

N-Acetyl-Leu-Leu-Met-al, A6060
N-Acetyl-Leu-Leu-Norleu-al, A6185
Calpain Inhibitor Peptide, C9181

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Calpain II, E.C. 3.4.22.32

Product Name
Product # 
Calpain II from rabbit skeletal muscle P4533
 

Specificity:

 

Preferential cleavage where P1', P2' and P3' are hydrophobic residues

 

Synthethic Substrates:

 

N-Succinyl-Leu-Tyr 7-amido-4-methylcoumarin, S1153
N-Succinyl-Leu-Leu-Val-Tyr-7-Amido-4-Methylcoumarin, S6510

 

Inhibitors:

 

N-Acetyl-Leu-Leu-Met-al, A6060
Acetyl-Calpastatin (184-210) Human, C4285
p-Chloromercuribenzoic acid Free Acid, C5913
Calpain Inhibitor Peptide, C9181
(2S,3S)-trans-Epoxysuccinyl-/L-leucylamido-3-methylbutane, E0514
N-Ethylmaleimide, E3876
Leupeptin, L2023
a2-Macroglobulin, M6159

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Carboxypeptidase A, E.C. 3.4.22.17

Product Name
Product # 
Carboxypeptidase A-Agarose
C1261
Carboxypeptidase A bovine pancreas, Type I Aqueous suspension approx. 50 units/mg protein
C0261
Carboxypeptidase A, PMSF treated bovine pancreas
C9268
Carboxypeptidase A bovine pancreas, Aqueous suspension approx. 50 units/mg protein
C0386
 

Specificity:

 

Release of a C-terminal amino acid, but little or no action with -Asp, -Glu, -Arg, -Lys or -Pro

 

Synthethic Substrates:

 

Hippuryl-L-phenylalanine, H6875

 

Inhibitors:

 

Carboxypeptidase inhibitor, C0279
Diethylenetriaminepentaacetic acid Free Acid, D6518
EDTA, ED2SS
EGTA, E4378
1,10-Phenanthroline Monohydrate, P9375

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Carboxypeptidase B, E.C. 3.4.17.2

Product Name
Product # 
Carboxypeptidase B from human pancreas C5233
 

Specificity:

 

Preferential release of a C-terminal lysine or arginine amino acid

 

Synthethic Substrates:

 

Hippuryl-L-Lys, H6750
Hippuryl-L-Arg, H2508

 

Inhibitors:

 

Carboxypeptidase inhibitor, C0279
Diethylenetriaminepentaacetic acid Free Acid, D6518
EDTA, ED2SS
EGTA, E4378
1,10-Phenanthroline Monohydrate, P9375

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Carboxypeptidase G, E.C. 3.4.19.9

Product Name
Product # 
Carboxypeptidase G from Pseudomonas sp. C9658
 

Specificity:

 

Hydrolysis of a gamma-glutamyl bond

 

Synthethic Substrates:

 

(+)-Amethopterin, A6770

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Carboxypeptidase P, E.C. 3.4.17.16

Product Name
Product # 
Carboxypeptidase P from Penicillium janthinellum C5396
 

Specificity:

 

Release of a C-terminal residue other than proline, by preferential cleavage of a prolyl bond

 

Synthethic Substrates:

 

N-CBZ-Glu-Tyr, C0257

 

Inhibitors:

 

Diisopropyl fluorophosphate, D0879
Iodoacetic Acid, I2512

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Carboxypeptidase W, E.C. 3.4.16.1

Product Name
Product # 
Carboxypeptidase W from wheat C6527
 

Synthethic Substrates:

 

N-CBZ-Phe-L-Ala, C1634

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Carboxypeptidase Y, E.C. 3.4.16.5

Product Name
Product # 
Carboxypeptidase Y from Baker's yeast (S. cerevisiae), Lyophilized powder minimum 50 units/mg protein C3888
Carboxypeptidase Y from Baker's yeast (S. cerevisiae), Lyophilized powder C4046
 

Specificity:

 

Release of a C-terminal amino acid with broad specificity

 

Synthethic Substrates:

 

N-CBZ-Phe-L-Ala, C1634

 

Inhibitors:

 

Aprotinin, A1153
Diisopropyl fluorophosphate, D0879
Phenylmethylsulfonyl fluoride, P7626

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