PolyAmino Acids: Storage & Handling FAQ

  1. How should polylysine HBr or HCl be handled?
  2. What are the normal storage conditions for polyamino acids?
  3. What are the normal handling conditions for polyamino acids?
  4. If a hygroscopic polyamino acid gets exposed to moisture, how can the product be recovered?
  5. Are side chain amino groups of polylysine/polyarginine/polyornithine or side chain carboxyl groups from polyglutamic/polyaspartic acid available for conjugation/coupling

How should polylysine HBr or HCl be handled?
Polylysine HBr or HCl polymers (all chiral forms) are very hygroscopic. Avoid exposing these polymers to the atmosphere. Ideally polylysine HCl or HBr should be opened and weighed at room temperature in a dry room with 30% relative humidity or less.

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What are the normal storage conditions for polyamino acids?
Sigma-Aldrich recommends that all the polyamino acids be stored at -20°C. The product should come to room temperature before opening the container for weighing or handling in dry room with 30% humidity level or less. Products generally do not degrade if kept at recommended storage conditions.

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What are the normal handling conditions for polyamino acids?
All water-soluble polyamino acids (polylysine, polyornithine, polyglutamic acid, polyaspartic acid, and poly-DL-alanine) are hygroscopic and should be weighed/handled at room temperature in a dry room with the humidity level of 30% or less. All the hydrophobic polyamino acids should be weighed or handled at room temperature.

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If a hygroscopic polyamino acid gets exposed to moisture, how can the product be recovered?
Add water to the vial to dissolve the product and then lyophilize (freeze dry) to recover the solids.

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Are side chain amino groups of polylysine/polyarginine/polyornithine or side chain carboxyl groups from polyglutamic/polyaspartic acid available for conjugation/coupling?
Yes. Side chain amino groups of polylysine/polyarginine/polyornithine and side chain carboxyl groups of polyglutamic/polyaspartic acids are free and can be used for conjugation or coupling.

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