Figure 2. ß-gal Assay Normalized to Total Cell Protein

CHO-K1 cells were seeded in a 6-well plate. Cells were transfected with 2 µg per well of DNA using Sigma's Escort II reagent and grown in DMEM supplemented with 10% FBS plus 4 mM L-glutamine. G-418 was introduced 48 hrs post transfection at 1 mg per mL. During selection, ß-gal and BCA assays were performed at day 28 using Sigma reagents.

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