DNA & RNA Purification

Extract-N-Amp™



Your shortcut for PCR

Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays. Eliminate the need for any columns or long enzymatic sample purification with this simple “lyse & go” method for PCR. Kits include all reagents, proprietary buffers, plus enzymes needed to rapidly extract and amplify targets of interest from a variety of cells and tissues.
  • Integrated purification and amplification
  • Simplified sample processing

Watch the full video protocol here

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The kits provide all the reagents necessary to rapidly extract and amplify DNA targets of interest by PCR. A novel extraction method eliminates the need for long enzymatic digestions or homogenization. The kit also includes a specially formulated hot start PCR ReadyMix™ for amplification directly from the extract. The PCR ReadyMix comes in two formulations: Extract-N-Amp PCR ReadyMix and REDExtract-N-Amp PCR ReadyMix. The REDExtract-N-Amp PCR ReadyMix contains an inert dye that acts as a tracking dye and allows for convenient loading of PCR reaction onto agarose gels for analysis.

Sigma provides validated protocols to extract and amplify genomic DNA from animal and plant samples.

In a typical procedure, genomic DNA is extracted from a sample that has been incubated in preparation solution and extraction solution at room temperature. The sample is heated and then mixed with a third solution to neutralize inhibitory substances prior to PCR. A portion of the DNA extract is then added to a PCR reaction containing primers and the ReadyMix included in the kit. The remainder of the DNA can be refrigerated for convenient storage.

Typical Applications include:

  • Genotyping
  • Transgene detection
  • Knockout analysis
  • Sequencing
Product No. Description # of Extractions & Amplifications Starting Amount Time Required
Tissue
XNAT-10RXN REDExtract-N-Amp™ Tissue PCR Kit 10 extractions,
10 amplifications
Varies depending on starting material <15 min from extraction to amplification
XNAT-100RXN REDExtract-N-Amp™ Tissue PCR Kit 100 extractions,
100 amplifications
Varies depending on starting material <15 min from extraction to amplification
XNAT-1000RXN REDExtract-N-Amp™ Tissue PCR Kit 1000 extractions,
1000 amplifications
Varies depending on starting material <15 min from extraction to amplification
         
XNAT2 Extract-N-Amp™ Tissue PCR Kit 100 extractions,
100 amplifications
Varies depending on starting material <15 min from extraction to amplification
XNAT2R Extract-N-Amp™ Tissue PCR Kit 1000 extractions,
1000 amplifications
Varies depending on starting material <15 min from extraction to amplification
         
XNATG SYBR® Green Extract-N-Amp™ Tissue PCR Kit 100 extractions,
100 amplifications
Varies depending on starting material <15 min from extraction to amplification
XNATRG SYBR® Green Extract-N-Amp™ Tissue PCR Kit 1000 extractions,
1000 amplifications
Varies depending on starting material <15 min from extraction to amplification
         
Blood
XNAB2 Extract-N-Amp™ Blood PCR Kit 100 extractions,
100 amplifications
10 µL of blood or blood card 8 min from extraction to amplification
XNAB2R Extract-N-Amp™ Blood PCR Kit 1000 extractions,
1000 amplifications
10 µL of blood or blood card 8 min from extraction to amplification
XNAB2RE Extract-N-Amp™ Blood PCR Kit 1000 extractions,
5000 amplifications
10 µL of blood or blood card 8 min from extraction to amplification
         
XNABS REDExtract-N-Amp™ Blood PCR Kit 10 extractions,
10 amplifications
10 µL of blood or blood card 8 min from extraction to amplification
XNAB REDExtract-N-Amp™ Blood PCR Kit 100 extractions,
100 amplifications
10 µL of blood or blood card 8 min from extraction to amplification
XNABE REDExtract-N-Amp™ Blood PCR Kit 100 extractions,
500 amplifications
10 µL of blood or blood card 8 min from extraction to amplification
         
Plant
XNAP2 Extract-N-Amp™ Plant PCR Kit 100 extractions,
100 amplifications
0.5 – 0.7 cm plant leaf disk <15 min from extraction to amplification
XNAP2E Extract-N-Amp™ Plant PCR Kit 100 extractions,
500 amplifications
0.5 – 0.7 cm plant leaf disk <15 min from extraction to amplification
XNAR Extract-N-Amp™ Plant PCR Kit 1000 extractions,
1000 amplifications
0.5 – 0.7 cm plant leaf disk <15 min from extraction to amplification
         
XNAPS REDExtract-N-Amp™ Plant PCR Kit 10 extractions,
10 amplifications
0.5 – 0.7 cm plant leaf disk <15 min from extraction to amplification
XNAP REDExtract-N-Amp™ Plant PCR Kit 100 extractions,
100 amplifications
0.5 – 0.7 cm plant leaf disk <15 min from extraction to amplification
XNAPE REDExtract-N-Amp™ Plant PCR Kit 100 extractions,
500 amplifications
0.5 – 0.7 cm plant leaf disk <15 min from extraction to amplification
XNAPR REDExtract-N-Amp™ Plant PCR Kit 1000 extractions,
1000 amplifications
0.5 – 0.7 cm plant leaf disk <15 min from extraction to amplification
         
XNAPG SYBR® Green Extract-N-Amp™ Plant PCR Kit 100 extractions,
100 amplifications
0.5 – 0.7 cm plant leaf disk <15 min from extraction to amplification
         
Seed
XNAS2 Extract-N-Amp™ Seed PCR Kit 100 extractions,
100 amplifications
1 seed <15 min from extraction to amplification
         
XNASS REDExtract-N-Amp™ Seed PCR Kit 10 extractions,
10 amplifications
1 seed <15 min from extraction to amplification
XNAS REDExtract-N-Amp™ Seed PCR Kit 100 extractions,
100 amplifications
1 seed <15 min from extraction to amplification