Purified Plasmid DNA is Especially Suited for Automated Sequencing

Capillary DNA sequencing of plasmid DNA purified with the GenElute Five-Minute Plasmid Miniprep Kit. pCMV-SPORT-ßgal (7.9 kb) was prepared from 400 µl overnight culture of E. coli host strain DH5a with the GenElute Five-Minute Plasmid Miniprep Kit. A 5 µl sample of the preparation was submitted, with adjustment in DNA concentration, to SeqWright DNA Technology Service (Houston, TX) for sequencing with a T7 sequencing primer and ABI BigDye™ terminator v3.1 on an ABI Prism® 3730 DNA Analyzer. The Phred > 20 score was 905 bases.

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