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DNA & RNA Purification

HP Plasmid Kits Home Page


The GenElute™ HP Plasmid Midiprep and Maxiprep Kits offer purification of high quality plasmid DNA in less than 30 minutes (Figure 1). GenElute™ HP Plasmid Kits feature a filter syringe for lysate clearing, and binding columns that can be used with a vacuum or spin purification format.

An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis procedure. The lysate is cleared with a filter syringe and DNA is bound to the silica-based membrane. The remaining contaminates are removed by a wash step. Finally, the bound DNA is eluted in buffer or water. High quality DNA is suitable for the most demanding applications including transfection (Figure 3), restriction digestion, ligation, automated sequencing.

 

Features and Benefits

  • From harvested bacterial culture to pure plasmid DNA in 30 minutes or less
  • Up to 350 µg (Midi) and 1.2 mg (Maxi) yield of high-copy plasmid DNA (Figure 2)
  • Offers the flexibility of a vacuum or spin format

Purified Plasmid DNA
Actual Time: Under 30 minutes

1) Resuspend and
Lyse cells

3-5 minutes

2) Neutralize in
filter syringe

5 minutes


3) Prepare Column
Add solution and vacuum

4) Clear lysate
and bind DNA

Filter lysate directly
onto column and
apply vacuum -
5 minutes


5) Wash
Add wash solution
and allow column
to dry on vacuum -
10 minutes


6) Elute
Centrifugation -
5 minutes


 
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