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DNA & RNA Purification

Technical Articles

GenElute™ HP Plasmid Kits
GenElute™ Bacterial Genomic DNA Miniprep Kit
GenElute™ Blood Genomic DNA Miniprep Kit
GenElute™ Mammalian Genomic DNA Miniprep Kit
GenElute™ Plant Genomic DNA Miniprep Kit
GenElute™ Gel Extract Kit
GenElute™ PCR Clean-Up Kit
GenElute™ Mammalian Total RNA Kit
GenElute™ mRNA Kits
Extract-N-Amp™ Seed
Extract-N-Amp™ Blood
Extract-N-Amp™ Plant
Extract-N-Amp™ Tissue

GenElute™ HP Plasmid Kits

GenElute™ HP Endotoxin-Free Maxiprep Kit: High Quality Plasmid DNA for Transfecting Sensitive Eukaryotic Cell Lines

Removal of Contaminating DNA from Commercial Nucleic Acid Extraction Kit Reagents

Chemically Induced DNA Hypomethylation in Breast Carcinoma Cells

The Role of Inducible Repressor Proteins in the Adrenergic Induction of Arylalkylamine-N-Acetyltransferase and Mitogen-Activated Protein Kinase Phosphatase-1 in Rat Pinealocytes

Daidzein-Sulfate Metabolites Affect Transcriptional and Antiproliferative Activities of Estrogen
Receptor-β in Cultured Human Cancer Cells

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GenElute™ Bacterial Genomic DNA Miniprep Kit

The Legionella Autoinducer Synthase LqsA Produces an α-Hydroxyketone Signaling Molecule

Multiplex PCR for Simultaneous Detection of Macrolide and Tetracycline Resistance Determinants in Streptococci

A Non-redundant Microarray of Genes for Two Related Bacteria

Application of Molecular Fingerprinting for Qualitative Assessment of Small-Intestinal Bacterial Diversity in Dogs

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GenElute™ Blood Genomic DNA Miniprep Kit

Randomly Amplified Polymorphic DNA as a Tool for Genotoxicity: An Assessment

Identification and Molecular Modelling of a Novel Familial Mutation in the SRY Gene Implicated in the Pure Gonadal Dysgenesis

Clinical Usefulness of K-RAS Mutation Detection in Colorectal Cancer and in Surgical Margins of the Colon

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GenElute™ Mammalian Genomic DNA Miniprep Kit

Mytilus galloprovincialis-type Foot-protein-1 Alleles Occur at Low Frequency among Mussels in the Dutch Wadden Sea

Randomly Amplified Polymorphic DNA as a Tool for Genotoxicity: an Assessment

Development of a Nested Qualitative Real-Time PCR Assay To Detect Aspergillus Species DNA in Clinical Specimens

Transient Hypoxia Stimulates Mitochondrial Biogenesis in Brain Subcortex by a Neuronal Nitric Oxide Synthase-Dependent Mechanism

The Endosymbiont Wolbachia pipientis Induces the Expression of Host Antioxidant Proteins in an Aedes albopictus Cell Line

Mouse Arylamine N-acetyltransferase 2 (Nat2) Expression during Embryogenesis: a Potential Marker for the Developing Neuroendocrine System

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GenElute™ Plant Genomic DNA Miniprep Kit

Genetic Relationships and Genotype Tracing in Date Palms (Phoenix dactylifera L.) in Oman, Based on Microsatellite Markers

Segregation of DNA Markers of Potato (Solanum tuberosum ssp. tuberosum L.) Resistance against Ro1 Pathotype Globodera rostochiensis in Selected F1 Progeny

The Aspergillus parasiticus estA-Encoded Esterase Converts Versiconal Hemiacetal Acetate to Versiconal and Versiconol Acetate to Versiconol in Aflatoxin Biosynthesis

PCR Markers of Apple Resistance to Scab (Venturia inaequalis CKE.) Controlled by Vf Gene in Czech Apple Breeding

The Genetics of Bambara Groundnut and the Construction of a Genetic Linkage Map

Long Range PCR: Promising Tool for Allelic Mining in Wild Potato Species

A Standardized Protocol for Genomic DNA Isolation from Terminalia arjuna for Genetic Diversity Analysis

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GenElute™ Gel Extract Kit

Maternal Genistein Alters Coat Color and Protects Avy Mouse Offspring from Obesity by Modifying the Fetal Epigenome

High Throughput Detection of M6P/IGF2R Intronic Hypermethylation and LOH in Ovarian Cancer

Phylogenetic Footprint Analysis of IGF2 in Extant Mammals

Two Chemosensory Operons of Rhodobacter sphaeroides are Regulated Independently by Sigma 28 and Sigma 54

p53 Dysfunction in B-cell Chronic Lymphocytic Leukemia: Inactivation of ATM as an Alternative to TP53 Mutation

Isolation of RNA from Polysaccharide-Rich Seeds

Marsupials and Eutherians Reunited: Genetic Evidence for the Theria Hypothesis of Mammalian Evolution

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GenElute™ PCR Clean-Up Kit

Phylogenetic Footprint Analysis of IGF2 in Extant Mammals

Evidence of Chemolithoautotrophy in the Bacterial Community Associated with Alvinella pompejana, a Hydrothermal Vent Polychaete

Porcine Preadipocyte Proliferation and Differentiation: A Role for Leptin?

Aggregation and Adsorption at the Air-Water Interface of Bacteriophage ΦX174 Single-Stranded DNA

Germline and Mosaic Mutations of FLN1 in Men with Periventricular Heterotopia

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GenElute™ Mammalian Total RNA Kit

Protocol for Isolation of Total RNA from Cells

Comparison of Six RNA Extraction Methods for the Detection of Classical Swine Fever Virus by Real-time and Conventional Reverse Transcription-PCR

Dual Effects of Escherichia coli α-Hemolysin on Rat Renal Proximal Tubule Cells

Serum- and Glucocorticoid-Regulated Kinase Isoform-1 and Epithelial Sodium Channel Subunits in Human Ocular Ciliary Epithelium

Expression Profiles of Drug-Metabolizing Enzyme CYP3A and Drug Efflux Transporter Multidrug Resistance 1 Subfamily mRNAs in Rat Small Intestine

Induction of Adipocyte-Like Phenotype in Human Mesenchymal Stem Cells by Hypoxia

C-Reactive Protein Attenuates Endothelial Progenitor Cell Survival, Differentiation, and Function

Biological Identifications Through DNA Barcodes: The Case of the Crustacea

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GenElute™ mRNA Kits

A Novel Receptor on Allograft (H-2d)-Induced Macrophage (H-2b) toward an Allogeneic Major Histocompatibility Complex Class I Molecule, H-2Dd, in Mice

Human Hair Follicles Contain Two Forms of ATP-sensitive Potassium Channels, Only One of which is Sensitive to Minoxidil

Celecoxib Decreases Fatty Acid Synthase Expression via Down-Regulation of c-Jun N-Terminal Kinase-1

Characterization of CCL20 Secretion by Human Epithelial VaginalCells: iInvolvement in Langerhans Cell Precursor Attraction

UbcH7 Interacts with the Glucocorticoid Receptor and Mediates Receptor Autoregulation

RNA Isolation From High-Phenolic Freeze-Dried Tea (Camellia sinensis) Leaves

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Extract-N-Amp™ Seed

Phylogenetic Selationships within Senna (Leguminosae, Cassiinae) Based on Three Chloroplast DNA Regions: Patterns in the Evolution of Floral Symmetry and Extrafloral Nectaries

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Extract-N-Amp™ Blood

T Cells from Patients with Polycythemia Vera Elaborate Growth Factors which Contribute to Endogenous Erythroid and Megakaryocyte Colony Formation

An Inexpensive, Simple Protocol for DNA Isolation from Blood for High-Throughput Genotyping by Polymerase Chain Reaction or Restriction Endonuclease Digestion

Cost-Efficient Sequence-Specific Priming-Polymerase Chain Reaction Screening for Blood Donors with Rare Phenotypes

Effects of Chromatin-Modifying Agents on CD34+ Cells from Patients with Idiopathic Myelofibrosis

Hyperphagia-mediated Obesity in Transgenic Mice Misexpressing the RNA-editing Enzyme ADAR2

Hyperleptinemia Precipitates Diet-Induced Obesity in Transgenic Mice Overexpressing Leptin

Whole Genome Amplification on DNA from Filter Paper Blood Spot Samples: An Evaluation of Selected Systems (Linking to and registering required before viewing this document.)

Whole Genome Amplification and Genetic Analysis after Extraction of Proteins from Dried Blood Spots

Extract-N-Amp™: A Novel Approach for Rapidly Extracting and Amplifying Genomic DNA from Blood and Tissue (158 Kb PDF)

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Extract-N-Amp™ Plant

Optimization of human papillomavirus type 16 (HPV-16) L1 expression in plants: comparison of the suitability of different HPV-16 L1 gene variants and different cell-compartment localization

Telomere-mediated Chromosomal Truncation in Maize

Enhancement of Folates in Plants through Metabolic Engineering

A Protocol for the Rapid Isolation of Full Geminivirus Genomes from Dried Plant Tissue

Efficient Low-cost DNA Extraction and Multiplex Fluorescent PCR Method for Marker-assisted Selection in Breeding

A Rapid-PCR Based Method for the Detection of Magnaporthe oryzae from Infected Perennial Ryegrass

Optimization of Human Papillomavirus Type 16 (HPV-16) L1 Expression in Plants: Comparison of the Suitability of Different HPV-16 L1 Gene Variants and Different Cell-compartment Localization

Detection of Xanthomonas fragariae and Presumptive Detection of Xanthomonas arboricola pv. fragariae, from Strawberry Leaves, by Real-time PCR

Cultivated Helianthus annuus (Asteraceae) Volunteers as a Genetic "Bridge" to Weedy Sunflower Populations in North America

KEEP ON GOING, a RING E3 Ligase Essential for Arabidopsis Growth and Development, Is Involved in Abscisic Acid Signaling

Asian Soybean Rust Caused by Phakopsora pachyrhizi on Soybean and Kudzu in Florida

A PCR Protocol for Rapid Detection of Cercospora beticola in Infected Sugarbeet Tissue

Safflower: A New Host of Cercospora beticola

Identification of Fungi Associated with Grapevine Decline in Castilla y León (Spain)

Improved Detection Methods for Fruit Tree Phytoplasmas

Paratransgenesis to Control Pierce’s Disease: Biology of Endophytic Bacteria in Grape Plants and Bioassay of Reagents to Disrupt Pierces’s Disease

Ecological and Evolutionary Implication of Bt Cotton. Measurement of a Single Gene Difference in Two Cotton Plants by PCR

A High-throughput System for the Rapid Extraction of Plant Genomic DNA for Genome Mapping and Marker-assisted Breeding Studies (1.53 Mb PDF)

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Extract-N-Amp™ Tissue

Reduced Contractile Response to α1-adrenergic Stimulation in Atria from Mice with Chronic Cardiac Calmodulin Cinase II Inhibition

Increased Plasminogen Activator Inhibitor Type-1 (PAI-1) in the Heart as a Function of Age

Allelic Dropout from a High-quality DNA Source

Chromosomal Localization, Hematologic Characterization, and Iron Metabolism of the Hereditary Erythroblastic Anemia (HEA) Mutant Mouse

Depletion of Ascorbic Acid Restricts Angiogenesis and Retards Tumor Growth in a Mouse Model

Role of Toll-like Receptor Signalling in Aβ Uptake and Clearance

Rapid Genotyping of Mouse Tissue Using Sigma®'s Extract-N-Amp Tissue PCR Kit

Lethal Infection of K18-hACE2 Mice Infected with Severe Acute Respiratory Syndrome Coronavirus

Loss of Hepatocyte Growth Factor/c-Met Signaling Pathway Accelerates Early Stages of N-nitrosodiethylamine–Induced Hepatocarcinogenesis

Extract-N-Amp™ Tissue PCR Kit: Rapid Genomic DNA Extraction from Tissue Coupled with PCR

N-ethyl-N-nitrosourea (ENU)-Induced Meningiomatosis and Meningioma in p16INK4aF/p19ARF Tumor Suppressor Gene-Deficient Mice

Detection of the Bacterium, Xylella fastidiosa, in Saliva of Glassy-winged Sharpshooter, Homalodisca vitripennis

Extract-N-Amp™ PCR Kits: A Novel and High-throughput Approach for Rapidly Extracting and Amplifying Genomic DNA from Animal Tissues (1.4 Mb PDF)

Rapid DNA Extraction, Amplification, and Detection System Effective for Mouse Genotyping (269 Kb PDF)

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