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DNA & RNA Purification

Plant Genomic DNA


Description: With the GenElute Plant Genomic DNA Miniprep Kit, high quality genomic DNA can be purified from a variety of plant species (Table 1, Figure 1, and Figure 2). The kit contains all the reagents, columns and tubes necessary to isolate genomic DNA from up to 100 mg of fresh or 20 mg of freeze-dried plant tissue.

Quick look: G2N10, G2N70, G2N350

Procedure: Plant tissue is disrupted by grinding in liquid nitrogen, and DNA is released with detergent and chaotrope. Proteins, polysaccharides, and cell debris are eliminated with a 10 minute precipitation procedure followed by centrifugation through a filtration column, included in the kit. The genomic DNA is purified further by a silica bind-wash-elute procedure in microcentrifuge spin columns. Purified DNA is ready for downstream applications such as PCR (Figure 2), restriction endonuclease digestions, cloning and southern blots.

Features and Benefits:

  • Less than 40 minutes from tissue to purified genomic DNA, including disruption in liquid nitrogen
  • Typical DNA yields of up to 20 µg per prep
  • No RNase treatment required
  • 40% more purifications per kit than the leading supplier

Storage: Room Temperature
R: 20/21/22-32-36/38 S: 26-36

Application: Purified DNA (Figure 2) is ready for downstream applications such as PCR (Figure 3), restriction endonuclease digestions, cloning and southern blots.


GenElute Mammalian Genomic Flow Chart

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Sample Data

Table 1. Typical yields of genomic DNA isolated from various plant species per 100 mg of starting leaf tissue

Material Typical Yield
Corn 7.5 µg
Dianthus tissue culture 3.3 µg
Pepper 3.1 µg
Rice 5.9 µg
Soybean 5.7 µg
Tobacco 5.2 µg
Tomato 6.2 µg
Tomato (20 mg freeze dried leaf tissue) 5.7 µg
Wheat 11.5 µg

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Genomic DNA from various plant species isolated with GenElute Plant Genomic DNA Miniprep Kit.

 

PCR amplification of a 500 bp product isolated from genomic DNA.

Genomic DNA from various plant species isolated with GenElute Plant Genomic DNA Miniprep Kit

Figure 1. Purified genomic DNA (0.4 µg/lane) was analyzed on a 0.8% agarose gel. Markers are Lambda EcoR I (Product No. D9281) Hind III digest.

 

PCR amplification of a 500 bp product isolated from genomic DNA

Figure 2. Genomic DNA from soybean leaves was purified using the GenElute™ Plant Genomic DNA Miniprep Kit. A 5 µl aliquot of eluate was used as template in a 20 µl total PCR reaction for 30 cycles. A 5 µl aliquote of each PCR reaction was resolved on a 2% precast agarose gel (Product No. P5722). The PCR marker (M) used (Product No. P9577) ranged from 50 bp to 2 kb.

 

Genomic DNA Isolation from Rice (GenElute Plant Genomic DNA Kit)

 

Genomic DNA Isolation from Cotton (GenElute Plant Genomic DNA Kit)

Lanes (1) 1Kb DNA ladder; (2) Rice Genomic DNA; (3) λ Hind III digest

 

Lanes (1) 1Kb marker ladder; (2) Cotton Genomic DNA; (3) λ Hind III digest

 

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Supplier Comparison of Preparation Time.
Supplier Comparison of Preparation Time
Figure 3. Comparison of preparation time. The prep time required using the GenElute Plant Genomic DNA Miniprep Kit compared to that of three kits from other suppliers.
The RNase treatment is not always required. The GenElute Plant Genomic DNA Miniprep Kit has been tested for use with corn, dianthus tissue culture, pepper, rice, soybean, tobacco, tomato and wheat. Other sources include Aspergillus parasiticus mold, potato tissue disks, snail and Arbuscular mycorrhizal fungal spores.

Order Information
Product Code Name Purifications per kit Technical Manual .pdf Short
Protocol.pdf
G2N10 GenElute Plant Genomic DNA Miniprep 10 X X
G2N70 GenElute Plant Genomic DNA Miniprep 70 X X
G2N350 GenElute Plant Genomic DNA Miniprep 350 X X

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