Attention:

Certain features of Sigma-Aldrich.com will be down for maintenance the evening of Friday August 18th starting at 8:00 pm CDT until Saturday August 19th at 12:01 pm CDT.   Please note that you still have telephone and email access to our local offices. We apologize for any inconvenience.

DNA & RNA Purification

SigmaSpin™ Post-Reaction Clean-Up Plates and Columns



SigmaSpin Post-Reaction Clean-Up Columns

SigmaSpin Sequencing Reaction Clean-Up Columns are ideal for lower throughput applications, such as clean up of probe labeling reactions or small numbers of sequencing reactions.

These columns can accept sample volumes up to 100 µl. Each column comes with a collection tube to collect the DNA during centrifugation.
 
SigmaSpin 96-Well Sequencing Reaction Clean-Up Plates
SigmaSpin 96-Well Sequencing Reaction Clean-Up plates provide a fast, simple, and highly efficient method for removing unincorporated dyes, excess salts, and other interfering reaction components. Each plate is packed with a size-exclusion resin, prehydrated with molecular biology grade water. Unique plate design incorporates drip directors to eliminate contamination between samples. SigmaSpin has been tested in high-throughput genome centers and core facilities with ABI Prism 3700, 3100, 377 and Amersham Biosciences MegaBACE automated DNA sequencers. SigmaSpin is compatible with all standard terminator chemistries including BigDye, DYEnamic ET and Thermo Sequenase II.

SigmaSpin Sequencing Reaction Clean-Up
 
SigmaSpin Plates and Columns
SigmaSpin 96-Well Clean-Up Plate

Order Information

Product Code Product Name Size
S5059 SigmaSpin Post-Reaction Clean-Up Columns 70 each
S4309 SigmaSpin 96-Well Sequencing Reaction Clean-Up Plates 2 each

back to top




Beneficial Features
Versatile

Compatible with all standard terminator chemistries and all commercially available DNA sequencers

Fast

Quick and easy protocol requires 6 minutes total centrifugation

High Performance

Pre-qualified size-exclusion resin guarantees optimum performance

Recovery

Load and recover sample volumes down to 5 µl

Precision

Unique drip directors prevent cross-contamination between samples

Return to Top

Sample Data

SigmaSpin Removes Interfering Reaction Components and Eliminates Dye Blobs

Figure 1. Sequencing reaction was purified using a SigmaSpin 96-well plate. pFLAG™ MAC plasmid (E 5644) was sequenced using BigDye™ Terminator v 3.0 chemistry (20 µl total reaction volume; BigDye premix diluted 1:1 with SeqSaver™ (S3938)). Data was generated on an ABI Prism. 3700 DNA Analyzer with POP-6™ polymer and 10X CE Buffer (B4930). Phred 20 > 600.

Click Image to View Sequence in its Entirety

Return to Top