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DNA & RNA Purification

RNA Purification

Purify with Sigma

Our selection of GenElute™ RNA purification kits offers simple solutions for RNA isolation. The selection of RNA purification kits:

  • Utilize advanced silica bind and elute technology
  • Are formulated without phenol or chloroform
  • Include kits designed specifically for FFPE, plasma/serum, single cell, plant, water and other sample types are available.
  • Include kits for RNA/DNA co-purification

The GenElute™ selection also includes stand-alone reagents for RNA isolation and RNA/DNA co-purification.
 

Product No. Product Description Starting Amount Time Required Expected Yield
Total RNA
RTN10 (10 preps)

RTN70 (70 preps)

RTN350 (350 preps)
GenElute™ Mammalian Total RNA Miniprep Kit Up to 107 mammalian cells or 40 mg of tissue 30 min Up to 150 µg of total RNA
RNB100-50RXN

RNB100-100RXN
GenElute™ Universal Total RNA Purification Kit Up to 106 mammalian cells or 25 mg of tissue 20 min Up to 50 µg of total RNA
RNB200-8RXN GenElute™ Total RNA Purification Maxi Kit Up to 5x107 mammalian cells or 1 g of tissue 40 min Up to 1.5 mg
RNB300-50RXN GenElute™ Single Cell RNA Purification Kit Single cell to 2x10cells 20 min Varies depending on starting amount
RTN9602 (96-well x 2)

RTN9604 (96-well x 4)
GenElute™ 96 Well Total RNA Purification Kit Up to 107 cultured cells or 30 mg of tissue 70 min 50-200 ng/µL
mRNA
MRN10 (10 preps)

MRN70 (70 preps)
GenElute™ mRNA Miniprep Kit 5-500 µg total RNA 40 min 1-5% of starting total RNA
DMN10 (10 preps)

DMN70 (70 preps)
GenElute™ Direct mRNA Miniprep Kit Up to 107 mammalian cells or 50 mg of tissue 60 min Up to 5 µg
microRNA
SNC10 (10 preps)

SNC50 (50 preps)
mirPremier® microRNA Isolation Kit Up to 107 cultured cells or 40 mg of tissue 30 min Up to 20 µg
R4533-50ML

R4533-100ML

R4533-200ML
RNAzol® RT 1 mL sufficient for 107 cells or 100 mg of tissue < 1 hour 0.1 µg/ µL of microRNA  
RNA/DNA Co-Purification
RDP300-50RXN GenElute™ RNA/DNA/Protein Plus Purification Kit Up to 5 x 106 cells
or 25 mg of tissue
30 min For 106 HeLa cells, avg yield of 15 μg RNA, 8 μg DNA, and 150 μg protein
T9424-25ML

T9424-100ML

T9424-200ML
TRI Reagent® Up to 107 cells or 100 mg of tissue 90 min 5-15 µg/106 cells; 1-10 µg/mg tissue
E4788 (50 preps) Plant RNA/DNA Purification Kit Up to 5 x 106 cells  or 100 mg of tissue 30 min Up to 50 µg for RNA; 15 µg for gDNA
RDP100-50RXN GenElute™ Water RNA/DNA Purification Kit – 0.45µm 10 – 100 mL 45 min 100 µL
RDP200-50RXN GenElute™ FFPE RNA/DNA Purification Plus Kit Up to 10 mg block or 4 sections <20 µM thick 1 – 4 hrs; varies depending on incubation time and formalin crosslink-reversal Up to 35 µg for RNA; 10 µg for DNA
Special Samples
STRN10 (10 preps)

STRN50 (50 preps)

STRN250 (250 preps)
Spectrum™ Plant Total RNA Kit Up to 100 mg of ground plant tissue 30 min 20-60 µg
E4913 (50 preps) Plant/Fungi RNA Purification Kit Up to 50 mg plant tissue; 1 x 106 cells; 50 mg of fungi 30 min Up to 60 µg; varies depending on starting material
RNB400-50RXN GenElute™ FFPE RNA Purification Kit Up to 25 mg block or 5 sections of 20 µm thick paraffin slices 1 – 4 hrs; varies depending on incubation time and formalin crosslink-reversal Up to 50 µg
RNB500-50RXN GenElute™ Plasma/Serum RNA Purification Kit 50 – 200 µL 15 – 20 min Varies depending on specimen
RNB600-20RXN GenElute™ Plasma/Serum RNA Purification Midi Kit 250 µL – 1.5 mL 35 – 40 min Varies depending on specimen
RNB700-10RXN GenElute™ Plasma/Serum RNA Purification Maxi Kit 2 mL – 5 mL 35 – 40 min Varies depending on specimen