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DNA & RNA Purification

Total RNA Bacteria Purification Kit


Sigma’s GenElute Bacterial Total RNA Purification Kit combines silica-membrane technology with a convenient spin column format for a rapid bind, wash and elute method to prepare high quality total RNA from both Gram-negative and Gram-positive bacteria.

Cells are lysed and homogenized in a guanidine thiocyanate-containing buffer to ensure thorough denaturing of macromolecules and inactivation of RNases. Addition of ethanol causes RNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. After washing to remove contaminants, total RNA is then eluted. The purified RNA is ready for reverse transcription, PCR†, labeling, microarray analysis, and other common applications.


Features and Benefits
  • Pure - High quality RNA in less than one hour

  • Flexible - Suitable for both Gram-positive and Gram-negative bacteria

  • Robust - Yields of 30 - 55 µg

  • Efficient - Purified RNA is ready for RT-PCR, Northern Blots, and Microarray analysis.

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GenElute Bacterial Total RNA Purification Kits
Product Code Product Name Purifications
per kit
BTR1-1KT GenElute Bacterial Total RNA Purification Kit 10
BTR7-1KT GenElute Bacterial Total RNA Purification Kit 70


Superior Yields of RNA

    1       2       3         4         5         6       7      

Figure 1.R7644, RNA marker. Lanes 5,6,7 show increasing cell mass respectively using Supplier “Q” kit. Sigma’s kit shows increasing yields with increasing cell mass whereas the competitor kit achieves lower yields even with higher cell mass inputs.

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Source   Type of Media   Amount of Source Material   OD600   Typical RNA Yield**  
Esherichia coli
ATCC# 11775
  LB Broth
(Cat. Code L3522 )
  1.0 ml   0.63   45 - 55 µg  
 
Bacillus subtilis
ATCC# 6051
  LB Broth
(Cat. Code L3522 )
  1.33 ml   0.75   30 - 35 µg  
 

Figure 2. Typical RNA yield with the GenElute Bacterial Total RNA Purification Kit.
*All readings were obtained using a Molecular Devices SpectraMax Plus Spectrophotometer.
**Based on performing 50 µl elutions.



High Quality RNA

Figure 3. Pure, intact E. coli RNA of high integrity is demonstrated by the Agilent 2100 Bioanalyzer electropherogram.

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