PCR/Amplification

Human Genomic DNA

 

Human Random Control DNA Panels for use as reference standards in routine assays.


Sigma-Aldrich and ECACC have teamed together to provide researchers with control populations of human genomic DNA for gene regulation and quantitative PCR research.
Sigma and ECACC Team to Provide Human Genomic Control DNA
View Press Release
(60 kb PDF)

The range of Human Random Control (HRC) DNA samples represents a control population of 480 UK Caucasian blood donors. The HRC DNA is extracted from lymphoblastoid cell lines derived by Epstein Barr Virus (EBV) that can be continuously propagated in culture. This ensures an infinite supply of the unvarying DNA panels. The composition of each panel is completely defined and standardized so that each lot will be identical. Therefore, the HRC DNA Panels can be used as Reference Standards as routine quality control in the laboratory.

 

  • Consistent Control Samples
    The DNA is extracted from immortalized cell lines held as cryopreserved banks, so there is no batch-to-batch variation.
  • Convenient and Ready to Use
    Ideal for most standard genetic research applications and avoids errors in preparing controls.
  • Cost Effective
    The 2 µg format provides enough purified HRC DNA for thousands of PCR assays.
  • Standardized
    The 2 µg of each DNA sample is provided at a standard concentration of 100 ng/µl
  • Convenient format
    The HRC DNA is available as a series of five panels (HRC 1 through 5) each containing samples from 96 separate individuals in a convenient 8 x 12 well format. DNA is provided as a solution in 10 mM Tris buffer (pH 8.0) with 1 mM EDTA

 


Increased effectiveness and reliability of HRC DNA Panels



Comparison of 96 ECACC HRC DNAs and 384 DNAs from another source using the same 5' nuclease assay.

a) A reduced scatter and tighter clustering using ECACC HRC DNA
b) Using DNA from another source has resulted in increased scatter and poor grouping, decreasing the effectiveness of the assay and the reliability of the data (data obtained from MRC geneservice, Cambridge, a division of the MRC HGMP UK Resource Centre).


Use of European Collection of Cell Cultures (ECACC) DNA Control Panels (HRC–1) in the Quality Control of High Throughput SNP Studies at MRC geneservice.


Ordering Information

Product Name Product #  Package Size Concentration
Human Random Control Panel 1 HRC1 1 ea 100 ng/µl
Human Random Control Panel 2 HRC2 1 ea 100 ng/µl
Human Random Control Panel 3 HRC3 1 ea 100 ng/µl
Human Random Control Panel 4 HRC4 1 ea 100 ng/µl
Human Random Control Panel 5 HRC5 1 ea 100 ng/µl
Human Genetic Disease - Breast Cancer HGDBC1 96-well plate 8 ng/µl

For ordering purposes, the 96-well plate format is listed as a 1 EACH (EA) package size.


To order, call 800-325-3010.