PCR/Amplification

LuminoCt™ qPCR Reagents

Make your qPCR data shine

Introducing the next generation of qPCR reagents from Sigma® Life Science. Based on a highly optimized ReadyMix™ chemistry, LuminoCt delivers unsurpassed assay speeds without sacrificing accuracy, precision, or sensitivity. And best of all, LuminoCt works seamlessly with most qPCR instruments, so optimization of reaction conditions is kept to an absolute minimum. Often, users can simply choose their kit, build their assay, and begin. Generating the best possible qPCR data has never been faster or easier.

Switch to LuminoCt and discover:

  • Speed – assay results in as little as 25 minutes
  • Quality – unsurpassed accuracy, precision, and sensitivity
  • Simplicity – virtually eliminates the need to optimize assay conditions

Time Comparison
Amplification Plot
Standard Curve
How to Switch
Ordering Information



Time Comparison



Wide Dynamic Range and High Sensitivity



Amplification Plot
Log-fold dilutions of human fibroblast genomic DNA (108 to 103 fg) were assayed in triplicate using LuminoCt qPCR ReadyMix and a dual-labeled fluorescent probe and primers targeting the 18S ribosomal RNA gene. Assays were performed on an ABI 7500 Fast qPCR instrument according to the manufacturers recommended protocol.



Accurate and Precise Results



Standard Curve
The standard curve was generated on an ABI 7500 Fast instrument by plotting the threshold cycle (Ct; see Amplification Plot above) versus the log of the initial quantity of DNA for each reaction.



How to Switch

Switch in 3 simple steps:

  1. Select kit(s) to match desired detection technology
  2. Create rapid protocol(s) for qPCR assays. Sigma recommends*:
    Initial denaturation:95°C for 20 sec
    40 PCR cycles:95°C for 3 sec
    60°C for 15–30 sec
  3. Ensure primers and probes are compatible with rapid assay protocols
     i. Design Primer sets with amplicons of <200 bp; or
    ii. Use Commercially available primer and probe sets (e.g., TaqMan® Assays)

*Reagents are also compatible with the protocols of the following instruments: ABI 7900HT Fast, ABI 7500 Fast, ABI StepOne, Roche LC 480, Bio-Rad CFX96 and CFX 384, and the Eppendorf Mastercycler ep Realplex.



Ordering Information

More information on LuminoCt products.


Product No. Description Quantity 2X ReadyMix Volume
L6544 LuminoCt SYBR® Green qPCR ReadyMix 100RXN 2.5 ml
500RXN 12.5 ml
2000RXN 50 ml
L6669 LuminoCt qPCR ReadyMix 100RXN 2.5 ml
500RXN 12.5 ml
2000RXN 50 ml

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