PCR/Amplification

Quantitative RT-PCR

Sigma’s RT-qPCR products combine the effective of Reverse Transcriptase with hot-start taq-directed antibody in convenient ReadyMixes for probe-based or SYBR® Green based applications. Regardless of difficult secondary structures or detection chemistry, our ReadyMixes are specially formulated to help you achieve superior results in fewer steps. Our products are easy to explore and understand; explore our JumpStart options for kits optimizable to your experimental needs or choose KiCqStart® and find a ReadyMix™ optimized for your instrument! If you’re looking for microRNA Reverse Transcription, take a look at our MystiCq® product line.

Not sure what to look for? Check out our PCR Selection Guide.
 

RT-PCR  |  qPCR  |  Routine PCR  |  Custom Probes

RNA:

  • KiCqStart – Formats for all major qPCR instruments
  • JumpStart™ – Optimizable for all major qPCR instruments

MicroRNA:

 RNA-Based RT-qPCR

KiCqStart – Instrument Optimized

Our KiCqStart ReadyMixes feature a stringent hot-start mechanism for greater specificity . These products are formulated to be highly tolerant of PCR inhibitors in samples. The ReadyMixes are designed for convenience; all required basic components for RT-qPCR are included, simply add primers, probe, and water to complete the assay cocktail. This product is ideal for fast or conventional qPCR.

Learn more about KiCqStart

Check out our Custom Probes

Product No. Product Name Features
KCQS07 KiCqStart One-Step Probe RT-qPCR ReadyMix
• For Bio-Rad, Cepheid, Eppendor, Illumina, Corbett, and Roche Instruments
KCQS08 KiCqStart One-Step Probe RT-qPCR ReadyMix, Low ROX™
• For ABI and Stratagene instruments
KCQS09 KiCqStart One-Step Probe RT-qPCR ReadyMix, ROX
• For ABI instruments

 

JumpStart – Highly Optimized

Our JumpStart RT-qPCR Readymix combines the advantages of Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) and our JumpStart antibody-inactivated Taq polymerase. Robust M-MLV RT has the ability to transcribe through difficult secondary structures, even at elevated temperatures and our JumpStart taq provides greater specificity and sensitivity than standard Taq.

Our products are compatible with both tube/plate based instruments and capillary based instruments.
 

Product No. Product Name Features
QR0100 SYBR Green RT-qPCR • High specificity, low contamination risk, easy reproducibility
QR0200 RT-qPCR ReadyMix for Probe-Based Applications • High specificity, low contamination risk, easy reproducibility

 

 MicroRNA-Based RT-qPCR

MystiCq

MystiCq microRNA reagents provide a complete SYBR Green RT-qPCR workflow for quantifying expression in three steps:

  1. Isolation
  2. cDNA Synthesis
  3. Quantitation by amplification.
  • Over 1400 pre-designed and wet lab tested primer pairs designed to target only mature microRNAs
  • Conversion of all microRNAs, allowing nearly unlimited readouts from a single sample
  • Selection of three different products for isolation of microRNAs

Check out our MystiCq products and Workflow here.