PCR

REDTaq


Our RED enzymes offer speed and simplicity
for every PCR application!

The direct load capability of Sigma's patented line of RED enzymes provides a time-saving reduction in steps between PCR and agarose gel analysis. RED enzymes are a unique blend of our quality DNA polymerases combined with an inert red dye. In addition to reducing transfer steps, this dye provides visual confirmation of proper mixing of the PCR reaction components and ensures uniformity between samples. This Red formulation has been incorporated into all of our PCR product areas — Routine PCR Amplification, Hot Start PCR, Long and Accurate, and Genomic DNA Amplification.
 
For even greater simplicity and time savings, use our RED enzyme ReadyMixes. These mixes are provided as a 2x optimized blend of RED enzyme, 99% pure dNTPs, MgCl2, and PCR buffer. Simply add primers and template to the final reaction volume and begin cycling.

RED enzymes provide:

  • A reduction in steps for high throughput applications
  • Visual confirmation that enzyme has been added and proper mixing has occurred
  • A variety of options to meet all areas of PCR research

 

 

No loading buffers or tracking dyes required

REDTaq DNA Polymerase

Samples may be added directly to an agarose gel after PCR amplification without the addition of a loading buffer or tracking dye. The dye in REDTaq acts as a tracking dye migrating at approximately the same rate as a 125 bp fragment.
 


"Sigma's JumpStart™ REDTaq ReadyMix™ PCR Reaction Mix has helped me generate great data and quickly! It gives excellent specificity and yield when applied to high throughput genetic screening of small amounts of mouse-tail tissue. The fewer number of steps in the PCR procedure result from having the red tracking dye, buffer and dNTPs already in the mix, cutting the process time in half."

Andrew J. Paterson,
University of Alabama at Birmingham
Medicine/Endocrinology, Diabetes and Metabolism




Product Area
Product Code
Product Name
Package Size
Routine Amplification D4309 REDTaq DNA Polymerase with 10X Reaction Buffer containing MgCl2 50 units
250 units
1000 units
2500 units
R2523 REDTaq ReadyMix PCR Reaction Mix 20 reactions
100 reactions
D6063 REDTaq Superpak DNA Polymerase 50 units
250 units
Hot Start D8187 JumpStart REDTaq DNA Polymerase 50 units
250 units
2500 units
P0982 JumpStart REDTaq ReadyMix PCR Reaction Mix 20 reactions
100 reactions
P1107 JumpStart REDTaq ReadyMix for High Throughput PCR 100 reactions
400 reactions
D1313 JumpStart REDAccuTaq LA DNA Polymerase 50 units
250 units
Long and Accurate D4812 REDAccuTaq LA DNA Polymerase 50 units
250 units
2500 units
D1816 RED Klentaq DV ReadyMix 20 reactions
100 reactions
Genomic DNA D8312 REDTaq Genomic DNA Polymerase 50 units
250 units
1000 units
2500 units
D2812 REDTaq Genomic DNA Polymerase without MgCl2 50 units
250 units
1000 units
2500 units