Whole Genome Amplification

Single Cell Kits

The GenomePlex® Single Cell Whole Genome Amplification Kit (WGA4) is designed to amplify the genome of a single cell resulting in a million-fold amplification yielding microgram quantities of genomic DNA. Traditional single cell whole genome amplification methods yield insufficient quantities with significant biased representation. However, the GenomePlex technology provides amplification efficiency with WGA DNA Polymerase. The Single Cell WGA kit includes all of the reagents necessary for cell lysis and subsequent whole genome amplification.

The Single Cell WGA kit has been validated with numerous downstream applications and is suitable for amplification from a variety of sources including: plant, polycarbonate fixed cells, lymphocytes, renal cancer cells, epithelial cells, leukemia cells, and fibroblast amniotic cells. The amplified genome is compatible with many downstream applications including: comparative genomic hybridization (CGH), microsatellite/STR analysis, SNP analysis, QPCR, and other genotyping analysis.

Features & Benefits

  • Abundant yields of DNA generated from single cell genomic DNA in less than four hours
  • Accurate representation of entire genome
  • Identification of genome-wide copy number and chromosomal abnormalities
  • No detectable amplification product for the negative control

Application Data

Source Material
Plant (various types)
lymphocytes
renal cancer cells
epithelial cells
leukemia cells
fibroblast amniotic cells
polycarbonate fixed cells

Downstream Application
Gel electrophoresis & QPCR
CGH microarray
STR analysis
SNP analysis
Other genotyping


Technical Papers
Whole Genome Amplification for Single Cell Biology (123 Kb PDF)
Single Cell Whole Genome Amplification Technique Impacts the Accuracy of SNP Microarray Based Genotyping and Copy Number Analyses (553 Kb PDF)

Single Cell WGA Posters
Chromosome Analysis of Single Cells by High Resolution Comparative Genomic Hybridization after Whole Genome Amplification Using a Random Fragmentation Approach (428 Kb PDF)

Single Cell Aneuploidy Analysis by High Resolution Comparative Genomic Hybridization (CGH) after Whole Genome Amplification reveals Maternal Cell Contamination in the Original Products of Conception Cultures (140 Kb PDF)

Single Cell Whole Genome Amplification: Unleashing a World within a Cell (547 Kb PDF)

Ordering Information

Product Code Product Name Technical Bulletin
WGA4 GenomePlex® Single Cell Whole Genome Amplification (WGA) Kit

GenomePlex® WGA kits are not for use for third party services or diagnostic applications. For information regarding services for whole genome amplification or whole transcriptome amplification of population, clinical samples, or diagnostics applications, contact Rubicon Genomics (www.rubicongenomics.com).

References

  • Barker, D.L. et al. Two methods of whole-genome amplification enable accurate genotyping across a 2320-SNP linage panel. Genomic Research, 14, 901-7 (2004).
  • Gribble, S. et al. Chromosome paints from single copies of chromosomes. Chromosome Research, 12, 143-51 (2004).
  • Treff, N.R. et al. Mol. Hum. Reprod. Advance Access published December 21, 2010.

GenomePlex and OmniPlex are Registered Trademarks of Rubicon Genomics. This product is for research use only. Use of this product for human or animal therapeutic or diagnostic purposes requires licensing from Rubicon Genomics, Inc. Unauthorized use violates proprietary rights of Rubicon Genomics, Inc (www.rubicongenomics.com).

TaqMan is a registered trademark of Roche Molecular Systems, Inc.


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