Proligo® Phosphoramidites and Reagents for Oligonucleotide Synthesis

Through our Proligo® Reagents portfolio, Sigma-Aldrich offers a comprehensive range of products and services for oligonucleotide synthesis.

  • High quality standard and specialty phosphoramidites, solid supports, columns, linkers and liquid formulations
  • Flexible manufacturing, packaging and distribution capabilities
Proligo Catalog - Now Available

Now available, the revised Proligo Reagents Catalog 2014-2016. The most up-to-date catalog featuring all product & technical information associated with the Proligo Reagents range.


 


 

 

 

The Oligonucleotide Synthesis Workflow

 

synthesis cycle

Sigma-Aldrich has a complete product portfolio for solid phase oligonucleotide synthesis ranging from controlled pore glass and phosphoramidites to the whole family of synthesis reagents including custom formulations.

Start of Synthesis: Controlled Pore Glass (bulk or prepack)

Detritylation: Deblock

Activation: Activators

Coupling:

Capping: Cap A and Cap B

Oxidation: Oxidizers

Solvents and Labware
View our complete range of Acetonitrile, Solvents and Labware for every phase of Oligonucleotide Synthesis.

Improve yields and reduce unwanted side reactions with high quality solvents and reagents used in cleavage and deprotection including:

Solvents and Labware
View our complete range of Acetonitrile, Solvents and Labware for every phase of Oligonucleotide Synthesis.

desalting

Sigma-Aldrich can help with the removal of organic salts through a variety of methods including:

Size Exclusion - see more info on General Electric NAP Columns

Reverse Phase Catridge Purification - see more information on Solid Phase Extraction products

Extraction: Butanol

Solvents and Labware
View our complete range of Acetonitrile, Solvents and Labware for every phase of Oligonucleotide Synthesis.

purification

Access a wide variety of products and tools for use in determining product composition and purity profile.

Solvents and Labware
View our complete range of Acetonitrile, Solvents and Labware for every phase of Oligonucleotide Synthesis.

synthesis cycle

Oligonucleotides are manufactured on custom designed high throughput synthesis or commercially available platforms. Sigma-Aldrich formulates and packages ready-to-use reagents for all commercial synthesizers.

Platform:

Solvents and Labware
View our complete range of Acetonitrile, Solvents and Labware for every phase of Oligonucleotide Synthesis.

Make RNA Using Proligo's New TC RNA Chemistry

Watch our video to see how you can make RNA oligonucleotides more efficiently using Proligo TC RNA Chemistry. TC RNA Chemistry provides significant advantages over other RNA amidites:

  • TC monomers couple at significantly higher rates
  • Deprotection occurs in only 2 hours at room temperature

These features provide:

  • Time savings
  • Increased production efficiencies
  • Increased revenues

 

Capabilities and Services

Proligo Quality Assurance - HPLC MS
Quality Assurance
Dedicated process technology group and in-process controls that will adapt to evolving diagnostic and pharmaceutical requirements.

 

Contact Us
For more information about our products and services, request a quote or give us feedback on the type of information you would like to see on this page, please Contact Us.
Packaging Services
State-of-the-Art Production
Complete packaging services from routine to custom and from milligrams to kilograms at our state-of-the-Art Manufacturing facility in Hamburg.