Microcon® Centrifugal Filters

Simply and efficiently concentrate and desalt solutions of any macromolecule, using any centrifuge that can accept 1.5 mL tubes. With the low-binding Ultracel® membrane, Microcon® filters offer:

  • Typical recoveries of >95%, even for dilute solutions
  • Reverse spin to maximize recovery, even in the smallest samples
  • Convenient storage of filtrate or concentrated sample in standard microfuge tube
  • Concentration factors up to 100X

 Microcon® DNA Fast Flow PCR Grade Filter

Working with low concentrations of DNA? Purifying DNA prior to PCR amplification? Use the PCR grade Microcon® ultrafiltration device. The dual cycle Ethylene Oxide (EtO) treated Microcon® DNA Fast Flow PCR grade devices enable centrifugal concentration at a similar rate as the Microcon® DNA Fast Flow devices, but the EtO treatment greatly reduces the risk that no uncontrolled contaminating nucleic acids enter the analysis process.

 

 Microcon® DNA Fast Flow Filter

Optimized for the concentration and recovery of genomic DNA with SDS buffer. The low nonspecific binding characteristics of the membrane and the other device components, coupled with its medical-grade o-ring seal, allows the device to accommodate several wash steps with minimal sample loss.

 

 Features

  • High recovery for small volumes with reverse spin (concentration factor <20x) li="">Low-binding Ultracel® membrane
  • Fast processing

 Sample applications

Applications Guidelines

Use the following table to choose the correct Microcon® device for your application.
 

  Microcon® Device
Application 10K 30K DNA Fast Flow
Peptide and growth factor concentrationPeptide and growth factor concentration  •    
Protein concentration and desalting of columns eluates  •  •  
Protein concentration before electrophoresis or other assays •   
Protein removal prior to HPLC  
Purification of macromolecular components found in tissue culture extracts and cell lysates  
Concentration of biological samples (antigens, antibodies, enzymes)    
Concentration of gDNA with or without SDS buffer  
Concentration and desalting of nucleic acids (single-or double-stranded)
Removal of labeled nucleotides
Removal of labeled amino acids
Removal of primers from amplified DNA  
Removal of linkers prior to cloning  

 

Specifications
 

Maximum initial sample volume 0.5 mL (500 μL)
Typical final concentrate volume 5–50 μL
Maximum relative centrifugal force
Microcon® 10K devices 14,000 × g
Microcon® 30K devices 14,000 × g
Microcon® DNA Fast Flow devices 500 × g
Recovery spin 1,000 × g
Active membrane area 0.32 cm2
Hold-up volume ≤ 10 μL
Dimensions
Diameter 12.3 mm (0.5 in.)
Length (filter device and tube in concentration mode) 45.0 mm (1.8 in.)
Length (filter device and tube in recovery mode) 48.2 mm (1.9 in.)
Materials of Construction
Membrane Ultracel® low binding regenerated cellulose
Device top Polycarbonate
Membrane support base Acetal
Filtrate/concentrate tube Polypropylene
O-ring Medical-grade silicone rubber