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Phosphorylation

Pngase F




PNGase F Proteomics Grade, (P 7367)

This highly purified and very stable endoglycosidase is optimized for the efficient release of N-linked oligosaccharides known to be important in structure-function analysis. Ideal for post-gel electrophoresis or solution based assays. Suitable for both proteomics and glycobiology applications and compatible with MALDI-TOF MS analysis.

This enzyme is a native glycoaminidase cleaving the link between asparagine and N-acetylglucosamines. Treatment with PNGase allows you to study the N-linked carbohydrates in a single protein or mixture. Whether using electrophoresis, chromatography or another method of glycoprotein isolation, you can use this individually or in conjunction with other glycosidases determine the complete carbohydrate content. Useful in the discovery and analysis of glycomes and potential glycotherapeutics.


Features & Benefits

  • Very stable
    Lyophilized to allow appropriate reconstitution for application and ensure optimal activity
  • Highly Purified
    No degradation from contaminating proteases/glycosidases provides unambiquous MALDI-MS spectra
  • High specific activity
    Efficient and complete removal of available N-linked carbohydrates
  • Low Salt
    No interference in MALDI-MS analysis from contaminant buffer agents
  • Convenient pack sizes
    Use only the enzyme needed and eliminate waste

 

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