Protein Electrophoresis

Isoelectric Focusing


ProteoGel™ IPG Strips

Isoelectric focusing (IEF) represents the first dimension of two- dimensional (2D) electrophoresis, and immobilized pH gradient (IPG) strips facilitate this analysis. Each sample protein applied to an IPG strip will migrate to its isoelectric point (pI), the point at which its net charge is zero. ProteoGel IPG strips are available in three lengths to accommodate various gel sizes and six pH ranges to allow optimal separation.

Features & Benefits

  • Narrow and wide range strips with overlap options, in three lengths, allow optimal resolution of most protein samples
  • Control in manufacturing ensures reproducible performance
  • IPG strips reduce preparation time and reduce reagent waste
  • Strips are labeled for polarity to ensure proper orientation

 

Manufactured by Proteome Systems™

Product #  Product Name Size
I2531 7cm, pH 3-10, ProteoGel IPG Strips 12 each
I3031 7cm, pH 3-5, ProteoGel IPG Strips 12 each
I2906 7cm, pH 4-7, ProteoGel IPG Strips 12 each
I3156 7cm, pH 5-8, ProteoGel IPG Strips 12 each
I7406 7cm, pH 6-11, ProteoGel IPG Strips 12 each
I3281 7cm, pH 8-11, ProteoGel IPG Strips 12 each
I3406 11cm, pH 3-10, ProteoGel IPG Strips 12 each
I3656 11cm, pH 3-5, ProteoGel IPG Strips 12 each
I3531 11cm, pH 4-7, ProteoGel IPG Strips 12 each
I3781 11cm, pH 5-8, ProteoGel IPG Strips 12 each
I7531 11cm, pH 6-11, ProteoGel IPG Strips 12 each
I3906 11cm, pH 8-11, ProteoGel IPG Strips 12 each
I4031 18cm, pH 3-10, ProteoGel IPG Strips 12 each
I4281 18cm, pH 3-5, ProteoGel IPG Strips 12 each
I4156 18cm, pH 4-7, ProteoGel IPG Strips 12 each
I4406 18cm, pH 5-8, ProteoGel IPG Strips 12 each
I7656 18cm, pH 6-11, ProteoGel IPG Strips 12 each
I4531 18cm, pH 8-11, ProteoGel IPG Strips 12 each

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ProteoGel™ IPG Equilibration Buffer

ProteoGel IPG Equilibration Buffer has been specially formulated to simplify the transition between first and second dimensional analysis. Following isoelectric focusing, IPG strips must be equilibrated to further denature proteins, maintain solubility, and establish an appropriate pH for SDS- PAGE analysis. Convenient and practical, ProteoGel IPG Equilibration Buffer is compatible with all SDS- PAGE gel systems. Sold as bottles of powder that reconstitute with water to produce 50 ml of solution containing 0.05 M Tris- acetate, 2% SDS, 6 M urea, and 0.0067% bromophenol blue pH 7.0.

Technology developed in partnership with Proteome Systems™

Product #  Product Name Size
I7281 ProteoGel IPG Equilibration Buffer 1 bottle

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ProteoPrep™ Reduction and Alkylation Kit

The ProteoPrep™ Reduction and Alkylation Kit provides tributylphosphine (TBP) and iodoacetamide (IAA) for the reduction and alkylation of protein disulfide bonds. Procedures are provided for the reduction and alkylation of proteins in solution prior to loading the immobilized pH gradient (IPG) strip, during equilibration of the focused IPG strip prior to SDS- PAGE electrophoresis, or in- gel prior to tryptic digestion.

Features & Benefits

  • Reduction and alkylation of proteins - Reduces artifacts due to disulfide bond formation
  • 2D gels have less streaking, increased resolution, fewer artifacts - Better reproducibility when protein samples are reduced and alkylated prior to isoelectric focusing

 

Components
  Tributylphosphine Stock Solution
  Alkylating Reagent lodoacetamide


Product #  Product Name Size
PROT-RA ProteoPrep Reduction & Alkylation Kit 1 kit

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