Western Blotting

Western Buffers Reagents

We carry a variety of buffers and reagents for western blotting. Blocking buffers, transfer buffers, and wash buffers are all available in convenient sized packaging. A variety of reagents are also available, including methanol, dried milk, glycine and several others.


Albumin, Bovine Serum, A9647
Available in 10, 50, and 100 g sizes.


Alkaline Phosphatase Stabilizing Buffer, A4955
Specially made for the stable dilution of alkaline phosphatase-antibody conjugates, this solution contains 1% bovine serum albumin and 15 mM sodium azide in Tris-buffered saline, pH 8.0. Available in 100 mL and 1 L sizes.


CAPS, C2632
Used in Western blotting. Available in 25 g, 100 g, 6 x 100 g, and 250 g sizes.


l-Carrageenan, C3889
This product contains essentially pure lambda carrageenan isolated from two species, Gigartina aciculaire and G. pistillata. Available in 1, 5, 25, and 100 g sizes.


Casein Solution, 5%, from Bovine Milk, C4765
Blocking solution for Western blotting. Available in a 10 mL size.


Gelatin from Cold Water Fish, G7765
This product contains approximately 45% gelatin in water, as well as 0.15% propyl p-hydroxybenzoate and 0.2% methyl p-hydroxybenzoate as preservatives. Available in a 250 mL and 1 L sizes.


Gelatin from Porcine Skin, G8150
Blocking protein for Western blotting. Available in a 100 g and 500 g sizes.


Glutaraldehyde Solution, 25% in Water, G6257
Available in 10 mL, 10 x 10 mL, 100 mL, and 1 L sizes.


Glycine, G8898
Glycine is a component of Towbin's buffer. Available in 500 g and 1 kg sizes.


MES hemisodium salt, M0164
Contents of each pouch dissolved in 1 liter deionized water will yield 0.1 M sodium MES buffer, pH 6.1 at 25°C. Available in 10 pack and 5 x 10 pack sizes.


Methanol, M1775
Available in 1 gallon and 4 x 1 gallon sizes.


Milk, non-fat, dried, bovine, M7409
Reconstitution with 100 mL deionized water yields a 3% w/v solution. Available in 1 bottle and 5 bottle sizes.


MOPS hemisodium salt, M0289
Contents of each foil pouch dissolved in 1 liter of deionized water yield 0.1 M sodium MOPS buffer. Available in 10 pack and 5 x 10 pack sizes.


Peroxidase Stabilizing Buffer, P9209
Specially made for the stable dilution of peroxidase-antibody conjugates, this solution contains 1% bovine serum albumin and an antimicrobial agent in phosphate buffered saline, pH 7.4. Available in 1 L size.


Phosphate Buffered Saline Tablet, pH 7.4, P4417
One tablet dissolved in 200 mL of deionized water yields 0.01 M phosphate buffer, 0.0027 M potassium chloride, and 0.137 M sodium chloride, pH 7.4 at 25°C. Available in 50 tablet and 100 tablet sizes.


Phosphate Buffered Saline 10x Concentrate, P7059
Used in Western blotting. Available in 1 L package size.


Phosphate Buffered Saline, pH 7.4, P3813
Contents of one pouch, when dissolved in one liter of distilled or deionized water, will yield 0.01 M phosphate buffered saline (NaCl 0.138 M; KCl: 0.0027 M); pH 7.4 at 25°C. Available in 1 pack, 10 pack, and 5 x 10 pack sizes.


Phosphate Buffered Saline with Bovine Serum Albumin, pH 7.4, P3688
Contents of one pouch, when dissolved in one liter of distilled or deionized water, will yield 0.01 M phosphate buffered saline (NaCl: 0.138 M; KCl: 0.0027 M); BSA: 1% w/v, pH 7.4 at 25°C. Available in 10 pack size.


Phosphate Buffered Saline with 3% non-fat milk, pH 7.4, P2194
Each pouch dissolved in 100 ml deionized water will yield 0.05 M phosphate buffered saline, pH 7.4 at 25°C, containing 3% w/v non fat milk. Available in 10 pack and 5 x 10 pack sizes.


Phosphate Buffered Saline with TWEEN 20, pH 7.4, P3563
Contents of one pouch, when dissolved in distilled or deionized water, will yield 0.01 M phosphate buffered saline (NaCl: 0.138 M; KCl: 0.0027 M); TWEEN 20: 0.05%, pH 7.4 at 25°C. Available in 10 pack and 5 x 10 pack sizes.


Phosphate Citrate Buffer Tablet, P4809
One tablet dissolved in 100 mL deionized water yields a 0.05 M phosphate citrate buffer, pH 5.0 at 25°C. Available in 50 and 100 tablet sizes.


Phosphate Citrate Buffer with Sodium Perborate Capsule, P4922
The contents of one capsule dissolved in 100 mL of deionized water yields 0.05 M phosphate citrate buffer, containing 0.03% sodium perborate as a substitute for H2O2, pH 5.0 at 25°C. Available in 50 and 100 capsules sizes.


Phosphate Citrate Buffer with Urea Hydrogen Peroxide Tablet, P9305
Phosphate Citrate buffer tablets with Urea Hydrogen Peroxide may be used as a peroxide substrate. Available in 50 tablet and 100 tablet sizes.


Sodium Dodecyl Sulfate (SDS), L3771
Available in 25 g, 100 g, 500 g, and 1 kg sizes.


Tris-Glycine Buffer 10x Concentrate, T4904
Tris-glycine buffer is used to make a Tris-glycine-methanol transfer buffer. The 10x Tris-glycine buffer is diluted to 1x with methanol and water to make a solution containing 25 mM Tris, 192 mM glycine, and 20% methanol. Available in 1 L size.


Tris Buffered Saline Tablets, T5030
One tablet dissolved in 15 ml of deionized water yields 0.05 M Tris and 0.15 M sodium chloride, pH 7.6 at 25°C. Available in 50 and 100 tablet sizes.


Tris Buffered Saline, pH 8.0, T6664
The contents of one pouch, when dissolved in one liter of distilled or deionized water, will yield 0.05 M Tris buffered saline (NaCl: 0.138 M; KCl: 0.0027 M); pH 8.0 at 25°C. Available in 10 pack size.


Tris Buffered Saline with Bovine Serum Albumin, pH 8.0, T6789
Contents of one pouch, when dissolved in one liter of distilled or deionized water, will yield 0.05 M Tris buffered saline (NaCl: 0.138 M; KCl: 0.0027 M); bovine serum albumin: 1% w/v, pH 8.0 at 25°C. Available in 10 pack size.


Tris Buffered Saline with 3% non-fat Milk, pH 8.0, T8793
Contents of one pouch, when dissolved in 100 mL of distilled or deionized water, will yield 0.05 M Tris buffered saline, pH 8.0 at 25°C, containing 3% w/v non-fat milk. Available in 10 pack and 5 x 10 pack sizes.


Trizma Base, T1503
Available in 15 g, 100 g, and 250 g sizes.


Urea, 8M, U6504
Available in 500 g and 1 kg package sizes.


Western Blocker Solution, W0138
Western Blocker solution has been optimized for detection systems using horseradish peroxidase (HRP) conjugates; it is also compatible with alkaline phosphatase based systems. Western Blocker Solution is compatible with both PVDF and nitrocellulose membranes. Available in 400 mL size.


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