ProteoPrep Depletion Products

Product Name Product #  Function Samples Processed Input Sample Size Output Compatibility
ProteoPrep® Immunoaffinity Albumin & IgG Depletion Kit PROT-IA Depletes HSA and IgG from serum or plasma using small recombinant immunoaffinity ligands 10 10µL - 50µL Two-dimensional electrophoresis (2DE)
ProteoPrep® Blue Albumin & IgG Depletion Kit PROT-BA Depletes HSA & IgG with small ligand & full antibody 25 75 µL Two-dimensional electrophoresis (2DE)

ProteoPrep® Immunoaffinity Albumin & IgG Depletion Kit, (PROT-IA)

Depletes HSA and IgG from serum or plasma using small recombinant immunoaffinity ligands

ProteoPrep Immunoaffinity Albumin & IgG Depletion Kit specifically removes albumin and IgG from 25 samples of human serum (25 µl to 100 µl) in preparation for two-dimensional electrophoresis. The ProteoPrep Blue Albumin Depletion Medium is a mixture of two media which both employ small recombinant immunoaffinity ligands. Typical depletions are 95% for albumin and 80% for IgG from 75 µl of human serum. The kit reagents are in urea-based buffers instead of salt-based buffers meaning the albumin-depleted serum samples can be applied in two-dimensional electrophoresis without protein precipitation. Focusing of proteins on immobilized pH gradient (IPG) strips for two-dimensional electrophoresis of proteins and mass spec analysis of in-gel digested spots is negatively impacted (e.g., poor spot resolution) by the presence of salts or high concentrations of buffers. See superior results.


ProteoPrep® Immunoaffinity Albumin & IgG Depletion Kit Technical Bulletin


Features & Benefits

  • Novel antibody affinity media - Displays higher specificity and significantly higher binding capacity than conventional antibody-based resin kits.
  • Reduces albumin and IgG contamination of spots - Improves 2-dimensional electrophoresis and later MALDI identification.
  • More low abundance proteins - Increased loading (4-5 fold) volume of serum onto gel so that lower copy number proteins can be visualized and identified provide improved IEF resolution.
  • Buffers are directly compatible with 2DE - Eliminating the need for protein precipitation procedures prior to electrophoresis.

 


back to top


ProteoPrep® Blue Albumin Depletion Kit, (PROT-BA)

Albumin and IgG are two major protein components of serum representing ~80% of the total serum protein. Removal from Serum allows visualization of co-migrating proteins on a one-dimensional electrophoresis or two dimensional electrophoresis gel and higher sample load (4 to 5-fold) for improved visualization of lower copy number proteins (Figure 1). ProteoPrep Blue Albumin Depletion Kit specifically removes albumin and IgG from 25 samples of human serum (25 mL to 100 mL) in preparation for two-dimensional electrophoresis. The ProteoPrep Blue Albumin Depletion Medium is a mixture of two media: 1) a proprietary blue matrix and 2) Protein G agarose. Typical depletions are 85% for albumin and 70% for IgG from 75 mL of human serum. The medium exhibits low non-specific binding because it does not contain Cibacron® Blue, notorious for high non-specific binding of nonalbumin proteins. The kit reagents are urea-based buffers instead of salt-based buffers meaning the albumin-depleted serum samples can be applied in two-dimensional electrophoresis without protein precipitation. Focusing of proteins on immobilized pH gradient (IPG) strips for two-dimensional electrophoresis of proteins and mass spec analysis of in-gel digested spots is negatively impacted (e.g. poor spot resolution) by the presence of salts (Figure 2) or high concentrations of buffers. Many human serum albumin depletion products available today have salts in the equilibration and wash buffers typically requiring precipitation to remove the salts prior to two-dimensional electrophoresis which is time consuming and risks loss of important proteins. Experiments show that urea can replace salts allowing for albumin binding to a proprietary dye-based resin and inhibiting binding of non-albumin proteins.

ProteoPrep® Blue Albumin Depletion Kit Protocol


Features & Benefits

  • The ProteoPrep Blue media binds and removes 85% of the albumin and 70% of the IgG from 75 µl of serum allowing visualization of lower abundance proteins.
  • Equilibration buffer, a Tris-buffered urea solution, is two-dimensional electrophoresis compatible and does not require sample precipitation.
  • Compatible with sample sizes less than 25 µl without undesirable non-specific binding.

 

back to top
back to Protein Depletion
back to Sample Preparation