Certain features of will be down for maintenance the evening of Friday August 18th starting at 8:00 pm CDT until Saturday August 19th at 12:01 pm CDT.   Please note that you still have telephone and email access to our local offices. We apologize for any inconvenience.

Mammalian Cell Lysis

CelLytic MT

For mammalian tissues, CelLytic MT is an efficient reagent for the extraction of proteins. The lysis buffer consists of a dialyzable mild detergent, bicine, and 150 mM NaCl, resulting in minimal interference with protein interactions and biological activity (Figure 1). CelLytic MT is also used for extraction of cell-line proteins. A volume of 20 ml of CelLytic MT is sufficient for 1 gram of tissue.

It has been tested on the following tissues: rat brain, kidney, muscle, heart, liver, and spleen; mouse brain, kidney and muscle.

Product #  Product Name Package Size
C3228 CelLytic MT Cell Lysis Reagent 50 ml
250 ml

Figure 1. Gel Shift Assay of Oct-1.
Double Stranded 32P-labeled Oct-1 binding motif oligonucleotide was incubated with CelLytic MT extracts (4 µg total protein). Arrows indicate the Oct-1-DNA complex and free probe.

back to Mammalian Cell Lysis