Purification & Detection

HIS-Select Supporting Products Reagents

Products for UpStream Processes
Product Name Product No.
Cell Lysis Products  
CelLytic™ B Plus Kit, Lyse 50 grams of fresh or frozen cell paste CB0500
CelLytic™ B Plus Kit, Lyse up to 5 grams of fresh or frozen cell paste CB0050
CelLytic™ B Bacterial Cell Lysis/Extraction Reagent B3553
CelLytic™ B-II Bacterial Cell Lysis/Extraction Reagent B3678
CelLytic™-Y Yeast Cell Lysis/Extraction Reagent C4482
CelLytic™-M Mammalian Cell Lysis/Extraction Reagent C2978
CelLytic™ MT Mammalian Tissue Lysis/Extraction Reagent
C3228
CelLytic™ P Plant Cell Lysis/Extraction Reagent C2360
CelLytic™ PN Extraction Kit CelLyt-PN-1
CelLytic™ IB Inclusion Body Solubilization Reagent C5236
CelLytic™ NuCLEAR™ Extraction Kit N-XTRACT
Protease Inhibitor and Phosphatase Inhibitor Cocktails  
Poly (Histidine)-tagged Proteins Protease Inhibitor Cocktail P8849
Tissue Culture Media Protease Inhibitor Cocktail P1860
Mammalian Protease Inhibitor Cocktail, Animal Component Free (ACF) I3786
Mammalian Protease Inhibitor Cocktail P8340
SIGMAFAST™ Protease Inhibitor Cocktail Tablets S8830
MS-SAFE Protease and Phosphatase Inhibitor Cocktail MSSAFE
Phosphatase Inhibitor Cocktail I (DMSO solution)
P0044
 Phosphatase Inhibitor Cocktail II (aqueous solution) P5726
Competent Cells for Protein Expression  
BL21 Competent Cells Uni-Pack B8808
BL21(DE3) Competent Cells Uni-Pack B8683
BL21(DE3) PLYSS Competent Cells Uni-Pack B8933
BL21(DE3) PLYSE Competent Cells Uni-Pack B9058
Prepared Escherichia Coli Media  
LB Agar, Powder L2897
LB Agar EZMix™ Powder L7533
LB Agar, Tablet L7025
S-Gal™/LB Agar Blend C4478
LB Broth, Powder L3022
LB Broth EZMix™ Powder L7658
LB Broth, Tablet L7275
Luria Agar L3147
Luria Agar Base (Miller's Modification) L3272
Luria Broth L3522
Luria Broth (Miller's Modification) L3397
NZ Broth N3518
NZCYM Broth, Powder N3643
NZCYM Broth EZMix™ Powder N6905
M9 Minimal Salts, 5X M6030
Hanahan's Broth H8032
SOC Medium S1797
Terrific Broth, modified, Powder T0918
Terrific Broth, Modified EZMix™ Powder T9179
2X YT Microbial Medium, Powder Y2377
2X YT Microbial Medium EZMix™ Powder Y2627
Prepared Yeast Media  
YPD Agar Y1500
YPD Broth Y1375
Yeast Nitrogen Base With Amino Acids Y1250
Yeast Nitrogen Base without amino acids and ammonium sulfate Y1251
Yeast Nitrogen Base without amino acids Y0626
Yeast Synthetic Drop-out Medium Supplement Without Histidine Y1751
Yeast Synthetic Drop-out Medium Supplement Without Leucine Y1376
Yeast Synthetic Drop-out Medium Supplement Without Tryptophan Y1876
Yeast Synthetic Drop-out Medium Supplement Without Leucine and Tryptophan Y0750
Yeast Synthetic Drop-out Medium Supplement Without Uracil Y1501
Yeast Synthetic Drop-out Medium Supplement Without Histidine, Leucine, Tryptophan and Uracil Y2001
Products for DownStream Processes
Product Name Product No.
Bradford Reagent B6916
Bicinchoninic Acid Kit for Protein Determination BCA-1
Protein Standards, Micro Standard, Liquid P0914
EZBlue™ Gel Staining Reagent G1041
ProteoQwest™ Chemiluminescent Western Blotting Kit, CPS Substrate, (for Mouse Monoclonal IgG Antibodies) PQ0201
ProteoQwest™ Colorimetric Western Blotting Kit, TMB Substrate, (for Mouse Monoclonal IgG Antibodies) PQ0101
ProteoSilver™ Silver Stain Kit PROT-SIL1
ProteoSilver™ Plus Silver Stain Kit PROT-SIL2
ProteoMass™ Peptide and Protein MALDI-MS Calibration Kit MS-CAL1
ProteoMass™ Peptide MALDI-MS Calibration Kit MS-CAL2
ProteoMass™ Protein MALDI-MS Calibration Kit MS-CAL3
Trypsin from porcine pancreas, Proteomics Grade T6567
ProteoProfile™ Trypsin In-gel Digest Kit PP0100
Reagents and Products for use with HIS-Select™
Product Name Product No.
Polypropylene Chromatography Columns, General Purpose, Size: 10 mL, Overall : 5 in. C2103
Polypropylene Chromatography Columns, General Purpose, Size: 13 mL, Overall : 7 in. C2353
HIS-Select™ Elution Buffer H5413
HIS-Select™ Wash Buffer H5288
HIS-Select™ Wash & Elution Buffer Kit HS0100
Ethyl alcohol, HPLC grade, denatured 27,074-1
Glycerol anhydrous, for molecular biology, minimum 99% G5516
Guanidine hydrochloride, for molecular biology, minimum 99% G3272
L-Histidine monohydrochloride monohydrate, minimum 99% (TLC) H8000
Igepal CA-630, Viscous liquid I3021
Imidazole BioUltra, for molecular biology, ≥99.5% (GC) 56748
Imidazole for molecular biology, ≥99% (titration) I5513
Iminodiacetic acid agarose, Saline suspension I4758
2-Mercaptoethanolfor molecular biology, for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration) M3148
2-Mercaptoethanol BioUltra, for molecular biology, ≥99.0% (GC)
63689
Nickel(II) sulfate hexahydrate, Crystalline N4882
Sodium chloride, for molecular biology, minimum 98% (titration) S3014
Sodium phosphate monobasic dihydrate BioUltra, for molecular biology, ≥99.0% (T)
71505
Sodium phosphate monobasic BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.0% (titration)
S5011
Sodium phosphate monobasic BioReagent, for molecular biology, ≥anhydrous, 98%
S3139
Sodium phosphate monobasic Biotechnology Performance Certified, Cell Culture Tested
S6566
Sodium phosphate monobasic monohydrate BioXtra, for molecular biology, ≥99.5% (T)
71507
Trichloroacetic acid solution, 6.1 N T0699
Triton® X-100, SigmaUltra T9284
TWEEN® 20, for molecular biology, Viscous liquid P9416
Urea powder, BioReagent, for molecular biology, suitable for cell culture U5378

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