Purification & Detection

Magnetic Agarose Beads for Epitope Tag Protein Purifications


Sigma® now offers magnetic beads for immunoprecipitation, protein purifications, and the study of protein-protein interactions.

The magnetic properties allow for:

  • Very rapid separation
  • Significant accelerated manipulations, such as repetitive washings
  • Processing of multiple samples performed in plate formats

This leads to:

  • Faster experimentation
  • Better reproducibility
  • More accurate quantitation of the proteins of interest





Overview of Magnetic Beads

The magnetic properties allow for very rapid processing. These properties aid in manipulations, such as repetitive washings, and recovery of the protein bound beads. This leads to faster recovery, experimental reproducibility, and more accurate quantitation of the proteins of interest.

The matrix for these magnetic beads is 4% beaded agarose, with a diameter of 20-75mm, with an average diameter of 50mm. Superparamagnetic iron is impregnated within the bead. Beads are supplied in 50% glycerol with preservative and are stable for 2 years at recommended storage temperatures.



Anti-FLAG M2 Magnetic Beads

The FLAG Expression System is a proven method to express, purify and detect recombinant fusion proteins. The Anti-FLAG M2 Magnetic Bead (Product No. M8823) is composed of murine derived, anti-FLAG M2 monoclonal antibody attached to superparamagnetic iron impregated 4% agarose beads, with an average diameter of 50 µm. The M2 antibody is capable of binding to fusion proteins containing a FLAG peptide sequence at the N-terminus, Met-N-terminus, or C-terminus locations in mammalian, bacterial, and plant extracts. Binding attributes are similar to the standard Anti-FLAG M2 Agarose (Product No. A2220).

More information regarding FLAG and FLAG magnetic beads can be found at sigmaalrdrich.com/flagmag.



HIS-Select Nickel Magnetic Agarose Beads

HIS-Select Nickel Magnetic Agarose Beads (Product No. H9914) are designed to capture proteins with histidine tags while exhibiting low non-specific binding of other proteins. They can be used in a wide variety of cell lysates and other biochemical solutions in a manner similar to the standard HIS-Select Nickel Affinity Gel (Product No. P6611). Both contain a proprietary, quadridentate chelate, which prevents non-specific binding and nickel leaching. The capacity of the magnetic beads is typically greater than 10 mg/ml of packed gel, as determined with an approximately 30 kDa his-tagged protein.

More information regarding HIS-Select and HIS-Select magnetic beads can be found at sigmaaldrich.com/hismag.

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