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Roche Reagents for PCR - Selection Guide

 Enzymes for Routine and Hot Start

 

PCR Application Routine PCR Fast PCR Hot Start Multiplex/Sequencing
Your Enzyme of Choice: Taq DNA Polymerase AptaTaq Fast
DNA Polymerase
FastStart™ Taq
DNA Polymerase
FastStart High
Fidelity PCR System
Experience Consistency Speed Specificity Accuracy
Amplicon Size up to 3 kb up to 500 bp up to 3 kb up to 5 kb
Specificity
Sensitivity
Robustness
Accuracy vs. Taq 1x 1x 1x 4x
Carryover Prevention yes yes yes yes
Units/50 μl 1.25 2/20 μl 2 2.5
Molecular Cloning TA cloning TA cloning TA cloning TA cloning
Enzyme Taq DNA Polymerase,
   5 U/μl

Taq DNA Polymerase,
   1 U/μl
AptaTaq Fast DNA
  Polymerase, 1 U/μl
FastStart Taq DNA
  Polymerase, 5 U/μl
FastStart High Fidelity
  PCR System
Enzyme, GMP Grade • Taq DNA Polymerase,
   GMP Grade, 5 U/μl
     
dNTPack Taq DNA Polymerase,
  dNTPack, 5 U/μl

Taq DNA Polymerase,
  dNTPack, 1 U/μl
  FastStart Taq DNA
  Polymerase, dNTPack,
  5 U/μl
FastStart High Fidelity
  PCR System, dNTPack
Master Mix PCR Master AptaTaq Fast PCR Master FastStart PCR Master  

 

Enzymes for Specific Requirements

 

PCR Application Wide Variety/Easy Access High Fidelity Difficult Templates Long Range
Your Enzyme of Choice: Expand High Fidelity
PCR System
Pwo SuperYield DNA Polymerase GC-RICH PCR System Expand™ Long Range
Experience Robustness Fidelity Reliability Versatility
Amplicon Size up to 5 kb up to 3 kb up to 5 kb 5 to 25 kb**
Specificity
Sensitivity
Robustness
Accuracy vs. Taq 3x 18x 3x 3x
Carryover Prevention no* no no no
Units/50 μl 2.6 2.5 2 3.5
Molecular Cloning TA cloning Blunt end cloning TA cloning preferred over blunt end cloning TA cloning
Enzyme Expand High Fidelity
  PCR System

Expand High
  FidelityPLUS
  PCR System
Pwo SuperYield DNA
  Polymerase
GC-RICH PCR System  
dNTPack Expand High Fidelity
  PCR System, dNTPack

Expand High
  FidelityPLUS PCR
  System, dNTPack
Pwo SuperYield DNA
  Polymerase, dNTPack
GC-RICH PCR System,
  dNTPack
Expand Long Range,
  dNTPack
Master Mix High Fidelity PCR Master Pwo Master    

* Requiring carryover prevention and 6x higher fidelity? Choose Expand High FidelityPLUS PCR System.
** For amplicon sizes of 20-35 kb choose Expand 20 kbPLUS PCR System.

 

AptaTaq, FastStart, and Expand are all trademarks of Roche. For life science research only. Not for use in diagnostic procedures.