Attention:

Certain features of Sigma-Aldrich.com will be down for maintenance the evening of Friday August 18th starting at 8:00 pm CDT until Saturday August 19th at 12:01 pm CDT.   Please note that you still have telephone and email access to our local offices. We apologize for any inconvenience.

HyStem™ Cell Culture

HyStem-HP Cell Culture Scaffold Kits

Customizable, well characterized and consistent, HyStem-HP hydrogel cell culture scaffolds provide a complex, three dimensional environment in which cells are able to proliferate, much as they would in vivo.

Customizable
Each distinct cell type in a multicellular organism has its own unique microenvironment. HyStem-HP offers the flexibility to tailor the environments of cultured cells to mimic their in vivo counterparts and optimize proliferation and differentiation.

Synthetic
As a synthetic matrix, HyStem-HP offers greater control of the cellular environment than ambiguous extracted ECM alternatives. All components are well characterized and consistently formulated. HyStem-HP contains Hyaluronic acid (synthetic), Extralink (synthetic), Gelin-S (porcine), Heparin and Water.

Biologically accurate
Rich in hyaluronic acid and denatured collagen, the HyStem-HP hydrogel closely mimics the complex three dimensional cellular environments found in life, providing an optimal environment for culturing your cells.

Flexibility — HyStem-HP ensures complete control over:

  • Amount and type of GF incorporated
  • Cell encapsulation or plating on top of hydrogel
  • Cell-growth format (96- to 6-well plates and/or tissue-culture inserts)
  • Amount and type of ECM proteins incorporated
  • Hydrogel stiffness

Recommended for:
HyStem-HP is recommended for researchers planning to incorporate and gradually release growth factor into the stem cell environment.

Customize
With HyStem-HP, researchers can tailor the incorporation of ECM proteins and growth factors as well as the elasticity of the hydrogel and cell incorporation (encapsulations or top plating) to create the specific 3-D environment required by their cells.

Cell Types
The HyStem Hydrogel Kits are optimal for culturing stem cells whose natural environment is rich in hyaluronic acid (HA).

Cell Encapsulation
The HyStem platform accommodates cell encapsulations (true 3-D) as well as plating on top of the hydrogel for pseudo-3-D growth.

Cell Attachment
HyStem-C enables the attachment of a wide variety of cell types through Gelin-S (thiolated gelatin).

Growth Factor Incorporation
HyStem-HP enables ionic binding and slow release of growth factors through thiolated heparin; growth factors can be integrated via the matrix rather than cell culture medium. HyStem-HP also enables the attachment of a wide variety of cell types through Gelin-S (thiolated gelatin).

Components

  • HyStem HP (H2541)
  • Gelin-S (G3673)
  • Extralink 2 (E6659)
  • Water, degassed (W3894)

Formats
Trial Kit: (HYSHP010) for 2.5 ml of HyStem-HP Hydrogel
Standard Kit: (HYSHP020) for 7.5 ml of HyStem-HP Hydrogel

Explore
The HyStem-HP Trial Kit enables researchers to evaluate the HyStem-C technology in their own hands and confirm that HyStem-C is the right choice for their research.

Conditions
HyStem-HP is recommended for both in vivo and in vitro experimentation.

Gelation
Reconstituted HyStem-HP components remain liquid at 15 to 37 °C. The hydrogel is formed when Extralink is mixed with HyStem-HP and Gelin-S. Gelation occurs about twenty minutes after all three components are mixed. No steps depend on low temperature or low pH. Diluting the components with phosphate- buffered saline (PBS) or cell-culture medium can increase gelation time.

Similar Products
HyStem and HyStem-C
See the HyStem Selection Guide

back to top