HyStem™ Cell Culture

HyStem Selection Guide

  HyStem HyStem-C HyStem-HP
Recommended applications For researchers who require an animal component free system.

For researchers who will customize with their own attachment factors and/or ECM proteins/peptides

For researchers who require a minimal number of cell attachment sites
For researchers who require a large number of generalized cell attachment sites for their stem cell culture(s) For researchers planning to incorporate and gradually release growth factors into the stem cell environment
Customization • Amount and type of ECM proteins
• Amount and type of cell attachment factors
• Amount and type of growth factors
• Hydrogel rigidity
• Choice of cell encapsulation or top plating
• Amount and type of ECM proteins
• Amount and type of growth factors
• Hydrogel rigidity
• Choice of cell encapsulation or top plating
• Amount and type of ECM proteins
• Amount and type of growth factors
• Hydrogel rigidity
• Choice of cell encapsulation or top plating
Ordering Information HYS020 - HyStem Cell Culture Scaffold Kit (for 7.5 mL of hydrogel scaffold solution) 

HYS010 - HyStem Cell Culture Scaffold Trial Kit (for 2.5 mL of hydrogel scaffold solution)
HYSC020 - HyStem-C Cell Culture Scaffold Kit (for 7.5 mL of hydrogel scaffold solution) 

HYSC010 - HyStem-C Cell Culture Scaffold Trial Kit (for 2.5 mL of hydrogel scaffold solution)
HYSHP020 - HyStem-HP Cell Culture Scaffold Kit (for 7.5 mL of hydrogel scaffold solution) 

HYSHP010 - HyStem-HP Cell Culture Scaffold Trial Kit (for 2.5 mL of hydrogel scaffold solution)
Animal Component Free Yes No No
Cell Types tested • Human embryonic stem cells (H9s)
• Umbilical cord blood CD23+ stem cells
• Hepatic stem cells
• Hepatic progenitor cells
• Human embryonic stem cells
• Human mesenchymal stem cells
• Neural progenitor cells
• Hepatic progenitor cells
 
Appropriate conditions Recommended for in vivo and in vitro experimentation Recommended for in vitro experimentation only Recommended for in vivo and in vitro experimentation
Cell Encapsulation Accomodates cell encapsulation (true 3-D) as well as plating on top of the hydrogel for pseudo-3-D growth
Cell Attachment Offers a minimal number of cell attachment sites; if desired, researchers may incorporate attachment factors of their choice. Enables attachment of a wide variety of cell types through Gelin-S (thiolated gelatin) Enables attachment of a wide variety of cell types through Gelin-S (thiolated gelatin)
Growth Factor Incorporation Researchers will customize with their own growth factors; growth factors will likely require replenishment Researchers will customize with their own growth factors; growth factors will likely require replenishment Enables ionic binding and slow release of growth factors through thiolated heparin; frequent replenishment of growth factors is not required.
Gelation Time <20 minutes 20 minutes 23 minutes
Commercially available GMP counterpart Yes, available through Glycosan Biosciences; www.glycosan.com

Each Kit is sufficient to produce 7.5 ml of Hydrogel Scaffold
Each Trial Kit is sufficient to produce 2.5 ml of Hydrogel Scaffold

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