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3-D Stem Cell Culture

Other 3-D Matrices

Laminin


Laminin is an epithelial cell adhesion glycoprotein, which is composed of 3 chains designated α1, β1, and γ1. Laminin promotes adhesion, differentiation, migration, and growth of many cells in vitro. Laminin is produced in human fibroblasts and epithelial cells in a co-culture system, which produces an extracellular matrix composed of many proteins, including laminin. Laminin is then purified biochemically and provided as a sterile solution at a concentration of ~0.5 mg/mL in TBS, pH 7.4.

L4544-100UL – Laminin from human fibroblasts

L2020-1MG – Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane

Collagen


Collagen type IV creates an in vitro ECM (extracellular matrix) and serves as an excellent biological scaffold for 3-D cell cultures. Collagen IV is a heterotrimeric molecule containing two α1-like and one α2-like chain. It is considered essential for completion of embryogenesis and is necessary for proper tissue organization and structural integrity. It is used in vitro as a substrate to enhance adherence and proliferation of many cell types. Produced by human fibroblasts and epithelial cells. Collagen IV is then purified biochemically and provided as a sterile solution at a concentration of ~0.3 mg/mL in 25% acetic acid.

C6745-1ML – Collagen Type IV from human cell culture

C0543-1VL – Collagen from Engelbreth-Holm Swarm murine sarcoma basement membrane

Fibronectin


Fibronectin is a high molecular mass extracellular matrix glycoprotein involved in many cellular processes, including tissue repair, embryogenesis, blood clotting, and cell migration/ adhesion. It is also used in vitro as a substrate to enhance adherence and proliferation of many cell types. Fibronectin is produced by human fibroblasts, purified biochemically, and supplied as a sterile solution at a concentration of ~0.5 mg/mL in CAPS saline buffer.

F0556-100UL – Fibronectin solution from human fibroblasts


ECM Gel


ECM gel is composed primarily of laminin, collagen type IV, heparin sulfate proteoglycan and entacin. It is supplied as 8–12 mg/mL basement membrane matrix protein in Dulbecco’s Modified Eagle’s medium with 50 μg/mL gentamicin.

E1270-1ML – ECM gel from Engelbreth-Holm-Swarm murine sarcoma

E1270-5ML – ECM gel from Engelbreth-Holm-Swarm murine sarcoma

E1270-10ML – ECM gel from Engelbreth-Holm-Swarm murine sarcoma