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CHOZN® Media and Feed System

Screen Less, Produce More using CHOZN® Platform and EX-CELL® Advanced™ CHO Fed-batch System

SAFC's new EX-CELL Advanced media and feed system was designed and optimized for the CHOZN GS cell line using multiple molecules, including mAbs and fusion proteins, resulting in 3-5 fold increases in therapeutic productivity while maintaining critical protein attributes. The EX-CELL Advanced system has been shown to support in excess of 5g/L in CHOZN platform cell line which is achieved by a simple fed-batch, single feed scheme. In addition, the feed is stable at room temperature for >30 days, which allows for even greater time savings by allowing safety testing in parallel and prior to addition to the culture. Decrease your time to market and costs by leveraging the CHOZN manufacturing system!

Platform Media and Feeds

Optimized media and feeds that can be purchased separately as part of the CHOZN platform are listed below.  Please see CHOZN Platform technical bulletin for details on media use.

Product No. Description Product Information Sheet
14366C EX-CELL® Advanced™ CHO Fed-batch Medium – Liquid PIS (PDF)
24366C EX-CELL® Advanced™ CHO Fed-batch Medium – Powder PIS (PDF)
24367C EX-CELL® Advanced™ CHO Feed 1 (with glucose) PIS (PDF)
24368C EX-CELL® Advanced™ CHO Feed 1 (without glucose) PIS (PDF)
14365C EX-CELL® CD CHO Fusion Medium
PIS (PDF)
C6366 EX-CELL® CHO Cloning Medium Poster (PDF)
Growth/Scale Up Stage Media Required
CHOZN GS thawing and subculturing 14365C CD Fusion
Transfection

14365C CD Fusion

Selection using “minipools” 14365C CD Fusion
C6366 Cloning media
Recovery, screening in static 96-well plates, scale up to TPP spintubes/shake flasks 14365C CD Fusion
Fed- batch assay on minipools (TPP tubes or shake flasks) EX-CELL® Advanced™ CHO Fed Batch Medium (14366C or 24366C)
EX-CELL® Advanced™ CHO Feed 1 (w/ or w/out glucose) 24367C, 24368C)
Bank top producing minipools 14365CCD Fusion +7% DMSO
Single cell clone top producing minipools 14365C CD Fusion
Static 96-well screen on single cell clones 14365C CD Fusion
Top clones scaled up to TPP/Shake flasks 14365C CD Fusion
Top clones screened in Fed-Batch Assay (TPP spintubes/shake flasks) EX-CELL® Advanced™ CHO Fed Batch Medium (14366C or 24366C)
EX-CELL® Advanced™ CHO Feed 1 (w/ or w/out glucose) 24367C, 24368C)
Top clones banked 14365C CD Fusion +7% DMSO
Seed train 14365C CD Fusion
Bioreactor studies EX-CELL® Advanced™ CHO Fed Batch Medium (14366C or 24366C)
EX-CELL® Advanced™ CHO Feed 1 (w/ or w/out glucose) 24367C, 24368C)
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Learn more about the CHOZN Expression Platform
Download Technical Bulletin
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