GC Analysis of 1,3-Dimethylamylamine (DMAA) Enantiomers (N-Pentafluoropropionyl Derivatives) on Astec® CHIRALDEX® G-DM, 30 ºC Oven Temp.

GC Analysis of 1,3-Dimethylamylamine (DMAA) Enantiomers (N-Pentafluoropropionyl Derivatives) on Astec® CHIRALDEX® G-DM, 30 ºC Oven Temp.

Conditions

column Astec CHIRALDEX G-DM, 30 m x 0.25 mm I.D., 0.12 µm particles (77033AST)
oven 30 C (isothermal)
carrier gas helium, 1 ml/min, constant
injection 1.0 muL100:1 split
inj. temp. 250 C
detector FID, 250 C

Description

Analysis Note 1,3-Dimethylamylamine (DMAA) is a stimulant found in various health supplements. Although often labelled as "natural," derived from geranium plants, it is suspected of actually having synthetic origins. If synthetic, then the supplement would fall under FDA regulations. In this study, chiral GC was used to measure the enantiomeric and diastereomeric ratios of synthetic DMAA. Shown here are the two sets of enantiomers of DMAA separated by GC on Astec CHIRALDEX G-DM.
Categories Analytical Chromatography, GC Applications, Enantiomers, Pharmaceuticals
Featured Industry Clinical
Food and Beverages
Forensics and Toxicology
Pharmaceutical (small molecule)
Legal Information CHIRALDEX is a registered trademark of Sigma-Aldrich Co. LLC, Astec is a registered trademark of Sigma-Aldrich Co. LLC
Other Notes 10 mg of DMAA standard and 10 mg of internal standard (2-aminopentane) were dissolved in 0.5 mL of dichloromethane. 0.5 mL PFPA was added to the vial and the vial was sealed with a silicone rubber insert. The solution was heated for 30 min at 50 °C. Then the solvent and residual PFPA were removed at room temperature under reduced pressure. The derivatized DMAA and internal standard were transferred to a 10 mL volumetric flask and diluted to 10 mL with heptane.
suitability application for GC

Analyte

  • 1,3-Dimethylamylamine (DMAA)

Materials

     
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