GC Analysis of a 20-Component Organochlorine Pesticide Mix in Water on a 5% Phenyl Column after SPE using Supelclean™ ENVI™-8

Conditions

sample preparation SPE (Solid Phase Extraction)
sample/matrix waste water spiked with pesticides
SPE tube/cartridge Supelclean ENVI-8, glass hardware, 500 mg/6 mL (57107 (500 mg/6 mL); 57108 (1 g/6 mL))
condition 3 mL methanol; h 2 mL 5% methanol in water
sample addition load sample at 5 mL/min
drying 2-3 min with nitrogen stream
elution 2 x 4 mL hexane:acetone (90:10), drop wise. allow to soak into bed
column 5% Phenyl Polysiloxane, 15 m x 0.53 mm I.D., 0.50 µm (Supelco equivalent, Equity-5, 28252-U)
oven 150 °C (0.5 min) to 275 °C (5 min)
carrier gas helium
injection 1 µLsplit/splitless (45 sec delay)
inj. temp. 200 °C
detector ECD, 300 °C

Description

Application Sample Pre-treatment:
Add 5 mL methanol to 100 mL aqueous hazardous waste. Adjust to pH 5-7 if necessary.
Categories Analytical Chromatography, SPE Applications, GC Applications, Pesticides
Featured Industry Environmental
Food and Beverages
Legal Information ENVI is a trademark of Sigma-Aldrich Co. LLC, Supelclean is a trademark of Sigma-Aldrich Co. LLC
Other Notes Complementary products:
Syringes
Vials
Gas Purification and Gas Management
Inlet Liners, Glass Wool, and Specialized Hand Tools
Septa and Specialized Hand Tools
Column Ferrules, Nuts, and Specialized Hand Tools
suitability application for GC, application for SPE

Analyte

  • 1,1,1-Trichloro-2,2-bis(4-chlorophenyl)ethane (4,4′-DDT)
  • 1,1-Dichloro-2,2-bis(4-chlorophenyl)ethane (4,4′-DDD)
  • 4,4′-DDE (1,1-Dichloro-2,2-bis(p-chlorophenyl)ethylene)
  • Aldrin
  • Decachlorobiphenyl
  • Dieldrin
  • Endosulfan I
  • Endosulfan II
  • Endosulfan sulfate
  • Endrin
  • Endrin aldehyde
  • Endrin ketone
  • Heptachlor
  • Heptachlor epoxide
  • Methoxychlor
  • α-BHC
  • α-Chlordane
  • β-BHC
  • γ-BHC
  • γ-Chlordane
  • δ-BHC

Materials

     
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