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GC Analysis of Aroma Fusel Alcohols in Grappa Sample 1 on Watercol™ 1910 after Headspace SPME using 75 μm Carboxen®/PDMS Fiber, MSD

GC Analysis of Aroma Fusel Alcohols in Grappa Sample 1 on Watercol™ 1910 after Headspace SPME using 75 μm Carboxen®/PDMS Fiber, MSD

Conditions

sample preparation Solid Phase Microextraction
sample/matrix 0.5 mL grappa sample 1 in a 2 mL vial
desorption process 300 C for 1.1 min
extraction headspace, 60 C for 45 min
SPME fiber 75 µm Carboxen(R)/polydimethylsiloxane on a fused silica core (57318)
column Watercol 1910, 30 m x 0.25 mm I.D., 0.20 µm ; two identical columns installed in the same inlet, each going to a separate detector (29711-U)
oven 40 C (2 min), 4 C/min to 125 C (1 min)
carrier gas helium, 1.1 ml/min
injection splitless
liner 0.75 mm I.D., direct (SPME) type, straight design (2633901)
inj. temp. 300 C
detector MSD, m/z = 18-500
MSD interface 260 C

Description

Analysis Note Fusel alcohols are by-products of the fermentation process. Four of these (n-propanol, isobutanol, active amyl alcohol, and isoamyl alcohol) are important to alcoholic beverage manufacturers due to their aroma characteristics.

The "Watercol" series of ionic liquid capillary GC columns are characterized by their ability to produce a sharp peak shape for water, sharp enough so that water can be integrated and quantified. Sharp peak shapes are also produced for many other small polar analytes, such as the four aroma fusel alcohols. In this application, a brandy sample was analyzed for the presence of aroma fusel alcohols. As shown, great peak shapes were obtained, resulting in good resolution.

In addition to sharp fusel alcohol peaks, the column′s selectivity results in elution of both ethanol and water free from all analytes.
Categories Analytical Chromatography, SPME Applications, Food and Beverage
Featured Industry Food and Beverages
Legal Information Watercol is a trademark of Sigma-Aldrich Co. LLC, Carboxen is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for SPME, application for GC

Materials

     
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