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GC Analysis of Bacterial Acid Methyl Esters (BAMEs) on SUPELCOWAX® 10 (15 m x 0.10 mm I.D., 0.10 μm), Fast GC Analysis

GC Analysis of Bacterial Acid Methyl Esters (BAMEs) on SUPELCOWAX® 10 (15 m x 0.10 mm I.D., 0.10 μm), Fast GC Analysis

Conditions

column SUPELCOWAX 10, 15 m x 0.10 mm I.D., 0.10 µm (24343)
oven 80C (0.3 min), 30 C/min to 180 C (0.5 min), 10 C/min to 200 C (1 min)
carrier gas hydrogen, 45 cm/sec, constant
sample bacterial methyl ester standards in methyl caproate, 10 mg/mL (47080-U)
injection 0.1 muL200:1 split
liner 2 mm I.D., straight
inj. temp. 250 C
detector FID, 250 C

Description

Analysis Note Food-borne microbial pathogens can cause illness in humans. Because each bacterium has a unique cellular fatty acid profile, GC analysis can be used to help with identification. Analysis is performed following derivatization of cellular fatty acids to methyl esters. These are called bacterial acid methyl esters (BAMEs) instead of fatty acid methyl esters (FAMEs) to signify the bacterial source. The chromatogram shown is the analysis of a standard mix containing 26 BAME compounds. The polar nature of the column, along with the use of Fast GC column dimensions and instrument conditions, allows good peak shape and resolution in just 7 minutes.
Categories Analytical Chromatography, GC Applications, Bacterial Acid Methyl Esters (BAMEs)
Featured Industry Clinical
Legal Information SUPELCOWAX is a registered trademark of Sigma-Aldrich Co. LLC
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Materials

     
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