GC Analysis of FAMEs in Cookies on SP™-2560 after SPE Fractionation using Discovery® Ag-Ion

GC Analysis of FAMEs in Cookies on SP™-2560 after SPE Fractionation using Discovery® Ag-Ion

Conditions

sample preparation SPE (Solid Phase Extraction)
sample/matrix 1 g of commercially purchased cookies was ground and subjected to acid digestion and alkali hydrolysis followed by methylation as described in AOCS Official Method Ce 1k-09
SPE tube/cartridge Discovery Ag-Ion SPE tubes, 750 mg/6 mL (54225-U)
condition 4 mL of acetone; allow solvent to gravity drip completely through tube; discard eluant; 4 mL of hexane; allow solvent to gravity drip completely through tube; discard eluant
sample addition 1 mL of extract; discard any eluant that drips through tube
elution (Fraction 1) 6 mL of hexane:acetone (96:4); collect eluant in a fresh container with slight vacuum; ((Fraction 2) 4 mL of hexane: acetone (90:10); collect eluant in a fresh container with slight vacuum;(Fraction 3) 4 mL of 100% acetone; collect eluant in a fresh container with slight vacuum)
eluate post-treatment evaporate each fraction at room temperature using nitrogen; reconstitute each fraction to 1 mL of hexane
column SP-2560, 100 m x 0.25 mm I.D., 0.20 µm (24056)
oven 180 C
carrier gas hydrogen, 1 ml/min
injection 1 µL, 10:1 split
liner 4 mm I.D., split type, single taper wool packed FocusLiner(TM) design
inj. temp. 250 C
detector FID, 250 C

Description

Analysis Note A sample of commercially purchased cookies were prepared using an acid digestions /alkali hydrolysis followed by methylation as described in AOCS Official Method Ce 1k-09 (1). The resulting extracts were then fractionated using Ag-ion SPE and analyzed by GC/FID on the 100 m SP-2560 capillary column.
Categories Analytical Chromatography, SPE Applications, GC Applications, FAMEs (Fatty Acid Methyl Esters), Fatty Acid Methyl Esters (FAMEs)
Featured Industry Food and Beverages
Legal Information FocusLiner is a trademark of SGE Analytical Science Pty Ltd., Discovery is a registered trademark of Sigma-Aldrich Co. LLC, SP is a trademark of Sigma-Aldrich Co. LLC
Other Notes Complementary products:
Syringes
Vials
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suitability application for GC, application for SPE

Materials

     
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