Attention:

Certain features of Sigma-Aldrich.com will be down for maintenance the evening of Friday August 18th starting at 8:00 pm CDT until Saturday August 19th at 12:01 pm CDT.   Please note that you still have telephone and email access to our local offices. We apologize for any inconvenience.

GC Analysis of FAMEs in Walnuts on SLB®-IL111

GC Analysis of FAMEs in Walnuts on SLB®-IL111

Conditions

column SLB-IL111, 100 m x 0.25 mm I.D., 0.20 µm (29647-U)
oven 168 C
carrier gas FID, 250 C
sample ground walnuts prepared for analysis per AOCS Official Method Ce 1k-09
injection 1 µL, split 100:1
liner 4 mm ID FocusLiner w/taper
inj. temp. 250 C
detector FID, 250 C

Description

Analysis Note This application illustrates the extraction and GC analysis of walnuts for fatty acids. Walnut is considered a significant plant source of alpha-linolenic acid (ALA). Both ALA and linoleic acid (LA) are the predominant essential fatty acids in humans (1). The samples were prepared using acid digestion /alkali hydrolysis followed by methylation as described in AOCS Official Method Ce 1k-09 (2). BHT was added as an antioxidant prior to extraction. All samples were concentrated to 1 mL prior to GC analysis. The FAME composition of vegetable oils is normally determined using AOCS Method Ce 1h-05, which designates the use of the a cyanopropylsilicone column (3). The SLB-IL111 is a highly polar column which provides alternate selecitivty and resolution of geometric positional isomers of fatty acid methyl esters. It can be used to complement the method designated column.
Categories Analytical Chromatography, GC Applications, Fatty Acid Methyl Esters (FAMEs)
Featured Industry Agriculture
Food and Beverages
Legal Information SLB is a registered trademark of Sigma-Aldrich Co. LLC
Other Notes Complementary products:
Syringes
Vials
Gas Purification and Gas Management
Inlet Liners, Glass Wool, and Specialized Hand Tools
Septa and Specialized Hand Tools
Column Ferrules, Nuts, and Specialized Hand Tools
suitability application for GC

Materials

     
Related Links