GC Analysis of PCBs in Transformer Oil on SPB®-5 after SPE using Supelclean™ LC-Florisil® and LC-Si

GC Analysis of PCBs in Transformer Oil on SPB®-5 after SPE using Supelclean™ LC-Florisil® and LC-Si

Conditions

sample preparation SPE (Solid Phase Extraction)
sample/matrix used transormer oil containing 50 ppb Aroclor 1254.
SPE tube/cartridge Supelclean LC-Florisil, 1.0 g/6 mL (57057)
SPE tube/cartridge Supelclean LC-Si, 1.0 g/6 mL (57051)
condition 0.5 mL isooctane (Florisil tube)
sample addition 0.2 g transformer oil or 0.1 g waste oil
washing 5 x 2 mL isooctane
column SPB-5, 30 m x 0.32 mm I.D., 0.25 µm film (24048)
oven 40 °C (4 min) to 300 °C at 10 °C/min, hold 5 min
carrier gas nitrogen
injection 1 µL, splitless (30 sec delay), then split (50:1)
detector ECD

Description

Application Connect Supelclean LC-Si SPE tube (1 g/6 mL) in series to male luer outlet of Supelclean LC-Florisil SPE tube (1 g/6 mL) using an SPE Tube Adapter (57020-U). Apply up to 0.2 g transformer oil or 0.1 g waste oil to upper frit of Florisil SPE tube. Pass 5 x 2 mL isooctane through both Florisil and LC-Si SPE tubes. Oil interferences will be retained on SPE tubes whereas PCBs will elute with isooctane fractions. Collect and combine isooctane fractions using 10 mL volumetric flask. Analyze isooctane extract using GC-ECD.
Categories Analytical Chromatography, SPE Applications, GC Applications, Dioxins, PCBs, PBDEs, Dioxins/PCBs/PBDEs
Featured Industry Environmental
Legal Information SPB is a registered trademark of Sigma-Aldrich Co. LLC, Supelclean is a trademark of Sigma-Aldrich Co. LLC, Florisil is a registered trademark of U.S. Silica Company
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suitability application for GC, application for SPE

Materials

     
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