GC Analysis of PCBs in Whole Cow Milk (Spiked) on SLB®-5ms (20 m x 0.18 mm I.D., 0.18 μm) after QuEChERS Cleanup using Supel™ QuE Z-Sep, Fast GC Analysis

GC Analysis of PCBs in Whole Cow Milk (Spiked) on SLB®-5ms (20 m x 0.18 mm I.D., 0.18 μm) after QuEChERS Cleanup using Supel™ QuE Z-Sep, Fast GC Analysis

Conditions

sample preparation SPE (Solid Phase Extraction)
sample/matrix 10 g whole cow's milk
extraction tube Supel™ QuE Non-buffered tube (4 g magnesium sulfate, 1 g sodium chloride) (55294-U)
clean-up tube Supel™ QuE Z-Sep 150 mg, magnesium sulfate (150 mg, part no. 63135) (55299-U)
column SLB-5ms, 20 m x 0.18 mm I.D., 0.18 µm (28564-U)
oven 75 °C (1 min), 12 °C/min to 340 °C (10 min)
carrier gas hydrogen, 1.2 mL/min, constant
injection 1 µLsplitless (0.75 min)
liner 4 mm I.D. FocusLiner™ with taper
inj. temp. 250 °C
detector mu-ECD, 340 °C

Description

Analysis Note This application demonstrates the use of dispersive SPE or "QuEChERS" for the extraction and cleanup of whole cow′s milk for GC-ECD analysis of polychlorinated biphenyl (PCBs). Z-Sep sorbent was used in the cleanup step and found to reduce background and yield recoveries >70% for all PCB congeners evaluated except no. 209, which was recovered at 68%.
Extraction solvent: acetone:hexane (1:1), 20 mL
Clean-up volume: 1 mL
Categories Analytical Chromatography, Food & Beverage Analysis, SPE Applications, GC Applications, Dioxins, PCBs, PBDEs, Beverage Testing, Dioxins/PCBs/PBDEs, Milk
Featured Industry Food and Beverages
General description Reference: Stenerson, K.; Ye, M.; Halpenny, M., New Analytical Tools for the Determination of Persistent Organic Pollutants (POPs) in Fatty Food and Beverage Matrices Using QuEChERS Extraction/Cleanup and Gas Chromatography (GC) Analysis. Pittcon 2013. Sigma-Aldrich/Supelco publication T413034
Legal Information Supel is a trademark of Sigma-Aldrich Co. LLC, FocusLiner is a trademark of SGE Analytical Science Pty Ltd., SLB is a registered trademark of Sigma-Aldrich Co. LLC
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suitability application for GC, application for SPE

Materials

     
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