GC Analysis of Pesticides in Beef Kidney on SLB®-5ms (20 m x 0.18 mm I.D., 0.36 μm) after QuEChERS Cleanup using Supel™ Que Z-Sep+, MS in SIM Mode, Fast GC Analysis

GC Analysis of Pesticides in Beef Kidney on SLB®-5ms (20 m x 0.18 mm I.D., 0.36 μm) after QuEChERS Cleanup using Supel™ Que Z-Sep+, MS in SIM Mode, Fast GC Analysis

Conditions

sample preparation Dispersive (QuEChERS)
sample/matrix 10 gm homogenized beef kidney spiked at 50 ng/g with pesticides
extraction tube Supel Que Acetate (AC) Tube (55234-U)
extraction process add 10 mL of acetonitrile; shake for 1 minute; add contents of Supel Que acetate extraction tube (55234-U); shake immediately for 1 minute; centrifuge at 3200 rpm for 5 minutes; (Sample Pretreatment: Homogenize 10 gm beef kidney and spike with pesticides at 50 ng/g.)
clean-up process transfer 3 mL of the acetonitrile layer into a Supel QuE Z-Sep+ cleanup tube (55296-U); shake for 1 minute; centrifuge at 3400 rpm for 3 minutes; draw off supernatant for LVI-GCMS analysis
clean-up tube Supel QuE Z-Sep+ tube (55296-U)
column F.S. CAP. SLB-5MS 20 m x 0.18 mm I.D., 0.36 µm (28576-U)
oven 70 C ( 0.5 min), 25 C/min to 125 C, 10 C/min to 200 C, 5 C/min to 300 C/min (1 min)
carrier gas helium, 1 ml/min constant flow
sample Beef Kidney extract
injection 10 µl LVI, PTV solvent vent, rapid injection speed
liner 4 mm ID FocusLiner with taper
inj. temp. programmed, 60 C (0.28 min), 600 C/min to 325 C (5 min)
detector MSD
MSD interface 325 C

Description

Analysis Note Beef kidney was analyzed for pesticides by GC/MS-SIM after extraction and cleanup. Dispersive SPE was used for both extraction and cleanup. Since beef kideny contains approximately 5% fat, Z-Sep + was used for cleanup.
Categories Analytical Chromatography, SPE Applications, GC Applications, Pesticides
Featured Industry Food and Beverages
Legal Information Supel is a trademark of Sigma-Aldrich Co. LLC, SLB is a registered trademark of Sigma-Aldrich Co. LLC
Other Notes Complementary products:
Syringes
Vials
Gas Purification and Gas Management
Inlet Liners, Glass Wool, and Specialized Hand Tools
Septa and Specialized Hand Tools
Column Ferrules, Nuts, and Specialized Hand Tools
suitability application for GC, application for SPE

Analyte

  • 4,4′-DDD
  • 4,4′-DDE (1,1-Dichloro-2,2-bis(p-chlorophenyl)ethylene)
  • 4,4′-DDT
  • Bifenthrin
  • Cyfluthrin isomers
  • Cyhalothrin
  • Diphenylamine
  • Endosulfan I
  • Endosulfan II
  • Endosulfan sulfate
  • Hexachlorobenzene
  • Permethrin isomers
  • Piperonyl butoxide
  • γ-BHC

Materials

     
Related Links