GC Analysis of Pesticides in Fruits and Vegetables on a 14%Cyanopropylphenyl Column after SPE using Supelclean ENVI-Carb™

GC Analysis of Pesticides in Fruits and Vegetables on a 14%Cyanopropylphenyl Column after SPE using Supelclean ENVI-Carb™

Conditions

sample preparation SPE (Solid Phase Extraction)
sample/matrix extract from 50 g homogenized fruit or vegetable sample
SPE tube/cartridge ENVI-Carb, 500 mg/6 mL (57094)
sample addition 0.5 mL concentrated sample extract
elution 20 mL acetonitrile:toluene (3:1)
eluate post-treatment Concentrate to 2 mL by rotary evaporation. Add 2 x 10 mL acetone concentrating to 2 mL after each acetone addition. (Add 50 µl I.S. (500 ng/µl cis-chlordane in acetone) then dilute to 2.5 mL with acetone.)
column 14% cyanopropylphenyl polysiloxane, 30m x 0.25 mm I.D., 0.15 µm (Supelco equivalent, Equity-1701, 28372-U)
oven 70 C (hold 2 min) to 130 C at 25 C; 130 C to 220 C at 2 C/min; 220 C to 280 C at 10 C/min and hold 4.6 min
carrier gas helium
injection 2 µL, splitless
detector MSD, 285 C

Description

Application Sample Pre-treatment:
Homogenize 50 g chopped fruit/vegetable sample in 100 mL acetonitrile. Add 10 g sodium chloride and homogenize 5 min. Transfer 13 mL of acetonitrile layer to clean centrifuge tube. Centrifuge at high speed for 5 min. Evaporate 10 mL aliquot of supernatant to 0.5 mL under Nitrogen at 35°C.
Categories Analytical Chromatography, Food & Beverage Analysis, SPE Applications, GC Applications, Pesticide and Metabolite Residues, Pesticides, Applications (Chromatograms)
Featured Industry Agriculture
Food and Beverages
Legal Information ENVI-Carb is a trademark of Sigma-Aldrich Co. LLC
Other Notes Complementary products:
Syringes
Vials
Gas Purification and Gas Management
Inlet Liners, Glass Wool, and Specialized Hand Tools
Septa and Specialized Hand Tools
Column Ferrules, Nuts, and Specialized Hand Tools
suitability application for GC, application for SPE

Materials

     
Related Links