GC Analysis of Polynuclear Aromatic Hydrocarbons (PAHs) in Grilled Hamburger on SPB®-608 (20 m x 0.18 mm I.D., 0.18 μm) after QuEChERS Cleanup using Supel™ QuE Z-Sep, Fast GC Analysis

GC Analysis of Polynuclear Aromatic Hydrocarbons (PAHs) in Grilled Hamburger on SPB®-608 (20 m x 0.18 mm I.D., 0.18 μm) after QuEChERS Cleanup using Supel™ QuE Z-Sep, Fast GC Analysis

Conditions

sample preparation Dispersive (QuEChERS)
sample/matrix grilled hamburger spiked with PAHs at 100 ng/g
extraction tube Sodium chloride/magnesium sulfate (55294-U)
extraction process add 25 mL acetonitrile, contents of unbuffered salt tube (55294-U); shake for 5 minutes; centrifuge for 5 minutes at 3200 rpm
clean-up process transfer 3 mL of acetonitrile layer into a Supel QuE Z-Sep cleanup tube (55403-U); shake 1 minute; centrifuge for 3 minutes at 3400 rpm; remove 1 mL supernatant for GC-MS analysis
clean-up tube Supel QuE Z-Sep tube (55403-U)
column SPB-608, 20 m x 0.18 mm I.D. x 0.18 µm
oven 60 °C (1 min), 25 °C/min to 275 °C, 10 °C/min to 300 °C (13 min)
carrier gas helium, 1.5 mL/min constant flow
sample grilled hamburger extract
injection 1 µLsplitless (1 min.)
liner 4 mm I.D. FocusLiner™ with taper
inj. temp. 265 °C
detector MS-SIM
MSD interface 300 °C

Description

Analysis Note Sample pretreatment: Place 5 g ground hamburger sample into 50 mL centrifuge tube (55248-U). Spike with 100 ng/g target PAHs and internal standards. Let sit 10 min. Add 5 mL water and homogenize., This application demonstrates the use of dispersive SPE or QuEChERS for the extraction and cleanup of grilled hamburger for GC-MS analysis of polynuclear aromatic hydrocarbons (PAHs). Z-Sep sorbent was used in the cleanup step and found to reduce background and yield recoveries >80% for a majority of the PAHs tested.
Categories Analytical Chromatography, SPE Applications, GC Applications, Hydrocarbons, PAHs
Featured Industry Food and Beverages
Legal Information SPB is a registered trademark of Sigma-Aldrich Co. LLC, Supel is a trademark of Sigma-Aldrich Co. LLC, FocusLiner is a trademark of SGE Analytical Science Pty Ltd.
Other Notes Complementary products:
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suitability application for GC, application for SPE

Materials

     
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