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GC Analysis of Pyrazines in Peanut Butter on SUPELCOWAX® 10 after SPME using 50/30 μm DVB/Carboxen/PDMS Fiber

GC Analysis of Pyrazines in Peanut Butter on SUPELCOWAX® 10 after SPME using 50/30 μm DVB/Carboxen/PDMS Fiber

Conditions

sample/matrix 5 g peanut butter in 40 mL vial
desorption process 270 C for 5 min
extraction headspace, 30 min at 65 C in heating block
SPME fiber divinylbenzene-Carboxen-polydimethylbenzene StableFlex (57328-U)
column SUPELCOWAX 10, 30 m x 0.25 mm, 0.25 µm (24079)
oven 40 C (5 min) to 230 C at 4 C/min
carrier gas Helium, 30 cm/sec
sample peanut butter
injection splitless/split, closed 0.5 min, 270 C, with 0.75 mm liner
liner 0.75 mm
inj. temp. 270 C
detector ion trap mass spectrometer, m/z =30-350 at 0.6 sec/scan. Selected ions used for quantitation.

Description

Analysis Note In this application, SPME was used for the analysis of volatiles in multifloral honey using a headspace method. Headspace SPME (HS-SPME) using a Carboxen/PDMS/DVB fiber coupled with gas chromatography-mass spectrometry (GC/MS) was used for the characterization of the volatile fraction of honey samples. Using SPME it was possible to obtain and distinguish the chemical fingerprints of different types of honey. The relatively polar SUPELCOWAX 10 GC column provides excellent separation of these polar compounds.
Categories Analytical Chromatography, SPME Applications, GC Applications, Foods, Flavors & Fragrances, Volatiles
Featured Industry Agriculture
Food and Beverages
Legal Information SUPELCOWAX is a registered trademark of Sigma-Aldrich Co. LLC
Other Notes Complementary products:
Syringes
Vials
Gas Purification and Gas Management
Inlet Liners, Glass Wool, and Specialized Hand Tools
Septa and Specialized Hand Tools
Column Ferrules, Nuts, and Specialized Hand Tools
suitability application for SPME, application for GC

Analyte

  • 1-Methylpyrrole
  • 2,3-Diethylpyrazine
  • 2,3-Dimethylpyrazine
  • 2,5-Dimethylpyrazine
  • 2,6-Diethyl pyrazine
  • 2,6-Dimethylpyrazine
  • 2-Allyl-5-methylpyrazine
  • 2-Ethenyl-5-methylpyrazine
  • 2-Ethenyl-6-methylpyrazine
  • 2-Ethyl pyrazine
  • 2-Ethyl-3,5-dimethyl pyrazine
  • 2-Ethyl-3-methyl pyrazine
  • 2-Ethyl-5-methyl pyrazine
  • 2-Ethyl-6-methyl pyrazine
  • 2-Methyl-5-isopropylpyrazine
  • 2-Methyl-5-propylpyrazine
  • 2-Methyl-6-cis propenylpyrazine
  • 2-Methylpyrazine
  • 3,5-Diethyl-2-methyl pyrazine
  • 3-Ethyl-2,5-dimethylpyrazine
  • 3-Methylbutanal
  • 4-Methylpentene-2-one
  • 5-Methyl-2-propylpyrazine
  • Carbon disulfide
  • Dimethyl disulfide
  • Heptanal
  • Hexanal
  • Pentanal
  • Trimethyl pyrazine

Materials

     
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